Cloned (Comment) | Organism |
---|---|
expression of His8-tagged isozymes in Escherichia coli strain BL21 | Encephalitozoon cuniculi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | at early parasite life cycle | Encephalitozoon cuniculi | 9986 | - |
cell wall | localized in the microsporidian cell wall | Encephalitozoon cuniculi | 5618 | - |
additional information | at the sporoblast stage the enzyme accumulates in paramural bodies which are convolutions of the plasmembrane opened to the wall | Encephalitozoon cuniculi | - |
- |
plasma membrane | outer face, at early parasite life cycle | Encephalitozoon cuniculi | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, isozyme 1, SDS-PAGE, x * 55000, isozyme 2, SDS-PAGE | Encephalitozoon cuniculi |
55000 | - |
x * 33000, isozyme 1, SDS-PAGE, x * 55000, isozyme 2, SDS-PAGE | Encephalitozoon cuniculi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Encephalitozoon cuniculi | - |
two isozymes | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme contains an N-terminal signal sequence which is cleaved at Gly15/Ser16 | Encephalitozoon cuniculi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
microspore | - |
Encephalitozoon cuniculi | - |
additional information | spores are produced in MDCK cells | Encephalitozoon cuniculi | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, isozyme 1, SDS-PAGE, x * 55000, isozyme 2, SDS-PAGE | Encephalitozoon cuniculi |
More | peptide mass fingerprinting of both isozymes, MALDI-TOF mass spectrometry, the enzyme has a large C-terminal extracellular domain and a transmembrane domain | Encephalitozoon cuniculi |
Synonyms | Comment | Organism |
---|---|---|
CDA | - |
Encephalitozoon cuniculi |
More | the enzyme belongs to the polysaccharide dacetylase family | Encephalitozoon cuniculi |