BRENDA - Enzyme Database
show all sequences of 3.5.1.40

Preparation of bleomycinic acid: Hydrolysis of bleomycin B2 by a Fusarium acylagmatine amidohydrolase

Umezawa, H.; Takahashi, Y.; Fujii, A.; Saino, T.; Shirai, T.; Takita, T.; J. Antibiot. 26, 117-119 (1973)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.22
-
benzoyl agmatine
-
Fusarium anguioides
0.8
-
bleomycin B2
-
Fusarium anguioides
1.33
-
acetyl agmatine
-
Fusarium anguioides
2
-
propionyl agmatine
-
Fusarium anguioides
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Fusarium anguioides
5829
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Fusarium anguioides
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Fusarium anguioides
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Fusarium anguioides
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.14
-
-
Fusarium anguioides
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl agmatine + H2O
-
172038
Fusarium anguioides
acetic acid + agmatine
-
172038
Fusarium anguioides
?
benzoyl agmatine + H2O
-
172038
Fusarium anguioides
benzoic acid + agmatine
-
172038
Fusarium anguioides
?
bleomycin B2 + H2O
-
172038
Fusarium anguioides
bleomycinic acid + agmatine
-
172038
Fusarium anguioides
?
propionyl agmatine + H2O
-
172038
Fusarium anguioides
propionic acid + agmatine
-
172038
Fusarium anguioides
?
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.22
-
benzoyl agmatine
-
Fusarium anguioides
0.8
-
bleomycin B2
-
Fusarium anguioides
1.33
-
acetyl agmatine
-
Fusarium anguioides
2
-
propionyl agmatine
-
Fusarium anguioides
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Fusarium anguioides
5829
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Fusarium anguioides
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Fusarium anguioides
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.14
-
-
Fusarium anguioides
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl agmatine + H2O
-
172038
Fusarium anguioides
acetic acid + agmatine
-
172038
Fusarium anguioides
?
benzoyl agmatine + H2O
-
172038
Fusarium anguioides
benzoic acid + agmatine
-
172038
Fusarium anguioides
?
bleomycin B2 + H2O
-
172038
Fusarium anguioides
bleomycinic acid + agmatine
-
172038
Fusarium anguioides
?
propionyl agmatine + H2O
-
172038
Fusarium anguioides
propionic acid + agmatine
-
172038
Fusarium anguioides
?
Other publictions for EC 3.5.1.40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
172037
Takahashi
Characterizations of acylagmat ...
Fusarium anguioides
J. Biochem.
77
823-830
1975
-
-
-
-
-
-
7
-
-
-
1
-
-
3
-
-
1
-
-
2
1
-
1
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
7
2
-
-
-
1
-
-
-
-
1
-
2
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
172038
Umezawa
Preparation of bleomycinic aci ...
Fusarium anguioides
J. Antibiot.
26
117-119
1973
-
-
-
-
-
-
-
4
1
-
-
-
-
2
-
-
1
-
-
1
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
1
-
1
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-