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Literature summary for 3.5.1.4 extracted from

  • Kant, B.; Kant, B.; Kumar, M.; Chand, B.
    Production and characterization of acyl transfer activity of amidase from Alcaligenes sp. MTCC 10674 for synthesis of hydroxamic acids (2013), J. Microb. Biochem. Technol., 5, 1-5.
No PubMed abstract available
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Application

Application Comment Organism
synthesis the acyl transfer activity of the enzyme can be used for the synthesis of variety of hydroxamic acids. Optimization of physiochemical parameters result into 30fold increase in the acyl transfer activity of amidase. The acyl transfer activity of amidase of Alcaligenes sp. MTCC 10674 under high temperature condition makes of potential application in developing a bioprocess for the production of variety of aliphatic and aromatic hydroxamic acid Alcaligenes sp.

Organism

Organism UniProt Comment Textmining
Alcaligenes sp.
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 isolated as a nitrile degrading bacterium from the soil
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Alcaligenes sp. MTCC 10674
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 isolated as a nitrile degrading bacterium from the soil
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the amidase of the organism exhibits dual activity i.e. hydrolase as well as acyl transfer. The acyl transfer activity of amidase forms an hydroxamate and ammonia from an amide and hydroxylamine and shows broader substrate affinity ranging from a variety of aliphatic amides (formamide, acetamide, propanamide etc.) to aromatic amides (benzamide, mandelamide, nicotinamide etc.) along with hydroxylamine for the biotransformation of these amides into corresponding hydroxamic acid, substrate specificity, overview Alcaligenes sp. ?
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 ?
additional information the amidase of the organism exhibits dual activity i.e. hydrolase as well as acyl transfer. The acyl transfer activity of amidase forms an hydroxamate and ammonia from an amide and hydroxylamine and shows broader substrate affinity ranging from a variety of aliphatic amides (formamide, acetamide, propanamide etc.) to aromatic amides (benzamide, mandelamide, nicotinamide etc.) along with hydroxylamine for the biotransformation of these amides into corresponding hydroxamic acid, substrate specificity, overview Alcaligenes sp. MTCC 10674 ?
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 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 quite stable at 50°C with t1/2 of 8 h, at 60°C t1/2 is 90 min Alcaligenes sp.