Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.4 extracted from

  • D'Abusco, A.S.; Casadio, R.; Tasco, G.; Giangiacomo, L.; Giartosio, A.; Calamia, V.; Di Marco, S.; Chiaraluce, R.; Consalvi, V.; Scandurra, R.; Politi, L.
    Oligomerization of Sulfolobus solfataricus signature amidase is promoted by acidic pH and high temperature (2005), Archaea, 1, 411-423.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
Y41C no structural modifications in the Y41C mutant, the pH-spectrum is slightly increased Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 4-hydroxybenzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
0.1
-
 2-toluamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
0.1
-
 4-hydroxybenzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
0.2
-
 cyclomaltohexaose pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
0.2
-
 4-nitrobenzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
0.2
-
 2-toluamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
0.3
-
 metacrylamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
0.3
-
 cyclomaltohexaose pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
0.3
-
 4-nitrobenzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
0.4
-
 nicotinamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
0.5
-
 metacrylamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
0.6
-
 Benzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
0.8
-
 indol-3-acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
0.8
-
 indol-3-acetamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
0.9
-
 Benzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
1.5
-
 nicotinamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
1.5
-
 Pentanamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
2.5
-
 Pentanamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
2.5
-
 3-phenylpropionamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3
-
 3-phenylpropionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.2
-
 butyramide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
4.5
-
 hexanamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
4.5
-
 hexanamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
6.5
-
 Isobutyramide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
7
-
 Isobutyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
10
-
 Propionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
10
-
 Propionamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
14
-
 butyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
22
-
 acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
37
-
 acetamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55784
-
 x * 55784, calculated from amino acid sequence Saccharolobus solfataricus
83000
-
 dimeric enzyme form, gel filtration Saccharolobus solfataricus
400000
-
 octameric enzyme form, gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus P95896
-
-
Saccharolobus solfataricus P2 P95896
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-toluamide + H2O
-
 Saccharolobus solfataricus 2-toluic acid + NH3
-
 ?
3-phenylpropionamide + H2O
-
 Saccharolobus solfataricus 3-phenylpropionate + NH3
-
 ?
4-hydroxybenzamide + H2O
-
 Saccharolobus solfataricus 4-hydroxybenzoate + NH3
-
 ?
4-methylbenzamide + H2O
-
 Saccharolobus solfataricus 4-methylbenzoic acid + NH3
-
 ?
4-nitrobenzamide + H2O
-
 Saccharolobus solfataricus 4-nitrobenzoate + NH3
-
 ?
4-toluamide + H2O
-
 Saccharolobus solfataricus 4-toluic acid + NH3
-
 ?
acetamide + H2O
-
 Saccharolobus solfataricus acetic acid + NH3
-
 ?
acetamide + H2O
-
 Saccharolobus solfataricus P2 acetic acid + NH3
-
 ?
benzamide + H2O
-
 Saccharolobus solfataricus benzoate + NH3
-
 ?
butyramide + H2O
-
 Saccharolobus solfataricus butyrate + NH3
-
 ?
butyramide + H2O
-
 Saccharolobus solfataricus P2 butyrate + NH3
-
 ?
hexanamide + H2O
-
 Saccharolobus solfataricus hexanoate + NH3
-
 ?
indole-3-acetamide + H2O
-
 Saccharolobus solfataricus indole-3-acetate + NH3
-
 ?
isobutyramide + H2O
-
 Saccharolobus solfataricus isobutyrate + NH3
-
 ?
isobutyramide + H2O
-
 Saccharolobus solfataricus P2 isobutyrate + NH3
-
 ?
methacrylamide + H2O
-
 Saccharolobus solfataricus methacrylate + NH3
-
 ?
nicotinamide + H2O
-
 Saccharolobus solfataricus nicotinic acid + NH3
-
 ?
pentanamide + H2O
-
 Saccharolobus solfataricus pentanoate + NH3
-
 ?
pentanamide + H2O
-
 Saccharolobus solfataricus P2 pentanoate + NH3
-
 ?
propionamide + H2O
-
 Saccharolobus solfataricus propionic acid + NH3
-
 ?
propionamide + H2O
-
 Saccharolobus solfataricus P2 propionic acid + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 55784, calculated from amino acid sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
signature amidase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Saccharolobus solfataricus
95
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 thermal unfolding is not reversible Saccharolobus solfataricus
70
-
 thermal stability of the wild-type enzyme and its mutant Y41C at pH 5.0 and 70 °C, are similar, with half-life values over 70 h Saccharolobus solfataricus
80
-
 half-life of wild-type enzyme: 65 h at pH 5.0, 25 h at pH 8.0. half-life of mutant enzyme Y41C: 64 h at pH 5.0, 0.5 h at pH 8.0 Saccharolobus solfataricus
84
-
 Tm-value of mutant enzyme Y41C at pH 8.0 is 83.5°C Saccharolobus solfataricus
90
-
 half-life of wild-type enzyme: 60 h at pH 5.0, 0.5 h at pH 8.0. half-life of mutant enzyme Y41C: 20 h at pH 5.0, 0.25 h at pH 8.0 Saccharolobus solfataricus
94
-
 Tm-value of wild-type enzyme at pH 8.0 is 94.4°C Saccharolobus solfataricus
97
-
 Tm-value of mutant enzyme Y41C at pH 5.0 is 97.3°C Saccharolobus solfataricus
104
-
 Tm-value of wild-type enzyme at pH 5.0 is 104.3°C Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 2-toluamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
0.8
-
 4-nitrobenzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
0.8
-
 2-toluamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
1.2
-
 indol-3-acetamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
1.9
-
 4-hydroxybenzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
2.1
-
 4-hydroxybenzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
2.2
-
 indol-3-acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
2.2
-
 nicotinamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
2.5
-
 4-nitrobenzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
3
-
 metacrylamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
4.8
-
 metacrylamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
6.4
-
 nicotinamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
6.7
-
 Benzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
7.2
-
 Benzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
12.2
-
 4-toluamide wild type enzyme Saccharolobus solfataricus
12.2
-
 4-toluamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
14.2
-
 4-toluamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
14.2
-
 4-toluamide mutant enzyme Y41C Saccharolobus solfataricus
15.3
-
 3-phenylpropionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
17.5
-
 butyramide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
18
-
 3-phenylpropionamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
19.5
-
 hexanamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
21.6
-
 hexanamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
26.8
-
 Pentanamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
28.6
-
 Pentanamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
28.9
-
 acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
30.1
-
 acetamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
34.3
-
 butyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
48.9
-
 Isobutyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
58.4
-
 Isobutyramide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
82.9
-
 Propionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
99.4
-
 Propionamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 at 70°C, assay at Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
3.5 8.5 pH 3.5: wild-type enzyme shows about 45% of maximal activity, mutant enzyme Y41C shows about 20% of maximal activity. pH 8.5: wild-type enzyme shows about 40% of maximal activity, mutant enzyme Y41C shows about 50% of maximal activity Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.81
-
 acetamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
1.31
-
 acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
1.5
-
 indol-3-acetamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
2.45
-
 butyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
2.75
-
 indol-3-acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
4
-
 2-toluamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
4
-
 4-nitrobenzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
4
-
 2-toluamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
4.26
-
 nicotinamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
4.33
-
 hexanamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
4.8
-
 hexanamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
5.1
-
 3-phenylpropionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
5.47
-
 butyramide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
5.5
-
 nicotinamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
6
-
 metacrylamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
6.99
-
 Isobutyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
7.2
-
 3-phenylpropionamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
7.44
-
 Benzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
8.29
-
 Propionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
8.33
-
 4-nitrobenzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
8.98
-
 Isobutyramide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
9.94
-
 Propionamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
10.7
-
 Pentanamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
12
-
 Benzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
16
-
 metacrylamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
19
-
 4-hydroxybenzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
19
-
 Pentanamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
21
-
 4-hydroxybenzamide pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
47.3
-
 cyclomaltohexaose pH 5.0, 70°C, mutant enzyme Y41C Saccharolobus solfataricus
61
-
 cyclomaltohexaose pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus