Cloned (Comment) | Organism |
---|---|
gene amiE, expression in Escherichia coli strain JM109 using plasmid pKK, production method evaluation and optimization, expression of amidase in soluble form and in aggregates in insoluble fractions, overview | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2R,3R)-allo-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester | - |
Pseudomonas aeruginosa | |
(2R,3S)-2-methyl-4-oxo-3-oxetanylcarbamic acid benzyl ester | - |
Pseudomonas aeruginosa | |
(2R,3S)-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester | - |
Pseudomonas aeruginosa | |
(2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid 5-phenylpentyl ester | potent inhibitor, rapidly cleaved in plasma | Pseudomonas aeruginosa | |
(2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid benzyl ester | - |
Pseudomonas aeruginosa | |
(2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester | - |
Pseudomonas aeruginosa | |
(2S,3S)-allo-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester | - |
Pseudomonas aeruginosa | |
(R)-(2-oxo-3-oxetanyl)carbamic acid tert-butyl ester | - |
Pseudomonas aeruginosa | |
(R)-1-methyl-3-(2-oxo-3-oxetanyl)urea | - |
Pseudomonas aeruginosa | |
(R)-2-oxo-3-oxetanylcarbamic acid benzyl ester | - |
Pseudomonas aeruginosa | |
(R,S)-2-oxocyclobutylcarbamic acid benzyl ester | weak inhibition | Pseudomonas aeruginosa | |
(S)-(2-oxo-3-oxetanyl)carbamic acid tert-butyl ester | - |
Pseudomonas aeruginosa | |
(S)-1-methyl-3-(2-oxo-3-oxetanyl)urea | - |
Pseudomonas aeruginosa | |
(S)-2-oxo-3-oxetanylcarbamic acid benzyl ester | - |
Pseudomonas aeruginosa | |
3-(4-phenylbutyl)oxetan-2-one | - |
Pseudomonas aeruginosa | |
6-(benzyloxy)-N-[(3S)-2-oxooxetan-3-yl]naphthalene-2-carboxamide | - |
Pseudomonas aeruginosa | |
6-methoxy-N-[(3S)-2-oxooxetan-3-yl]naphthalene-2-carboxamide | - |
Pseudomonas aeruginosa | |
cyclobutylcarbamic acid benzyl ester | weak inhibition | Pseudomonas aeruginosa | |
ethyl 6-[[(3S)-2-oxooxetan-3-yl]carbamoyl]naphthalene-2-carboxylate | - |
Pseudomonas aeruginosa | |
N-[(3S)-2-oxooxetan-3-yl]biphenyl-4-carboxamide | - |
Pseudomonas aeruginosa | |
N-[(3S)-2-oxooxetan-3-yl]naphthalene-2-carboxamide | - |
Pseudomonas aeruginosa | |
N-[(benzyloxy)carbonyl]-D-serine | weak inhibition | Pseudomonas aeruginosa | |
N-[(benzyloxy)carbonyl]-L-serine | weak inhibition | Pseudomonas aeruginosa | |
phenyl 6-[[(3S)-2-oxooxetan-3-yl]carbamoyl]naphthalene-2-carboxylate | - |
Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
gene amiE | - |
Pseudomonas aeruginosa 8602 | - |
gene amiE | - |
Renatured (Comment) | Organism |
---|---|
solubilization of amidase aggregates in insoluble fractions with up to 1.5 M, of solubilizing agent such as L-arginine or GdnHCl, followed by washing, centrifugation and dilution | Pseudomonas aeruginosa |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | formation of acetohydroxamic acid using acetamide and hydroxylamine as substrates | Pseudomonas aeruginosa | ? | - |
? | |
additional information | formation of acetohydroxamic acid using acetamide and hydroxylamine as substrates | Pseudomonas aeruginosa 8602 | ? | - |
? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000115 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | N-[(3S)-2-oxooxetan-3-yl]biphenyl-4-carboxamide | |
0.000127 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | (2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid 5-phenylpentyl ester | |
0.00019 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | (2S,3S)-allo-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester | |
0.00022 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | (2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester | |
0.0005 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | (R)-(2-oxo-3-oxetanyl)carbamic acid tert-butyl ester | |
0.00058 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | (S)-(2-oxo-3-oxetanyl)carbamic acid tert-butyl ester | |
0.0007 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | (R)-2-oxo-3-oxetanylcarbamic acid benzyl ester | |
0.001 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | (2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid benzyl ester | |
0.00296 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | (S)-2-oxo-3-oxetanylcarbamic acid benzyl ester | |
0.0049 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | (2R,3R)-allo-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester | |
0.015 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | (S)-1-methyl-3-(2-oxo-3-oxetanyl)urea | |
0.0492 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | (R)-1-methyl-3-(2-oxo-3-oxetanyl)urea | |
0.08 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | (2R,3S)-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester | |
0.1 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | N-[(benzyloxy)carbonyl]-L-serine | |
0.1 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | N-[(benzyloxy)carbonyl]-D-serine | |
0.1 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | cyclobutylcarbamic acid benzyl ester | |
0.1 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | (R,S)-2-oxocyclobutylcarbamic acid benzyl ester | |
0.1 | - |
pH 5.0, 37°C, recombinant enzyme | Pseudomonas aeruginosa | (2R,3S)-2-methyl-4-oxo-3-oxetanylcarbamic acid benzyl ester |