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Literature summary for 3.5.1.4 extracted from

  • Syed, M.; Ahmad, S.; Kusnin, N.; Shukor, M.
    Purification and characterization of amidase from acrylamide-degrading bacterium Burkholderia sp. strain DR.Y27 (2012), Afr. J. Biotechnol., 11, 329-336.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Burkholderia sp.
Al3+
-
Burkholderia sp.
As3+
-
Burkholderia sp.
Cd2+
-
Burkholderia sp.
Co2+
-
Burkholderia sp.
Cr2+
-
Burkholderia sp.
Cs+
-
Burkholderia sp.
Cu2+
-
Burkholderia sp.
Fe2+
-
Burkholderia sp.
Hg2+
-
Burkholderia sp.
Li+
-
Burkholderia sp.
Mg2+
-
Burkholderia sp.
Mn2+
-
Burkholderia sp.
additional information no inhibition by 2-mercaptoethanol and dithiotreitol; the metal ions tested show between 30% and 40% inhibition at 1 mM Burkholderia sp.
Ni2+
-
Burkholderia sp.
Pb2+
-
Burkholderia sp.
Se2+
-
Burkholderia sp.
WO42-
-
Burkholderia sp.
Zn2+
-
Burkholderia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
acetamide pH 7.5, 30°C Burkholderia sp.
0.27
-
acetamide pH 8.0, 40°C Burkholderia sp.
1.21
-
2-chloroacetamide pH 7.5, 30°C Burkholderia sp.
1.21
-
2-chloroacetamide pH 8.0, 40°C Burkholderia sp.
1.88
-
Urea pH 7.5, 30°C Burkholderia sp.
1.88
-
Urea pH 8.0, 40°C Burkholderia sp.
2.39
-
Acrylamide pH 7.5, 30°C Burkholderia sp.
2.39
-
Acrylamide pH 8.0, 40°C Burkholderia sp.
4.29
-
Propionamide pH 7.5, 30°C Burkholderia sp.
4.29
-
Propionamide pH 8.0, 40°C Burkholderia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
4 * 47000, SDS-PAGE Burkholderia sp.
186000
-
gel filtration Burkholderia sp.

Organism

Organism UniProt Comment Textmining
Burkholderia sp.
-
an acrylamide-degrading bacterium isolated from the grounds of a palm estate in Serdang, Selangor
-
Burkholderia sp.
-
previously isolated from the grounds of a palm estate in Serdang, Selangor
-
Burkholderia sp. DR.Y27
-
an acrylamide-degrading bacterium isolated from the grounds of a palm estate in Serdang, Selangor
-
Burkholderia sp. DR.Y27
-
previously isolated from the grounds of a palm estate in Serdang, Selangor
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 11.15fold to homogeneity by two steps of anion exchange chromatography, followed by gel filtration Burkholderia sp.
native enzyme 11.2fold by anion exchange chromatography, and gel filtration Burkholderia sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10
-
purified enzyme, pH 7.5, 30°C Burkholderia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloroacetamide + H2O
-
Burkholderia sp. 2-chloroacetate + NH3
-
?
acetamide + H2O
-
Burkholderia sp. acetate + NH3
-
?
acetamide + H2O best substrate Burkholderia sp. acetate + NH3
-
?
acetamide + H2O
-
Burkholderia sp. DR.Y27 acetate + NH3
-
?
acetamide + H2O best substrate Burkholderia sp. DR.Y27 acetate + NH3
-
?
acrylamide + H2O
-
Burkholderia sp. acrylate + NH3
-
?
acrylamide + H2O best substrate Burkholderia sp. acrylate + NH3
-
?
acrylamide + H2O
-
Burkholderia sp. DR.Y27 acrylate + NH3
-
?
additional information no activity with methacrylamide and nicotinamide Burkholderia sp. ?
-
?
additional information methacrylamide and nicotinamide are no substrates Burkholderia sp. ?
-
?
additional information no activity with methacrylamide and nicotinamide Burkholderia sp. DR.Y27 ?
-
?
additional information methacrylamide and nicotinamide are no substrates Burkholderia sp. DR.Y27 ?
-
?
propionamide + H2O
-
Burkholderia sp. propionate + NH3
-
?
propionamide + H2O
-
Burkholderia sp. propanoate + NH3
-
?
urea + H2O reaction of EC 3.5.1.5 Burkholderia sp. CO2 + 2 NH3
-
?
urea + H2O
-
Burkholderia sp. carbamic acid + NH3
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 47000, SDS-PAGE Burkholderia sp.
tetramer 4 * 47000, SDS-PAGE Burkholderia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Burkholderia sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 60 activity range, sharp drop in activity above 50°C Burkholderia sp.
35 60 activity range, activity decreases sharply at higher temperatures Burkholderia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Burkholderia sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.7 10.5 activity range, 61% of maximal activity at pH 5.7, 30% at pH 10.5 Burkholderia sp.
7.5 8 activity range, at pH 5.7 and pH 9.5, the enzyme loses 39% and 33% of its activity respectively, and at pH 10.5, the loss in enzyme activity is at 70% Burkholderia sp.