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Literature summary for 3.5.1.4 extracted from

  • Yue, Y.; Lian, J.; Tian, P.; Tan, T.
    Cloning of amidase gene from Rhodococcus erythropolis and expression by distinct promoters in Bacillus subtilis (2009), J. Mol. Catal. B, 56, 89-95.
No PubMed abstract available
Search for more literature for 3.5.1.4

Cloned(Commentary)

Cloned (Comment) Organism
gene ami, expression in Bacillus subtilis and in Escherichia coli strain BL21 (DE3) using different promoters. Analysis of expression effect of different promoters in Bacillus subtilis using five distinct promoters, sacB, amyE, p43, degQ, aprE, and their native signal peptide genes, to separately construct five different vectors harboring ami gene, overview. The amyE promoter along with its native signal peptide gene is most effective Rhodococcus erythropolis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for enzyme activity Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 x * 56000, recombinant enzyme, SDS-PAGE Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
-
 gene ami
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acrylamide + H2O
-
 Rhodococcus erythropolis acrylate + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, recombinant enzyme, SDS-PAGE Rhodococcus erythropolis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Rhodococcus erythropolis