Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is useful for production of optically pure S-arylpropionic acids | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
expression of gene SsAH in Escherichia coli strain BL21(DE3) as soluble, active protein, sequence comparison of wild-type and mutants enzymes, overview | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | identification of three independent SsAH mutants, SsAH-7, SsAH-14 and SsAH-15, that hydrolyze R-ketoprofen amide, although the mutations do not specifically affect residues near the active site, or directly interacting with the substrate, computational models of their three-dimensional structure, overview. Sequence comparison of wild-type and mutants enzymes, overview | Saccharolobus solfataricus |
additional information | after random mutagenesis 3 mutants are characterized | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000096 | - |
ketoprofen amide | - |
Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus | - |
gene SsAH | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
recombinant enzyme 2.35fold from Escherichia coli strain BL21(DE3) by anion exchange chromatography | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.19 | - |
>99% enantiomeric excess | Saccharolobus solfataricus |
0.52 | - |
- |
Saccharolobus solfataricus |
16 | - |
purified recombinant wild-type enzyme | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-2-(3-benzoylphenyl)propanamide + H2O | enantioselective for the S-enantiomer | Saccharolobus solfataricus | (S)-2-(3-benzoylphenyl)propanoic acid + NH3 | - |
? | |
(R,S)-ketoprofen amide + H2O | the enzyme hydrolyzes S-ketoprofen amide to its corresponding acid in an absolutely enantioselective manner | Saccharolobus solfataricus | (S)-ketoprofen + NH3 | - |
? | |
(S)-2-(3-benzoylphenyl)propanamide + H2O | ketoprofen amide, R-amide is not converted | Saccharolobus solfataricus | (S)-2-(3-benzoylphenyl)propanoic acid + NH3 | ketoprofen | ? | |
(S)-ibuprofen amide + H2O | - |
Saccharolobus solfataricus | (S)-ibuprofen + NH3 | - |
? | |
benzamide + H2O | - |
Saccharolobus solfataricus | benzoic acid + NH3 | - |
? | |
ibuprofen amide + H2O | - |
Saccharolobus solfataricus | ibuprofen + NH3 | - |
? | |
additional information | the amidase shows high enantioselectivity for (S)-arylpropionic amides, it is active on nitriles, molecular modelling, overview | Saccharolobus solfataricus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
amidase | - |
Saccharolobus solfataricus |
signature amidase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
887 | - |
ketoprofen amide | - |
Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharolobus solfataricus |