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Literature summary for 3.5.1.4 extracted from

  • Kohyama, E.; Dohi, M.; Yoshimura, A.; Yoshida, T.; Nagasawa, T.
    Remaining acetamide in acetonitrile degradation using nitrile hydratase- and amidase-producing microorganisms (2007), Appl. Microbiol. Biotechnol., 74, 829-835.
    View publication on PubMed
Search for more literature for 3.5.1.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
31.2
-
 acetamide
-
 Rhodococcus sp.
39.2
-
 acetic acid
-
 Rhodococcus sp.
1078
-
 NH3
-
 Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-
Rhodococcus sp. S13-4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
187
-
-
 Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetamide + H2O amidase-catalyzed amide formation may cause the remaining of acetamide in the acetonitrile conversion process Rhodococcus sp. acetic acid + NH3
-
 r
acetamide + H2O amidase-catalyzed amide formation may cause the remaining of acetamide in the acetonitrile conversion process Rhodococcus sp. S13-4 acetic acid + NH3
-
 r
acrylamide + H2O 42% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid Rhodococcus sp. acrylic acid + NH3
-
 r
acrylamide + H2O 42% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid Rhodococcus sp. S13-4 acrylic acid + NH3
-
 r
crotonic acid + NH3 5% of the activity with acetic acid. Reaction with crotonamide is 0.3% of the activity with acetamide Rhodococcus sp. crotonamide + H2O
-
 r
formamide + H2O 99% of the activity with acetamide Rhodococcus sp. formate + NH3
-
 ?
formamide + H2O 99% of the activity with acetamide Rhodococcus sp. S13-4 formate + NH3
-
 ?
methacrylamide + H2O 17% of the activity with acetamide. The activity of the reverse reaction is 0.1% of the activity with acetic acid Rhodococcus sp. methacrylic acid + NH3
-
 ?
additional information no activity with nicotinamide or benzamide Rhodococcus sp. ?
-
 ?
additional information no activity with nicotinamide or benzamide Rhodococcus sp. S13-4 ?
-
 ?
n-butyramide + H2O 75% of the activity with acetamide. The activity of the reverse reaction is 2% of the activity with acetic acid Rhodococcus sp. n-butyric acid + NH3
-
 r
propionamide + H2O 79% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid Rhodococcus sp. propionic acid + NH3
-
 r
propionamide + H2O 79% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid Rhodococcus sp. S13-4 propionic acid + NH3
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 acetamide hydrolysis Rhodococcus sp.
45
-
 acetamide formation Rhodococcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
47.4
-
 acetate
-
 Rhodococcus sp.
305
-
 acetamide
-
 Rhodococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 acetamide hydrolysis Rhodococcus sp.
8
-
 acetamide formation Rhodococcus sp.