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Literature summary for 3.5.1.4 extracted from

  • Rajagopal, R.; Tsurusaki, K.I.; Kannangara, G.; Kuraishi, S.; Sakurai, N.
    Natural ccurrence of indoleacetamide and amidohydrolase activity in etiolated aseptically-grown squash seedlings (1994), Plant Cell Physiol., 35, 329-339.
No PubMed abstract available
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 Indole-3-acetamide
-
 Cucurbita maxima
1.2
-
 Indole-3-acetamide
-
 Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-naphthaleneacetamide + H2O Cucurbita maxima
-
 1-naphthaleneacetic acid + NH3
-
 ?
2 indole-3-acetonitrile + 3 H2O Cucurbita maxima
-
 indole-3-acetamide + indole-3-acetic acid + NH3
-
 ?
indole-3-acetamide + H2O Cucurbita maxima
-
 indole-3-acetic acid + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
 Crown gall
-
Cucurbita maxima
-
 Duch, tissues of aseptically grown squash
-

Purification (Commentary)

Purification (Comment) Organism
enriched 48fold by fractional precipitation with ammonium sulfate, Sephadex gel filtration and anion exchange chromatography Cucurbita maxima

Source Tissue

Source Tissue Comment Organism Textmining
root of raised seedling Cucurbita maxima
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000028
-
 substrate indoleacetonitrile at 30°C, activity after purification Cucurbita maxima
0.000031
-
 substrate indole-3-acetamide at 30°C, activity after purification Cucurbita maxima

Storage Stability

Storage Stability Organism
stable to freezing Cucurbita maxima
unstable even at 4°C Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthaleneacetamide + H2O
-
 Cucurbita maxima 1-naphthaleneacetic acid + NH3
-
 ?
2 indole-3-acetonitrile + 3 H2O
-
 Cucurbita maxima indole-3-acetamide + indole-3-acetic acid + NH3
-
 ?
indole-3-acetamide + H2O
-
 Cucurbita maxima indole-3-acetic acid + NH3
-
 ?
indole-3-acetamide + H2O
-
 Agrobacterium tumefaciens indole-3-acetate + NH3
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Cucurbita maxima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 37
-
 Agrobacterium tumefaciens

pH Range

pH Minimum pH Maximum Comment Organism
7 7.5
-
 Cucurbita maxima
8.5 9.5
-
 Agrobacterium tumefaciens