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Literature summary for 3.5.1.4 extracted from

  • Nawaz, M.S.; Khan, A.A.; Bhattacharayya, D.; Siitonen, P.H.; Cerniglia, C.E.
    Physical, biochemical, and immunological characterization of a thermostable amidase from Klebsiella pneumoniae NCTR 1 (1996), J. Bacteriol., 178, 2397-2401.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis detoxification of acrylamide and as biocatalysts for the industrial production of ammonium acrylate and acrylic acid Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
EDTA 10 mM, loss of amidase activity of less than 5% Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
Acrylamide
-
Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Cobalt 0.33 mol of cobalt per mol of the native enzyme, coupled plasma-atomic emission spectrometry analysis Klebsiella pneumoniae
Iron 0.33 mol of iron per mol of the native enzyme, coupled plasma-atomic emission spectrometry analysis Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
1 * 62000, gel filtration Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
acetone precipitation, DEAE-Sepharose, ammonium sulfate precipitation, yield 10% Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.69
-
-
Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acrylamide + H2O
-
Klebsiella pneumoniae acrylate + NH3
-
?

Subunits

Subunits Comment Organism
monomer 1 * 62000, gel filtration Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
at pH 7.0 Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Klebsiella pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5
-
Klebsiella pneumoniae