Application | Comment | Organism |
---|---|---|
synthesis | detoxification of acrylamide and as biocatalysts for the industrial production of ammonium acrylate and acrylic acid | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, loss of amidase activity of less than 5% | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
Acrylamide | - |
Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cobalt | 0.33 mol of cobalt per mol of the native enzyme, coupled plasma-atomic emission spectrometry analysis | Klebsiella pneumoniae | |
Iron | 0.33 mol of iron per mol of the native enzyme, coupled plasma-atomic emission spectrometry analysis | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
1 * 62000, gel filtration | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
acetone precipitation, DEAE-Sepharose, ammonium sulfate precipitation, yield 10% | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.69 | - |
- |
Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrylamide + H2O | - |
Klebsiella pneumoniae | acrylate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 62000, gel filtration | Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
at pH 7.0 | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Klebsiella pneumoniae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | - |
Klebsiella pneumoniae |