Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 0% of origin activity | Rhodococcus erythropolis | |
Cu2+ | 0% of origin activity | Rhodococcus erythropolis | |
dithiothreitol | 97% of origin activity | Rhodococcus erythropolis | |
iodoacetate | 81% of origin activity | Rhodococcus erythropolis | |
p-hydroxymercuribenzoate | 53% of origin activity | Rhodococcus erythropolis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.067 | - |
ketoprofen amide | - |
Rhodococcus erythropolis | |
0.069 | - |
phenylacetamide | - |
Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
8 * 61000, SDS-PAGE | Rhodococcus erythropolis |
480000 | - |
gel filtration | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ketoprofen amide | Rhodococcus erythropolis | - |
2-(3-benzoylphenyl)propionic acid + NH3 | - |
? | |
ketoprofen amide | Rhodococcus erythropolis MP50 | - |
2-(3-benzoylphenyl)propionic acid + NH3 | - |
? | |
phenylacetamide | Rhodococcus erythropolis | - |
phenylacetic acid + NH3 | - |
? | |
phenylacetamide | Rhodococcus erythropolis MP50 | - |
phenylacetic acid + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | - |
- |
- |
Rhodococcus erythropolis MP50 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ion-exchange chromatography, phenyl-Superose column, desalted with a Hitrap desalting column | Rhodococcus erythropolis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.18 | - |
substrate urea | Rhodococcus erythropolis |
0.67 | - |
activity in cell extract, substrate 1 mM phenylacetamide | Rhodococcus erythropolis |
0.81 | - |
activity in cell extract, substrate 1 mM ketoprofen amide | Rhodococcus erythropolis |
1.3 | - |
activity in cell extract, substrate 1 mM naproxen amide | Rhodococcus erythropolis |
4.1 | - |
activity after purification, substrate 5 mM phenylacetamide | Rhodococcus erythropolis |
4.83 | - |
substrate methacrylamide | Rhodococcus erythropolis |
6.69 | - |
substrate cyclohexanamide | Rhodococcus erythropolis |
16.39 | - |
substrate hexanamide | Rhodococcus erythropolis |
Storage Stability | Organism |
---|---|
room temperature, half-life about 2000 days with ammonium sulfate | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ketoprofen amide | - |
Rhodococcus erythropolis | 2-(3-benzoylphenyl)propionic acid + NH3 | - |
? | |
ketoprofen amide | - |
Rhodococcus erythropolis MP50 | 2-(3-benzoylphenyl)propionic acid + NH3 | - |
? | |
phenylacetamide | - |
Rhodococcus erythropolis | phenylacetic acid + NH3 | - |
? | |
phenylacetamide | - |
Rhodococcus erythropolis MP50 | phenylacetic acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 61000, SDS-PAGE | Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Rhodococcus erythropolis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 65 | - |
Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Rhodococcus erythropolis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 10 | - |
Rhodococcus erythropolis |