Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | slight activation | Rhodotorula toruloides | |
cysteine | slight activation | Rhodotorula toruloides | |
dithiothreitol | slight activation | Rhodotorula toruloides |
General Stability | Organism |
---|---|
stabilizing effect by addition of glycine and maintaining the pH to 9.0 | Rhodotorula toruloides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Hydroxy-5-nitrobenzyl bromide | 40% inhibition at 10 mM, concentration-dependent, protection against inhibition in presence of substrate | Rhodotorula toruloides | |
Hg2+ | slight inhibition | Rhodotorula toruloides | |
iodoacetamide | slight inhibition | Rhodotorula toruloides | |
N-bromosuccinimide | concentration-dependent inhibition, 40% at 10 mM, protection against inhibition in presence of substrate | Rhodotorula toruloides | |
p-chloromercuribenzoate | slight inhibition | Rhodotorula toruloides | |
Urea | 95% loss of activity in 2.25 M | Rhodotorula toruloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.43 | - |
L-asparagine | - |
Rhodotorula toruloides | |
2.4 | - |
N-glycyl-L-asparagine | - |
Rhodotorula toruloides | |
6.45 | - |
L-glutamine | - |
Rhodotorula toruloides | |
8 | - |
N-carbamoyl-L-asparagine | - |
Rhodotorula toruloides | |
10 | - |
N-acetyl-L-asparagine | - |
Rhodotorula toruloides |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasmic space | - |
Rhodotorula toruloides | 42597 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ni2+ | slightly enhanced activity | Rhodotorula toruloides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87000 | - |
2 * 87000, SDS-PAGE | Rhodotorula toruloides |
180000 | - |
gel filtration | Rhodotorula toruloides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Rhodotorula toruloides | - |
L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | Rhodotorula toruloides | one fifth of the activity on L-asparagine | L-glutamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula toruloides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography, preparative gel electrophoresis | Rhodotorula toruloides |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Rhodotorula toruloides | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2156 | - |
glutaminase activity | Rhodotorula toruloides |
10510 | - |
asparaginase activity | Rhodotorula toruloides |
Storage Stability | Organism |
---|---|
-10°C, lyophilized to dryness in the presence of 50 mM Tris, 250 mM glycine, pH 9.0, 90% activity in 3 months | Rhodotorula toruloides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-L-aspartyl hydroxylammonium sulfate + H2O | - |
Rhodotorula toruloides | L-Asp + hydroxylammonium sulfate | - |
? | |
gamma-L-glutamyl hydroxylammonium sulfate + H2O | - |
Rhodotorula toruloides | L-glutamate + hydroxylammonium sulfate | - |
? | |
L-asparagine + H2O | - |
Rhodotorula toruloides | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | not active on D-asparagine | Rhodotorula toruloides | L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | one fifth of the activity on L-asparagine | Rhodotorula toruloides | L-glutamate + NH3 | - |
? | |
N-acetyl-L-asparagine + H2O | - |
Rhodotorula toruloides | N-acetyl-L-aspartate + NH3 | - |
? | |
N-carbamoyl-L-asparagine + H2O | - |
Rhodotorula toruloides | N-carbamoyl-L-aspartate + NH3 | - |
? | |
N-glycyl-L-asparagine + H2O | 60% higher activity than on L-asparagine | Rhodotorula toruloides | N-glycyl-L-aspartate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 87000, SDS-PAGE | Rhodotorula toruloides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Rhodotorula toruloides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | optimal range for asparaginase activity | Rhodotorula toruloides |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
complete inactivation | Rhodotorula toruloides |