Literature summary for 3.5.1.30 extracted from

Shin, J.H.; Park, S.H.; Oh, Y.H.; Choi, J.W.; Lee, M.H.; Cho, J.S.; Jeong, K.J.; Joo, J.C.; Yu, J.; Park, S.J.; Lee, S.Y.
Metabolic engineering of Corynebacterium glutamicum for enhanced production of 5-aminovaleric acid (2016), Microb. Cell Fact., 15, 174 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene davA, recombinant expression in Corynebacterium glutamicum, coexpression with gene davB from Pseudomonas putida encoding lysine 2-monooxygenase (EC 1.13.12.2)	Pseudomonas putida

Protein Variants

Protein Variants	Comment	Organism
additional information	combined expression of Pseudomonas putida davB and davA genes encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively, in engineered Corynebacterium glutamicum strain BE (KCTC 12390BP) for large scale production of 5-aminovalerate (5AVA) under the control of constitutive promoters. Expression of codon-optimized N-terminally His6-tagged davA gene fused the davB gene as an operon under a strong synthetic H36 promoter from plasmid p36davAB3 in Corynebacterium glutamicum in fed-batch culture enables the most efficient production of 5AVA. In addition, production of glutaric acid, a major byproduct, is significantly reduced by employing Corynebacterium glutamicum gabT mutant as a host. Method development and optimization, overview	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5-aminopentanamide + H2O	Pseudomonas putida	-	5-aminopentanoate + NH3	-	?
5-aminopentanamide + H2O	Pseudomonas putida ATCC 12633	-	5-aminopentanoate + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	B3IVI7	-	-
Pseudomonas putida ATCC 12633	B3IVI7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-aminopentanamide + H2O	-	Pseudomonas putida	5-aminopentanoate + NH3	-	?
5-aminopentanamide + H2O	-	Pseudomonas putida ATCC 12633	5-aminopentanoate + NH3	-	?

Synonyms

Synonyms	Comment	Organism
5-aminovaleramidase	-	Pseudomonas putida
DavA	-	Pseudomonas putida
delta-aminovaleramidase	-	Pseudomonas putida

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Release 2023.1 (January 2023)