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Literature summary for 3.5.1.3 extracted from

  • Jaisson, S.; Veiga-da-Cunha, M.; Van Schaftingen, E.
    Molecular identification of omega-amidase, the enzyme that is functionally coupled with glutamine transaminases, as the putative tumor suppressor Nit2 (2009), Biochimie, 91, 1066-1071.
    View publication on PubMed
Search for more literature for 3.5.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a fusion ptotein with an N-terminal polyHis tag in Escherichia coli and purified to homogeneity on a HisTrap column Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 2-oxosuccinamate pH 7.2, 30°C Mus musculus
0.012
-
 gamma-monomethyl-2-oxoglutaramate pH 7.2, 30°C Mus musculus
0.017
-
 2-oxosuccinamate pH 8.5, 30°C Mus musculus
0.14
-
 succinamate pH 8.5, 30°C Mus musculus
0.195
-
 2-Oxoglutaramate pH 8.5, 30°C Mus musculus
0.23
-
 succinamate pH 7.2, 30°C Mus musculus
1.27
-
 Glutaramate pH 8.5, 30°C Mus musculus
1.48
-
 Glutaramate pH 7.2, 30°C Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 bioinformatic approaches are used to exploit bacterial operons to identify a putative omega-amidase by confirming this identification through characterization of the candidate protein
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, purified to homogeneity on a HisTrap column Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutaramate + H2O tested at pH 7.2 and pH 8.5 Mus musculus 2-oxoglutarate + NH3
-
 ?
2-oxosuccinamate + H2O tested at pH 7.2 and pH 8.5 Mus musculus oxaloacetate + NH3
-
 ?
gamma-monomethyl-2-oxoglutaramate + H2O tested at pH 7.2 and pH 8.5 Mus musculus gamma-monomethyl-2-oxoglutarate + NH3
-
 ?
glutaramate + H2O tested at pH 7.2 and pH 8.5 Mus musculus glutarate + NH3
-
 ?
additional information L-glutamine and L-asparagine are no substrates at pH 7.2 and pH 8.5 Mus musculus ?
-
 ?
succinamate + H2O tested at pH 7.2 and pH 8.5 Mus musculus succinate + NH3
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.87
-
 2-oxosuccinamate pH 8.5, 30°C Mus musculus
1.15
-
 2-oxosuccinamate pH 7.2, 30°C Mus musculus
2
-
 succinamate pH 7.2, 30°C Mus musculus
2.8
-
 succinamate pH 8.5, 30°C Mus musculus
4.1
-
 Glutaramate pH 7.2, 30°C Mus musculus
8.8
-
 Glutaramate pH 8.5, 30°C Mus musculus
17.5
-
 2-Oxoglutaramate pH 8.5, 30°C Mus musculus
134.5
-
 gamma-monomethyl-2-oxoglutaramate pH 7.2, 30°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 very low activity on 2-oxoglutaramte as substrate compared to assay at pH 8.5, presumably because Nit2 only acts on the linear forms of this substrate. The generation of the linear form from the predominant, cyclic form is pH-dependent, being much fatsre at pH 8.5 than at pH 7.2 Mus musculus
8.5
-
 assay at Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.8
-
 Glutaramate pH 7.2, 30°C Mus musculus
6.9
-
 Glutaramate pH 8.5, 30°C Mus musculus
8.7
-
 succinamate pH 7.2, 30°C Mus musculus
20
-
 succinamate pH 8.5, 30°C Mus musculus
51.2
-
 2-oxosuccinamate pH 8.5, 30°C Mus musculus
89.7
-
 2-Oxoglutaramate pH 8.5, 30°C Mus musculus
385
-
 2-oxosuccinamate pH 7.2, 30°C Mus musculus
11200
-
 gamma-monomethyl-2-oxoglutaramate pH 7.2, 30°C Mus musculus