Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
cytoplasm | most of omega-amidase activity is present in cytoplasm | Rattus norvegicus | 5737 | - |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
liver, high-speed sedimentation equilibrium and amino-acid analysis | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutaramate + H2O | Mus musculus | alpha-ketoglutaramate | 2-oxoglutarate + NH3 | - |
? | |
2-oxoglutaramate + H2O | Homo sapiens | alpha-ketoglutaramate | 2-oxoglutarate + NH3 | - |
? | |
2-oxoglutaramate + H2O | Rattus norvegicus | alpha-ketoglutaramate | 2-oxoglutarate + NH3 | - |
? | |
2-oxoglutaramate + H2O | Saccharomyces cerevisiae | alpha-ketoglutaramate | 2-oxoglutarate + NH3 | - |
? | |
2-oxoglutaramate + H2O | Embryophyta | alpha-ketoglutaramate | 2-oxoglutarate + NH3 | - |
? | |
additional information | Homo sapiens | enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine | ? | - |
? | |
additional information | Rattus norvegicus | enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine | ? | - |
? | |
additional information | Mus musculus | second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway | ? | - |
? | |
additional information | Homo sapiens | second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway | ? | - |
? | |
additional information | Rattus norvegicus | second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway | ? | - |
? | |
additional information | Rattus norvegicus | glutamine-transaminase-omega-amidase pathway, metabolic role: utilization of glutamine, formation of ammonia, ammoniagenesis, and utilization of certain alpha-keto acids for synthesis of corresponding amino acids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | - |
- |
- |
Embryophyta | - |
higher plants | - |
Enterococcus faecalis | - |
- |
- |
Escherichia coli | - |
- |
- |
Homo sapiens | - |
human | - |
Lactuca sativa | - |
lettuce | - |
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
contains isozymic forms, mitochondrial and cytosolic, of omega-amidase | - |
Rattus norvegicus | - |
liver, contains isozymic forms, mitochondrial and cytosolic, of omega-amidase | - |
Saccharomyces cerevisiae | - |
- |
- |
Spinacia oleracea | - |
spinach | - |
Purification (Comment) | Organism |
---|---|
liver, cytoplasm | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3 | mechanism | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
brain | - |
Homo sapiens | - |
brain | - |
Rattus norvegicus | - |
cerebrospinal fluid | - |
Homo sapiens | - |
heart | - |
Rattus norvegicus | - |
kidney | - |
Homo sapiens | - |
kidney | - |
Rattus norvegicus | - |
kidney | - |
Canis lupus familiaris | - |
leaf | - |
Spinacia oleracea | - |
leaf | - |
Lactuca sativa | - |
liver | - |
Mus musculus | - |
liver | - |
Rattus norvegicus | - |
additional information | - |
Mus musculus | - |
additional information | - |
Homo sapiens | - |
additional information | omega-amidase is widespread, detected in various rat organs | Rattus norvegicus | - |
muscle | cardiac muscle and skeletal muscle | Rattus norvegicus | - |
Novikoff hepatoma cell | - |
Rattus norvegicus | - |
pancreas | - |
Rattus norvegicus | - |
spleen | - |
Rattus norvegicus | - |
testis | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutaramate + H2O | sole substrate is the open-chain form of alpha-ketoglutaramate, not the cyclic form | Rattus norvegicus | 2-oxoglutarate + NH3 | - |
? | |
2-oxoglutaramate + H2O | alpha-ketoglutaramate | Mus musculus | 2-oxoglutarate + NH3 | - |
? | |
2-oxoglutaramate + H2O | alpha-ketoglutaramate | Escherichia coli | 2-oxoglutarate + NH3 | - |
? | |
2-oxoglutaramate + H2O | alpha-ketoglutaramate | Homo sapiens | 2-oxoglutarate + NH3 | - |
? | |
2-oxoglutaramate + H2O | alpha-ketoglutaramate | Rattus norvegicus | 2-oxoglutarate + NH3 | - |
? | |
2-oxoglutaramate + H2O | alpha-ketoglutaramate | Saccharomyces cerevisiae | 2-oxoglutarate + NH3 | - |
? | |
2-oxoglutaramate + H2O | alpha-ketoglutaramate | Spinacia oleracea | 2-oxoglutarate + NH3 | - |
? | |
2-oxoglutaramate + H2O | alpha-ketoglutaramate | Canis lupus familiaris | 2-oxoglutarate + NH3 | - |
? | |
2-oxoglutaramate + H2O | alpha-ketoglutaramate | Enterococcus faecalis | 2-oxoglutarate + NH3 | - |
? | |
2-oxoglutaramate + H2O | alpha-ketoglutaramate | Embryophyta | 2-oxoglutarate + NH3 | - |
? | |
2-oxoglutaramate + H2O | alpha-ketoglutaramate | Lactuca sativa | 2-oxoglutarate + NH3 | - |
? | |
2-oxosuccinamate + H2O | alpha-ketosuccinamate | Rattus norvegicus | 2-oxosuccinate + NH3 | - |
? | |
2-oxosuccinamate + H2O | open-chain form | Rattus norvegicus | 2-oxosuccinate + NH3 | - |
? | |
ethyl glutarate + methylamine | methylamide formation | Rattus norvegicus | ? | - |
? | |
ethyl succinate + methylamine | methylamide formation | Rattus norvegicus | ? | - |
? | |
gamma-ethyl 2-oxoglutarate + methylamine | methylamide formation | Rattus norvegicus | ? | - |
? | |
gamma-ethyl 2-oxoglutarate + methylamine | gamma-ethyl alpha-ketoglutarate | Rattus norvegicus | ? | - |
? | |
gamma-methyl 2-oxoglutarate + methylamine | methylamide formation | Rattus norvegicus | ? | - |
? | |
glutaramate + H2O | - |
Rattus norvegicus | glutarate + NH3 | - |
? | |
glutaramate + hydroxylamine | hydroxaminolysis | Rattus norvegicus | glutaryl hydroxamate + NH3 | - |
? | |
methyl glutarate + methylamine | methylamide formation | Rattus norvegicus | ? | - |
? | |
methyl succinate + methylamine | methylamide formation | Rattus norvegicus | ? | - |
? | |
additional information | no hydrolysis of asparagine and glutamine | Rattus norvegicus | ? | - |
? | |
additional information | catalyzes methylamide formation | Rattus norvegicus | ? | - |
? | |
additional information | catalyzes hydroxaminolysis and transamidation reactions with esters and amides | Rattus norvegicus | ? | - |
? | |
additional information | enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine | Homo sapiens | ? | - |
? | |
additional information | enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine | Rattus norvegicus | ? | - |
? | |
additional information | second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway | Mus musculus | ? | - |
? | |
additional information | second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway | Homo sapiens | ? | - |
? | |
additional information | second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway | Rattus norvegicus | ? | - |
? | |
additional information | glutamine-transaminase-omega-amidase pathway, metabolic role: utilization of glutamine, formation of ammonia, ammoniagenesis, and utilization of certain alpha-keto acids for synthesis of corresponding amino acids | Rattus norvegicus | ? | - |
? | |
N-methyl-2-oxoglutaramate + H2O | at a slow rate | Rattus norvegicus | 2-oxoglutarate + methylamine | - |
? | |
N-methyl-2-oxoglutaramate + H2O | N-methyl-alpha-ketoglutaramate | Rattus norvegicus | 2-oxoglutarate + methylamine | - |
? | |
succinamate + H2O | - |
Rattus norvegicus | succinic acid + NH3 | - |
? | |
succinamate + hydroxylamine | hydroxaminolysis | Rattus norvegicus | succinyl hydroxamate + NH3 | - |
r | |
succinyl hydroxamate + H2O | - |
Rattus norvegicus | succinate + hydroxylamine | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | liver, 2 * 27000-28000, two subunits may be nonidentical, high-speed sedimentation equilibrium in the presence of 7 M guanidine hydrochloride or 7 M urea containing 0.1 M 2-mercaptoethanol | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
6.5 | 9.5 | broad pH-optimum, hydrolysis of alpha-ketosuccinamate | Rattus norvegicus |
9 | - |
hydrolysis of alpha-ketoglutaramate, sharp maximum | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |