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Literature summary for 3.5.1.3 extracted from
- Rat liver omega-amidase. Purofication and properties (1971), Biochemistry, 10, 2884-2891.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis-(2-nitrobenzoic acid) | 0.02 mM: complete inhibition; Ellman's reagent | Rattus norvegicus |  |
| ethanol | 0.05 M: no inhibition | Rattus norvegicus | |
| Glutaramate | competitive inhibition, 10 mM glutaramate, hydrolysis of methyl alpha-ketoglutarate: 43% inhibition, hydrolysis of ethyl alpha-ketoglutarate: 34% inhibition | Rattus norvegicus | |
| iodoacetamide | 0.2 mM: complete inhibition | Rattus norvegicus | |
| iodoacetate | 0.2 mM: complete inhibition | Rattus norvegicus | |
| methylamine | 0.05 M methylamine: 62% inhibition | Rattus norvegicus | |
| additional information | no inhibition by 0.05 M methanol, ethanol, propanol, ethylamine, propylamine, butylamine, dimethylamine, dierhylamine and triethylamine; no inhibition by EDTA | Rattus norvegicus | |
| N-ethylmaleimide | 0.2 mM: complete inhibition | Rattus norvegicus | |
| NH4+ | 0.05 M ammonia: 72% inhibition | Rattus norvegicus | |
| p-mercuribenzoate | 0.02 mM: complete inhibition | Rattus norvegicus | |
| succinamate | competitive inhibition, 5 mM succinamate, hydrolysis of methyl alpha-ketoglutarate: 57% inhibition, hydrolysis of ethyl alpha-ketoglutarate: 42% inhibition | Rattus norvegicus | |
| sulfhydryl reagents | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic studies, criteria and data | Rattus norvegicus | |
| 0.01 | - |
2-Oxoglutaramate | true Km of omega-amidase for alpha-ketoglutaramate open-chain structure may be of the order of 0.01 mM | Rattus norvegicus | |
| 0.03 | - |
gamma-methyl 2-oxoglutarate | hydrolysis | Rattus norvegicus | |
| 0.06 | - |
gamma-ethyl 2-oxoglutarate | hydrolysis | Rattus norvegicus | |
| 0.2 | - |
succinamate | hydrolysis | Rattus norvegicus | |
| 0.7 | - |
methyl succinate | hydrolysis | Rattus norvegicus | |
| 0.8 | - |
succinyl hydroxamate | hydrolysis | Rattus norvegicus | |
| 1.8 | - |
methyl glutarate | hydrolysis | Rattus norvegicus | |
| 3.1 | - |
Glutaramate | hydrolysis | Rattus norvegicus | |
| 3.3 | - |
2-oxoglutarate | hydrolysis | Rattus norvegicus | |
| 3.6 | - |
hydroxylamine | hydroxaminolysis, substrate: methyl glutarate | Rattus norvegicus | |
| 4 | - |
hydroxylamine | hydroxaminolysis, substrate: glutaramate | Rattus norvegicus | |
| 5.4 | - |
hydroxylamine | hydroxaminolysis, substrate: succinamate | Rattus norvegicus | |
| 6.8 | - |
propyl glutarate | hydrolysis | Rattus norvegicus | |
| 8 | - |
methyl succinate | hydroxaminolysis | Rattus norvegicus | |
| 8.3 | - |
ethyl succinate | hydrolysis | Rattus norvegicus | |
| 9.9 | - |
ethyl glutarate | hydrolysis | Rattus norvegicus | |
| 17 | - |
succinamate | hydroxaminolysis | Rattus norvegicus | |
| 17 | - |
ethyl succinate | hydroxaminolysis | Rattus norvegicus | |
| 18 | - |
propyl glutarate | hydroxaminolysis | Rattus norvegicus | |
| 25 | - |
Glutaramate | hydroxaminolysis | Rattus norvegicus | |
| 31 | - |
ethyl glutarate | hydroxaminolysis | Rattus norvegicus | |
| 52 | - |
methyl glutarate | hydroxaminolysis | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect of 0.05 M NaCl, KCl, MgCl2, CaCl2, or BaCl2 | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
liver, high-speed sedimentation equilibrium and amino-acid analysis | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutaramate + H2O | Rattus norvegicus | alpha-ketoglutaramate | 2-oxoglutarate + NH3 | - |
? | |
| additional information | Rattus norvegicus | conversion of glutamine into alpha-ketoglutarate and ammonia is brought about by two distinct enzymes: glutamine transaminase and omega-amidase | ? | - |
? | |
| additional information | Rattus norvegicus | enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine | ? | - |
? | |
| additional information | Rattus norvegicus | second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3 | mechanism | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
| 11.7 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 3°C, 1 mM dithiothreitol, 2 weeks, less than 10% activity lost | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutaramate + H2O | alpha-ketoglutaramate | Rattus norvegicus | 2-oxoglutarate + NH3 | - |
? | |
| 2-oxoglutaramate + hydroxylamine | no hydroxaminolysis of alpha-ketoglutaramate at pH 8 | Rattus norvegicus | 2-oxoglutaryl hydroxamate + NH3 | - |
? | |
| 2-oxoglutaramate + methylamine | alpha-ketoglutaramate | Rattus norvegicus | ? | - |
? | |
| 2-oxoglutaramate + methylamine | transamidation | Rattus norvegicus | ? | - |
? | |
| 2-oxosuccinamate + H2O | alpha-ketosuccinamate | Rattus norvegicus | 2-oxosuccinate + NH3 | - |
? | |
| butyl glutarate + hydoxylamine | hydroxaminolysis | Rattus norvegicus | glutaramate + butanol | - |
? | |
| delta-ethyl 2-oxoglutarate + H2O | delta-ethyl alpha-ketoglutarate | Rattus norvegicus | ? | - |
? | |
| delta-methyl 2-oxoglutarate + H2O | delta-methyl alpha-ketoglutarate | Rattus norvegicus | ? | - |
? | |
| ethyl glutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| ethyl glutarate + hydoxylamine | hydroxaminolysis | Rattus norvegicus | glutaramate + ethanol | - |
? | |
| ethyl glutarate + methylamine | transamidation | Rattus norvegicus | ? | - |
? | |
| ethyl succinate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| ethyl succinate + hydoxylamine | hydroxaminolysis | Rattus norvegicus | succinamate + ethanol | - |
? | |
| ethyl succinate + methylamine | transamidation | Rattus norvegicus | ? | - |
? | |
| gamma-ethyl 2-oxoglutarate + H2O | gamma-methyl alpha-ketoglutarate | Rattus norvegicus | ? | - |
? | |
| gamma-ethyl 2-oxoglutarate + methylamine | transamidation, poor | Rattus norvegicus | ? | - |
? | |
| gamma-ethyl 2-oxoglutarate + methylamine | gamma-ethyl alpha-ketoglutarate | Rattus norvegicus | ? | - |
? | |
| gamma-methyl 2-oxoglutarate + H2O | gamma-methyl alpha-ketoglutarate | Rattus norvegicus | ? | - |
? | |
| gamma-methyl 2-oxoglutarate + methylamine | transamidation, very poor | Rattus norvegicus | ? | - |
? | |
| glutaramate + H2O | - |
Rattus norvegicus | glutarate + NH3 | - |
? | |
| glutaramate + hydroxylamine | hydroxaminolysis | Rattus norvegicus | glutaryl hydroxamate + NH3 | - |
? | |
| glutaramate + methylamine | transamidation | Rattus norvegicus | ? | - |
? | |
| glutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| methyl glutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| methyl glutarate + hydoxylamine | hydroxaminolysis | Rattus norvegicus | glutaramate + methanol | - |
? | |
| methyl glutarate + methylamine | transamidation | Rattus norvegicus | ? | - |
? | |
| methyl succinate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| methyl succinate + hydoxylamine | hydroxaminolysis | Rattus norvegicus | succinamate + methanol | - |
? | |
| methyl succinate + methylamine | transamidation | Rattus norvegicus | ? | - |
? | |
| additional information | substrate specificity | Rattus norvegicus | ? | - |
? | |
| additional information | broad specificity | Rattus norvegicus | ? | - |
? | |
| additional information | no hydrolysis of glutamine, asparagine, N-methyl-alpha-ketoglutaramate, dimethyl glutarate, or the anilides of succinate or glutarate. No hydrolysis of N-methylsuccinamate or N-ethylsuccinamate | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme has esterolytic activity | Rattus norvegicus | ? | - |
? | |
| additional information | hydrolyzes monomethyl and monoethyl esters of alpha-ketoglutarate, gamma-ester, glutarate, succinate, and p-chloro, p-methyl, and unsubstituted phenyl esters of glutarate | Rattus norvegicus | ? | - |
? | |
| additional information | hydrolyzes monomethyl and ethyl esters of alpha-ketoglutarate, delta esters, succinate, and glutarate | Rattus norvegicus | ? | - |
? | |
| additional information | catalyzes methylamide formation | Rattus norvegicus | ? | - |
? | |
| additional information | no hydroxaminolysis of delta-methyl glutamate, delta-ethyl glutamate, malonate, glutamate, glutarate, aspartate, succinate, glutamine, asparagine, succinanilate, glutaranilate, alpha-ketoglutaramate N-methylsuccinamate, and N-ethylsuccinamate at pH 8 | Rattus norvegicus | ? | - |
? | |
| additional information | hydrolyzes monomethyl and monoethyl esters of alpha-ketoglutarate, delta-ester, glutarate, and succinate, and monopropyl ester of glutarate | Rattus norvegicus | ? | - |
? | |
| additional information | catalyzes hydroxaminolysis and transamidation reactions with esters and amides | Rattus norvegicus | ? | - |
? | |
| additional information | conversion of glutamine into alpha-ketoglutarate and ammonia is brought about by two distinct enzymes: glutamine transaminase and omega-amidase | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine | Rattus norvegicus | ? | - |
? | |
| additional information | second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway | Rattus norvegicus | ? | - |
? | |
| propyl glutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| propyl glutarate + hydoxylamine | hydroxaminolysis | Rattus norvegicus | glutaramate + propanol | - |
? | |
| succinamate + H2O | - |
Rattus norvegicus | succinic acid + NH3 | - |
? | |
| succinamate + hydroxylamine | hydroxaminolysis | Rattus norvegicus | succinyl hydroxamate + NH3 | - |
r | |
| succinamate + methylamine | transamidation | Rattus norvegicus | ? | - |
? | |
| succinate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| succinyl hydroxamate + H2O | - |
Rattus norvegicus | succinate + hydroxylamine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | liver, 2 * 27000-28000, two subunits may be nonidentical, high-speed sedimentation equilibrium in the presence of 7 M guanidine hydrochloride or 7 M urea containing 0.1 M 2-mercaptoethanol | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min, 0.02 M Tris-HCl, pH 8.5, no loss of activity | Rattus norvegicus |
| 60 | - |
5 min, 70% loss of activity | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
| 8.5 | - |
assay at | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
| 4 | - |
20 min, 90% loss of activity | Rattus norvegicus |
| 5 | - |
20 min, 32% loss of activity | Rattus norvegicus |
| 6 | 10 | 20 min, 30°C, stable between, no irreversible inactivation | Rattus norvegicus |
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