Reference to 3.5.1.3; Id = 288888 - BRENDA Enzyme Database

hydroxylamine

uncompetitive activation

Rattus norvegicus

methanol

uncompetitive activation

Rattus norvegicus

2,2,2-Trifluoroethanol

more than 2 M: irreversible inactivation

Rattus norvegicus

2-Chloroethanol

more than 2 M: irreversible inactivation

Rattus norvegicus

dioxane

up to 5% dioxane: no effect on hydrolysis of ethyl alpha-keto-glutarate or glutaramate, at 10% dioxane: slight, 5-10% inhibition

Rattus norvegicus

ethanol

more than 2 M: irreversible inactivation

Rattus norvegicus

methylamine

competitve inhibition, inhibits hydroxaminolysis of glutaramate and alpha-ketoglutaramate hydrolysis

Rattus norvegicus

additional information

no inhibition by ethylamine

Rattus norvegicus

additional information

-

additional information

kinetic studies, criteria and data

Rattus norvegicus

1

-

hydroxylamine

with substrate, acyl donor: p-methylphenyl glutarate

Rattus norvegicus

2

-

Glutaramate

hydrolysis

Rattus norvegicus

2

-

p-methylphenyl glutarate

hydrolysis

Rattus norvegicus

3

-

methyl glutarate

hydrolysis

Rattus norvegicus

3.7

-

p-chlorophenyl glutarate

hydrolysis

Rattus norvegicus

3.7

-

hydroxylamine

with substrate, acyl donor: glutaramate

Rattus norvegicus

4.1

-

hydroxylamine

with substrate, acyl donor: methyl glutarate

Rattus norvegicus

8

-

p-methoxyphenyl glutarate

hydrolysis

Rattus norvegicus

8

-

phenyl glutarate

hydrolysis

Rattus norvegicus

30

-

methylamine

with substrate, acyl donor: methyl glutarate, Km for total methylamine, for free-base form of methylamine, Km is about 102 times smaller

Rattus norvegicus

43

-

methylamine

with substrate, acyl donor: glutaramate, Km for total methylamine, for free-base form of methylamine, Km is about 102 times smaller

Rattus norvegicus

55

-

methylamine

with substrate, acyl donor: p-methylphenyl glutarate, Km for total methylamine, for free-base form of methylamine, Km is about 102 times smaller

Rattus norvegicus

5000

-

methanol

with substrate, acyl donor: glutaramate

Rattus norvegicus

2-oxoglutaramate + H2O

Rattus norvegicus

alpha-ketoglutaramate

2-oxoglutarate + NH3

-

Rattus norvegicus

?

additional information

Rattus norvegicus

enzyme, in conjunction with glutamine transaminase comprises enzyme system previously designated glutaminase II, involved in metabolism of glutamine

?

-

?

2,2,2-trifluoroethanol

more than 2 M: unstable to

Rattus norvegicus

2-chloroethanol

more than 2 M: unstable to

Rattus norvegicus

Ethanol

more than 2 M: unstable to

Rattus norvegicus

Methanol

more than 2 M: stable to and uncompetitive activation

Rattus norvegicus

-

Rattus norvegicus

a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3

kinetic evidence for an acyl-enzyme intermediate

Rattus norvegicus

a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3

two-step mechanism

Rattus norvegicus

a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3

acyl-enzyme mechanism

Rattus norvegicus

a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3

catalytic sequence involves two steps and an acyl-enzyme intermediate

Rattus norvegicus

a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3

double displacement or ping-pong mechanism

Rattus norvegicus

kidney

-

Rattus norvegicus

-

liver

-

Rattus norvegicus

-

17.4

-

ethyl alpha-ketoglutarate hydrolysis

Rattus norvegicus

2-oxoglutaramate + H2O

alpha-ketoglutaramate

288888

Rattus norvegicus

2-oxoglutarate + NH3

-

288888

Rattus norvegicus

?

delta-ethyl 2-oxoglutarate + H2O

-

288888

Rattus norvegicus

?

-

?

gamma-ethyl 2-oxoglutarate + H2O

-

288888

Rattus norvegicus

?

-

?

glutaramate + H2O

-

288888

Rattus norvegicus

glutarate + NH3

-

?

glutaramate + hydroxylamine

-

288888

Rattus norvegicus

glutaryl hydroxamate + NH3

-

?

glutaramate + methanol

methanolysis

288888

Rattus norvegicus

glutarate methyl ester + hydroxylamine

-

?

glutaramate + methylamine

-

288888

Rattus norvegicus

?

-

?

glutarate + H2O

-

288888

Rattus norvegicus

?

-

?

glutarate + hydroxylamine

acyl-transfer reaction

288888

Rattus norvegicus

glutaryl hydroxamate

-

?

methyl glutarate + H2O

-

288888

Rattus norvegicus

?

-

?

methyl glutarate + hydoxylamine

-

288888

Rattus norvegicus

glutaramate + methanol

-

?

methyl glutarate + methylamine

-

288888

Rattus norvegicus

?

-

?

additional information

substrate specificity

288888

Rattus norvegicus

?

-

?

additional information

catalyzes hydrolysis and acyl-transfer reactions with amide and monoalkyl esters of alpha-ketoglutarate, glutarate, and succinate

288888

Rattus norvegicus

?

-

?

additional information

catalyzes methanolysis, no alcoholysis using ethanol, 2-chloroethanol, and 2,2,2-trifluoroethanol

288888

Rattus norvegicus

?

-

?

additional information

rate of hydrolysis is decreased proportionally to the increase in hydroxaminolysis

288888

Rattus norvegicus

?

-

?

additional information

catalyzes hydroxaminolysis and transamidation reactions with esters and amides

288888

Rattus norvegicus

?

-

?

additional information

catalyzes hydrolysis and hydroxaminolysis

288888

Rattus norvegicus

?

-

?

additional information

enzyme, in conjunction with glutamine transaminase comprises enzyme system previously designated glutaminase II, involved in metabolism of glutamine

288888

Rattus norvegicus

?

-

?

p-chlorophenyl glutarate + H2O

-

288888

Rattus norvegicus

?

-

?

p-methoxyphenyl glutarate + H2O

-

288888

Rattus norvegicus

?

-

?

p-methylphenyl glutarate + H2O

-

288888

Rattus norvegicus

?

-

?

p-methylphenyl glutarate + hydroxylamine

-

288888

Rattus norvegicus

glutaramate + 4-methylphenol

-

?

p-methylphenyl glutarate + methylamine

-

288888

Rattus norvegicus

?

-

?

phenyl glutarate + H2O

-

288888

Rattus norvegicus

?

-

?

succinate + H2O

-

288888

Rattus norvegicus

?

-

?

30

-

p-methylphenyl glutarate hydrolysis and ethyl alpha-ketoglutarate hydrolysis, assay at

Rattus norvegicus

7

-

p-methylphenyl glutarate hydrolysis, assay at

Rattus norvegicus

7.4

-

ethyl alpha-keto-glutarate hydrolysis, assay at

Rattus norvegicus

hydroxylamine

uncompetitive activation

Rattus norvegicus

methanol

uncompetitive activation

Rattus norvegicus

2,2,2-Trifluoroethanol

more than 2 M: irreversible inactivation

Rattus norvegicus

2-Chloroethanol

more than 2 M: irreversible inactivation

Rattus norvegicus

dioxane

up to 5% dioxane: no effect on hydrolysis of ethyl alpha-keto-glutarate or glutaramate, at 10% dioxane: slight, 5-10% inhibition

Rattus norvegicus

ethanol

more than 2 M: irreversible inactivation

Rattus norvegicus

methylamine

competitve inhibition, inhibits hydroxaminolysis of glutaramate and alpha-ketoglutaramate hydrolysis

Rattus norvegicus

additional information

no inhibition by ethylamine

Rattus norvegicus

additional information

-

additional information

kinetic studies, criteria and data

Rattus norvegicus

1

-

hydroxylamine

with substrate, acyl donor: p-methylphenyl glutarate

Rattus norvegicus

2

-

Glutaramate

hydrolysis

Rattus norvegicus

2

-

p-methylphenyl glutarate

hydrolysis

Rattus norvegicus

3

-

methyl glutarate

hydrolysis

Rattus norvegicus

3.7

-

p-chlorophenyl glutarate

hydrolysis

Rattus norvegicus

3.7

-

hydroxylamine

with substrate, acyl donor: glutaramate

Rattus norvegicus

4.1

-

hydroxylamine

with substrate, acyl donor: methyl glutarate

Rattus norvegicus

8

-

p-methoxyphenyl glutarate

hydrolysis

Rattus norvegicus

8

-

phenyl glutarate

hydrolysis

Rattus norvegicus

30

-

methylamine

with substrate, acyl donor: methyl glutarate, Km for total methylamine, for free-base form of methylamine, Km is about 102 times smaller

Rattus norvegicus

43

-

methylamine

with substrate, acyl donor: glutaramate, Km for total methylamine, for free-base form of methylamine, Km is about 102 times smaller

Rattus norvegicus

55

-

methylamine

with substrate, acyl donor: p-methylphenyl glutarate, Km for total methylamine, for free-base form of methylamine, Km is about 102 times smaller

Rattus norvegicus

5000

-

methanol

with substrate, acyl donor: glutaramate

Rattus norvegicus

2-oxoglutaramate + H2O

Rattus norvegicus

alpha-ketoglutaramate

2-oxoglutarate + NH3

-

Rattus norvegicus

?

additional information

Rattus norvegicus

enzyme, in conjunction with glutamine transaminase comprises enzyme system previously designated glutaminase II, involved in metabolism of glutamine

?

-

?

2,2,2-trifluoroethanol

more than 2 M: unstable to

Rattus norvegicus

2-chloroethanol

more than 2 M: unstable to

Rattus norvegicus

Ethanol

more than 2 M: unstable to

Rattus norvegicus

Methanol

more than 2 M: stable to and uncompetitive activation

Rattus norvegicus

-

Rattus norvegicus

kidney

-

Rattus norvegicus

-

liver

-

Rattus norvegicus

-

17.4

-

ethyl alpha-ketoglutarate hydrolysis

Rattus norvegicus

2-oxoglutaramate + H2O

alpha-ketoglutaramate

288888

Rattus norvegicus

2-oxoglutarate + NH3

-

288888

Rattus norvegicus

?

delta-ethyl 2-oxoglutarate + H2O

-

288888

Rattus norvegicus

?

-

?

gamma-ethyl 2-oxoglutarate + H2O

-

288888

Rattus norvegicus

?

-

?

glutaramate + H2O

-

288888

Rattus norvegicus

glutarate + NH3

-

?

glutaramate + hydroxylamine

-

288888

Rattus norvegicus

glutaryl hydroxamate + NH3

-

?

glutaramate + methanol

methanolysis

288888

Rattus norvegicus

glutarate methyl ester + hydroxylamine

-

?

glutaramate + methylamine

-

288888

Rattus norvegicus

?

-

?

glutarate + H2O

-

288888

Rattus norvegicus

?

-

?

glutarate + hydroxylamine

acyl-transfer reaction

288888

Rattus norvegicus

glutaryl hydroxamate

-

?

methyl glutarate + H2O

-

288888

Rattus norvegicus

?

-

?

methyl glutarate + hydoxylamine

-

288888

Rattus norvegicus

glutaramate + methanol

-

?

methyl glutarate + methylamine

-

288888

Rattus norvegicus

?

-

?

additional information

substrate specificity

288888

Rattus norvegicus

?

-

?

additional information

catalyzes hydrolysis and acyl-transfer reactions with amide and monoalkyl esters of alpha-ketoglutarate, glutarate, and succinate

288888

Rattus norvegicus

?

-

?

additional information

catalyzes methanolysis, no alcoholysis using ethanol, 2-chloroethanol, and 2,2,2-trifluoroethanol

288888

Rattus norvegicus

?

-

?

additional information

rate of hydrolysis is decreased proportionally to the increase in hydroxaminolysis

288888

Rattus norvegicus

?

-

?

additional information

catalyzes hydroxaminolysis and transamidation reactions with esters and amides

288888

Rattus norvegicus

?

-

?

additional information

catalyzes hydrolysis and hydroxaminolysis

288888

Rattus norvegicus

?

-

?

additional information

enzyme, in conjunction with glutamine transaminase comprises enzyme system previously designated glutaminase II, involved in metabolism of glutamine

288888

Rattus norvegicus

?

-

?

p-chlorophenyl glutarate + H2O

-

288888

Rattus norvegicus

?

-

?

p-methoxyphenyl glutarate + H2O

-

288888

Rattus norvegicus

?

-

?

p-methylphenyl glutarate + H2O

-

288888

Rattus norvegicus

?

-

?

p-methylphenyl glutarate + hydroxylamine

-

288888

Rattus norvegicus

glutaramate + 4-methylphenol

-

?

p-methylphenyl glutarate + methylamine

-

288888

Rattus norvegicus

?

-

?

phenyl glutarate + H2O

-

288888

Rattus norvegicus

?

-

?

succinate + H2O

-

288888

Rattus norvegicus

?

-

?

30

-

p-methylphenyl glutarate hydrolysis and ethyl alpha-ketoglutarate hydrolysis, assay at

Rattus norvegicus

7

-

p-methylphenyl glutarate hydrolysis, assay at

Rattus norvegicus

7.4

-

ethyl alpha-keto-glutarate hydrolysis, assay at

Rattus norvegicus

752906

Hariharan

The enzymology of 2-hydroxygl ...

Homo sapiens

Biology

6

E24

2017

-

1

-

4

-

4

-

4

1

-

1

-

4

-

4

-

1

-

1

-

1

-

755020

Ellens

Evidence that glutamine trans ...

Solanum lycopersicum, Zea mays

Phytochemistry

113

160-169

2015

-

3

-

3

2

-

3

-

18

-

3

-

12

-

3

-

3

-

3

-

3

2

-

3

-

3

-

12

-

3

-

3

-

3

734871

Zhang

Identification and characteriz ...

Arabidopsis thaliana, Arabidopsis thaliana Col

Phytochemistry

99

36-43

2014

-

1

-

3

2

-

2

-

12

-

1

-

1

-

10

1

-

1

-

1

-

3

2

-

2

-

1

-

1

-

10

1

-

1

-

1

-

721018

Cobzaru

Homologous gene clusters of ni ...

Paenarthrobacter nicotinovorans, Rhodococcus opacus, Nocardioides sp., Nocardioides sp. JS614 / ATCC BAA-499

Res. Microbiol.

162

285-291

2011

-

2

-

4

-

8

-

5

-

3

-

3

-

2

-

4

-

5

-

3

-

3

-

6

-

710840

Krasnikov

Assay and purification of omeg ...

Rattus norvegicus

Anal. Biochem.

391

144-150

2009

-

1

-

4

-

1

-

10

-

1

-

4

2

-

2

-

1

-

1

-

1

-

4

-

1

-

1

-

4

2

-

2

-

1

-

1

-

711316

Jaisson

Molecular identification of om ...

Mus musculus

Biochimie

91

1066-1071

2009

-

1

-

8

-

12

-

1

-

1

-

6

-

1

-

8

2

-

1

-

8

-

1

-

1

-

6

-

1

-

8

2

-

8

711317

Krasnikov

Identification of the putative ...

Homo sapiens, Rattus norvegicus

Biochimie

91

1072-1080

2009

-

1

-

3

-

8

4

-

7

-

2

-

3

-

4

3

6

2

-

2

-

1

-

3

-

8

4

-

2

-

3

-

4

3

2

-

2

-

1

-

288890

Makar

Glutamine transaminase K and o ...

Gallus gallus, Homo sapiens, Mus musculus, Rattus norvegicus

J. Neurochem.

62

1983-1988

1994

-

8

-

16

-

25

-

8

-

8

-

25

-

8

-

288891

Cooper

High activities of glutamine t ...

Rattus norvegicus

J. Neurochem.

61

1731-1741

1993

-

4

-

5

-

11

-

11

-

6

-

4

-

5

-

11

-

6

-

288884

Cooper

alpha-Keto acid omega-amidase ...

Saccharomyces cerevisiae, Embryophyta, Homo sapiens, Mus musculus, Rattus norvegicus

Methods Enzymol.

113

350-358

1985

-

3

-

2

8

3

5

1

2

8

-

9

1

-

1

14

2

24

1

-

3

-

2

-

3

-

2

-

8

-

3

5

1

2

8

-

1

-

1

14

2

24

1

-

3

-

2

-

288885

Calderon

omega-Amidase pathway in the d ...

Neurospora crassa

J. Bacteriol.

161

807-809

1985

-

3

-

2

-

3

-

2

-

2

-

1

-

1

-

3

-

2

-

2

-

2

-

1

-

1

-

288893

Cooper

The glutamine transaminase-ome ...

Saccharomyces cerevisiae, Canis lupus familiaris, Escherichia coli, Enterococcus faecalis, Embryophyta, Homo sapiens, Lactuca sativa, Mus musculus, Rattus norvegicus, Spinacia oleracea

CRC Crit. Rev. Biochem.

4

281-303

1977

-

3

-

1

11

-

11

-

1

-

20

-

36

1

-

1

-

3

-

1

-

3

-

1

11

-

1

-

20

-

36

1

-

1

-

3

-

1

-

288894

Cooper

The glutamine transaminase-ome ...

Homo sapiens, Rattus norvegicus

J. Neurochem.

28

771-778

1977

-

9

-

6

-

2

-

11

2

-

6

-

2

-

2

-

9

-

6

-

11

2

-

6

-

2

-

2

-

288886

Fernald

Purification and properties of ...

Bacillus subtilis, Thermus aquaticus, Bacillus subtilis 168, Thermus aquaticus YT-1

Arch. Biochem. Biophys.

153

95-104

1972

2

-

2

6

-

6

2

4

-

124

-

2

-

2

48

-

4

2

-

6

-

2

-

2

-

2

-

2

-

8

-

6

-

6

2

4

-

2

-

2

48

-

4

2

-

6

-

288887

Ramaley

Dicarboxylate omega-amidase of ...

Bacillus subtilis, Bacillus subtilis 168

Arch. Biochem. Biophys.

153

88-94

1972

3

-

1

-

3

-

102

-

1

-

1

-

4

-

1

-

3

-

2

-

3

-

1

-

1

-

4

-

1

-

288888

Hersh

Rat liver omega-amidase. Kinet ...

Rattus norvegicus

Biochemistry

11

2251-2256

1972

2

-

6

14

-

2

4

1

-

1

5

-

2

1

-

26

-

1

-

2

-

2

-

6

-

14

-

2

4

-

1

-

2

1

-

26

-

1

-

2

-

288892

Hersh

Rat liver omega-amidase. Purof ...

Rattus norvegicus

Biochemistry

10

2884-2891

1971

-

12

23

1

4

-

3

-

1

-

2

1

49

1

-

1

-

2

-

2

-

4

-

14

-

23

1

4

-

1

-

2

1

49

1

-

2

-

2

-

4

-

288889

Meister

Hydrolysis and transfer reacti ...

Rattus norvegicus

J. Biol. Chem.

215

441-460

1955

1

-

1

-

1

-

1

-

1

-

2

-

24

-

8

-

1

-

1

-

1

-

1

-

1

-

1

-

2

-

24

-

8

-

Rat liver omega-amidase. Kinetic evidence for an acyl-enzyme intermediate

Data extracted from this reference: