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Literature summary for 3.5.1.3 extracted from
- Dicarboxylate omega-amidase of Bacillus subtilis-168: evidence for a membrane-associated form (1972), Arch. Biochem. Biophys., 153, 88-94.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sodium deoxycholate | membrane-associated form | Bacillus subtilis |  |
| sodium lauryl sulfate | membrane-associated form | Bacillus subtilis | |
| Triton X-100 | membrane-associated form | Bacillus subtilis | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NH4+ | biosynthesis of omega-amidase is repressed by free ammonium ions; biosynthesis of omega-amidase strongly repressed by ammonia, 0.001 M: 98% repression, 0.005 M: complete repression, ammonia has no effect on catalytic activity: succinyl hydroxamate formation | Bacillus subtilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Bacillus subtilis | 5737 | - |
| membrane | membrane-associated, one third to one fourth of total omega-amidase | Bacillus subtilis | 16020 | - |
| membrane | not covalently bound to membrane | Bacillus subtilis | 16020 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
168, wild-type | - |
| Bacillus subtilis 168 | - |
168, wild-type | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, membrane-associated, with detergents | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.59 | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinamate + hydroxylamine | - |
Bacillus subtilis | succinyl hydroxamate + NH3 | - |
r | |
| succinamate + hydroxylamine | - |
Bacillus subtilis 168 | succinyl hydroxamate + NH3 | - |
r | |
| succinate + hydroxylamine | - |
Bacillus subtilis | succinyl hydroxamate + H2O | - |
r | |
| succinate + hydroxylamine | - |
Bacillus subtilis 168 | succinyl hydroxamate + H2O | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bacillus subtilis |
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Release 2023.1 (January 2023)
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