Cloned (Comment) | Organism |
---|---|
gene nagA, recombinant expression in Escherichia coli strain BL21(DE3) from plasmid pET30DELTASE/nagA | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Mente kinetics. The initial rate measured for NagA has a significant substrate concentration-dependent lag phase which is not influenced by 1 mM Zn2+ and not pH-dependent | Staphylococcus aureus | |
0.16 | - |
N-acetyl-D-glucosamine-6-phosphate | pH 8.0, 30°C, recombinant enzyme | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | NagA requires two Zn2+ metal ions for activity | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43200 | - |
recombinant reduced monomeric enzyme NagA, analytical gel filtration | Staphylococcus aureus |
86300 | - |
about, dimer, sequence calculation | Staphylococcus aureus |
86700 | - |
recombinant dimeric enzyme, analytical gel filtration | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-glucosamine-6-phosphate + H2O | Staphylococcus aureus | - |
D-glucosamine 6-phosphate + acetate | - |
? | |
N-acetyl-D-glucosamine-6-phosphate + H2O | Staphylococcus aureus USA300_TCH1516 | - |
D-glucosamine 6-phosphate + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Staphylococcus aureus USA300_TCH1516 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme NagA from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography, followed by gel filtration | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | performance of a coupled assay with NagB (EC 3.5.99.6) resulting in production of fructose 6-phosphate and NH3 | Staphylococcus aureus | ? | - |
? | |
additional information | performance of a coupled assay with NagB (EC 3.5.99.6) resulting in production of fructose 6-phosphate and NH3 | Staphylococcus aureus USA300_TCH1516 | ? | - |
? | |
N-acetyl-D-glucosamine-6-phosphate + H2O | - |
Staphylococcus aureus | D-glucosamine 6-phosphate + acetate | - |
? | |
N-acetyl-D-glucosamine-6-phosphate + H2O | - |
Staphylococcus aureus USA300_TCH1516 | D-glucosamine 6-phosphate + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | NagA is an asymmetric dimer in solution, analytical ultracentrifugation and small-angle X-ray scattering data | Staphylococcus aureus |
More | involvement of both subunits in binding substrate | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
NagA | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
345 | - |
N-acetyl-D-glucosamine-6-phosphate | pH 8.0, 30°C, recombinant enzyme | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
metabolism | N-acetylglucosamine-6-phosphate deacetylase (NagA) and glucosamine-6-phosphate deaminase (NagB) are branch point enzymes that direct amino sugars into different pathways | Staphylococcus aureus |
additional information | involvement of both subunits in binding substrate by NagA. NagA requires two Zn2+ metal ions for activity and displays an unusual hysteretic behaviour, which may have a role in regulating flux at this step | Staphylococcus aureus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2156.25 | - |
N-acetyl-D-glucosamine-6-phosphate | pH 8.0, 30°C, recombinant enzyme | Staphylococcus aureus |