Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
expression in Escherichia coli | Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-methylhydroxyphosphinyl-D-glucosamine 6-phosphate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
N-acetyl-D-glucosamine 6-phosphate | - |
Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn | binding to the active site | Escherichia coli | |
Zn | binding to the active site, one cation per subunit | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-glucosamine-6-phosphate + H2O | Thermotoga maritima | - |
D-glucosamine 6-phosphate + acetate | - |
? | |
N-acetyl-D-glucosamine-6-phosphate + H2O | Escherichia coli | - |
D-glucosamine 6-phosphate + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AF18 | - |
- |
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
size exclusion column and anion exchange column | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-glucosamine-6-phosphate + H2O | - |
Thermotoga maritima | D-glucosamine 6-phosphate + acetate | - |
? | |
N-acetyl-D-glucosamine-6-phosphate + H2O | - |
Escherichia coli | D-glucosamine 6-phosphate + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | determined by gel filtration chromatography, SAXS and analytical ultracentrifugation, dimer of dimers | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
N-acetyl-D-glucosamine-6-phosphate deacetylase | - |
Thermotoga maritima |
N-acetyl-D-glucosamine-6-phosphate deacetylase | - |
Escherichia coli |
NagA | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
110 | - |
N-acetyl-D-glucosamine 6-phosphate | - |
Thermotoga maritima |