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Literature summary for 3.5.1.25 extracted from

  • Hall, R.S.; Brown, S.; Fedorov, A.A.; Fedorov, E.V.; Xu, C.; Babbitt, P.C.; Almo, S.C.; Raushel, F.M.
    Structural diversity within the mononuclear and binuclear active sites of N-acetyl-D-glucosamine-6-phosphate deacetylase (2007), Biochemistry, 46, 7953-7962.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli
expression in Escherichia coli Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Thermotoga maritima
-
Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
N-methylhydroxyphosphinyl-D-glucosamine 6-phosphate
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
N-acetyl-D-glucosamine 6-phosphate
-
Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Zn binding to the active site Escherichia coli
Zn binding to the active site, one cation per subunit Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-glucosamine-6-phosphate + H2O Thermotoga maritima
-
D-glucosamine 6-phosphate + acetate
-
?
N-acetyl-D-glucosamine-6-phosphate + H2O Escherichia coli
-
D-glucosamine 6-phosphate + acetate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AF18
-
-
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli
size exclusion column and anion exchange column Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-glucosamine-6-phosphate + H2O
-
Thermotoga maritima D-glucosamine 6-phosphate + acetate
-
?
N-acetyl-D-glucosamine-6-phosphate + H2O
-
Escherichia coli D-glucosamine 6-phosphate + acetate
-
?

Subunits

Subunits Comment Organism
homotetramer determined by gel filtration chromatography, SAXS and analytical ultracentrifugation, dimer of dimers Thermotoga maritima

Synonyms

Synonyms Comment Organism
N-acetyl-D-glucosamine-6-phosphate deacetylase
-
Thermotoga maritima
N-acetyl-D-glucosamine-6-phosphate deacetylase
-
Escherichia coli
NagA
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
110
-
N-acetyl-D-glucosamine 6-phosphate
-
Thermotoga maritima