Literature summary for 3.5.1.25 extracted from

Weidanz, J.A.; Campbell, P.; Moore, D.; deLucas, L.J.; Roden, L.; Thompson, J.N.; Vezza, P.
N-Acetylglucosamine kinase and N-acetylglucosamine 6-phosphate deacetylase in normal human erythrocytes and Plasmodium falciparum (1996), Br. J. Haematol., 95, 645-653.

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Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	some reversal by MgCl2, CoCl2 restores activity and stimulates above the control value	Homo sapiens
fructose 1,6-diphosphate	72% inhibition at 0.2 mM and 90% inhibition at 0.7 mM	Homo sapiens
fructose-6-phosphate	20% inhibition at 0.3 mM, 30% inhibition at 0.9 mM	Homo sapiens
glucosamine 6-phosphate	87% inhibition at 0.4 mM, 95% inhibition at 1.2 mM	Homo sapiens
glucose 6-phosphate	55% inhibition at 0.35 mM, 80% inhibition at 1.1 mM	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.029	-	N-acetylglucosamine 6-phosphate	-	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	restores activity after treatment with EDTA and stimulates above the control value	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-acetyl-D-glucosamine 6-phosphate + H2O	Homo sapiens	pathway of N-acetylglucosamine metabolism	D-glucosamine 6-phosphate + acetate	-	?
N-acetyl-D-glucosamine 6-phosphate + H2O	Plasmodium falciparum	pathway of N-acetylglucosamine metabolism	D-glucosamine 6-phosphate + acetate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Plasmodium falciparum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
erythrocyte	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Homo sapiens

Storage Stability

Storage Stability	Organism
4°C, 10 mM Tris-HCl, pH 7.5, 25% glycerol, 2 mM CHAPS, stable for at least 2 months	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-acetyl-D-glucosamine 6-phosphate + H2O	-	Homo sapiens	D-glucosamine 6-phosphate + acetate	-	?
N-acetyl-D-glucosamine 6-phosphate + H2O	-	Plasmodium falciparum	D-glucosamine 6-phosphate + acetate	-	?
N-acetyl-D-glucosamine 6-phosphate + H2O	pathway of N-acetylglucosamine metabolism	Homo sapiens	D-glucosamine 6-phosphate + acetate	-	?
N-acetyl-D-glucosamine 6-phosphate + H2O	pathway of N-acetylglucosamine metabolism	Plasmodium falciparum	D-glucosamine 6-phosphate + acetate	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Homo sapiens

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Release 2023.1 (January 2023)