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Literature summary for 3.5.1.24 extracted from
- A continuous fluorescence assay for simple quantification of bile salt hydrolase activity in the gut microbiome (2019), Sci. Rep., 9, 1359 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Lactiplantibacillus plantarum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycocholic acid + H2O | Lactiplantibacillus plantarum | - |
cholate + glycine | - |
? |  |
| glycocholic acid + H2O | Lactiplantibacillus plantarum NCIMB 8826 | - |
cholate + glycine | - |
? | |
| glycocholic acid + H2O | Lactiplantibacillus plantarum ATCC BAA-793 | - |
cholate + glycine | - |
? | |
| taurocholic acid + H2O | Lactiplantibacillus plantarum | - |
cholate + taurine | - |
? | |
| taurocholic acid + H2O | Lactiplantibacillus plantarum NCIMB 8826 | - |
cholate + taurine | - |
? | |
| taurocholic acid + H2O | Lactiplantibacillus plantarum ATCC BAA-793 | - |
cholate + taurine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactiplantibacillus plantarum | Q06115 | - |
- |
| Lactiplantibacillus plantarum ATCC BAA-793 | Q06115 | - |
- |
| Lactiplantibacillus plantarum NCIMB 8826 | Q06115 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholate-7-amido-4-methylcoumarin-3-acetic acid + H2O | i.e. CA-AMCA, synthetic fluorogenic substrate of BSH | Lactiplantibacillus plantarum | cholate + 7-amino-4-methylcoumarin-3-acetic acid | - |
? | |
| cholate-7-amido-4-methylcoumarin-3-acetic acid + H2O | i.e. CA-AMCA, synthetic fluorogenic substrate of BSH | Lactiplantibacillus plantarum NCIMB 8826 | cholate + 7-amino-4-methylcoumarin-3-acetic acid | - |
? | |
| cholate-7-amido-4-methylcoumarin-3-acetic acid + H2O | i.e. CA-AMCA, synthetic fluorogenic substrate of BSH | Lactiplantibacillus plantarum ATCC BAA-793 | cholate + 7-amino-4-methylcoumarin-3-acetic acid | - |
? | |
| glycocholic acid + H2O | - |
Lactiplantibacillus plantarum | cholate + glycine | - |
? | |
| glycocholic acid + H2O | - |
Lactiplantibacillus plantarum NCIMB 8826 | cholate + glycine | - |
? | |
| glycocholic acid + H2O | - |
Lactiplantibacillus plantarum ATCC BAA-793 | cholate + glycine | - |
? | |
| additional information | development and evalauation of a sensitive and simple assay method for continuous monitoring of BSH activity, a continuous fluorescence assay (AMCA fluorescence) that can be used for characterization of BSH activity with purified protein, cell lysates, whole cells, and in human gut microbiome samples, overview. Continuous, non-destructive quantification of BSH activity in a human fecal microbiome sample containing recombinant BSH is demonstrated | Lactiplantibacillus plantarum | ? | - |
? | |
| additional information | development and evalauation of a sensitive and simple assay method for continuous monitoring of BSH activity, a continuous fluorescence assay (AMCA fluorescence) that can be used for characterization of BSH activity with purified protein, cell lysates, whole cells, and in human gut microbiome samples, overview. Continuous, non-destructive quantification of BSH activity in a human fecal microbiome sample containing recombinant BSH is demonstrated | Lactiplantibacillus plantarum NCIMB 8826 | ? | - |
? | |
| additional information | development and evalauation of a sensitive and simple assay method for continuous monitoring of BSH activity, a continuous fluorescence assay (AMCA fluorescence) that can be used for characterization of BSH activity with purified protein, cell lysates, whole cells, and in human gut microbiome samples, overview. Continuous, non-destructive quantification of BSH activity in a human fecal microbiome sample containing recombinant BSH is demonstrated | Lactiplantibacillus plantarum ATCC BAA-793 | ? | - |
? | |
| taurocholic acid + H2O | - |
Lactiplantibacillus plantarum | cholate + taurine | - |
? | |
| taurocholic acid + H2O | - |
Lactiplantibacillus plantarum NCIMB 8826 | cholate + taurine | - |
? | |
| taurocholic acid + H2O | - |
Lactiplantibacillus plantarum ATCC BAA-793 | cholate + taurine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bile salt hydrolase | - |
Lactiplantibacillus plantarum |
| BSH | - |
Lactiplantibacillus plantarum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Lactiplantibacillus plantarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Lactiplantibacillus plantarum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | a key mechanism by which the microbiota in mammalian gut modify bile is through deconjugation of bile salts through bile salt hydrolase (BSH) enzymatic activity, which is postulated to be a prerequisite for all further microbial metabolism. BSH activity in the gut is largely considered to be beneficial for the host | Lactiplantibacillus plantarum |
| additional information | enzymatic hydrolysis of bile salts by BSH generally relies on a N-terminal nucleophilic cysteine that attacks the amide bond of a bile salt to free the amino acid and form an intermediate covalent enzyme-bile acid complex, which is subsequently hydrolyzed, and leaves the cysteine free to participate in another catalytic cycle | Lactiplantibacillus plantarum |
| physiological function | bile salt hydrolase (BSH) activity of the gut microbiota modulates the properties of bile salts, which are produced by the liver. BSH facilitates hydrolysis of the amide bond between the bile acid and glycine/taurine. The enzyme is a key regulator of bile acid homeostasis | Lactiplantibacillus plantarum |
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