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Literature summary for 3.5.1.24 extracted from
- Molecular cloning, expression and characterization of bile salt hydrolase from Lactobacillus rhamnosus E9 strain (2017), Food Biotechnol., 31, 128-140 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene bsh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BLR(DE3), subcloning in Escherichia coli strain XL1-Blue | Lacticaseibacillus rhamnosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycochenodeoxycholic acid + H2O | Lacticaseibacillus rhamnosus | - |
chenodeoxycholate + glycine | - |
? |  |
| glycochenodeoxycholic acid + H2O | Lacticaseibacillus rhamnosus E9 | - |
chenodeoxycholate + glycine | - |
? | |
| glycocholic acid + H2O | Lacticaseibacillus rhamnosus | - |
cholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | Lacticaseibacillus rhamnosus | - |
deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | Lacticaseibacillus rhamnosus E9 | - |
deoxycholate + glycine | - |
? | |
| taurochenodeoxycholic acid + H2O | Lacticaseibacillus rhamnosus | - |
chenodeoxycholate + taurine | - |
? | |
| taurochenodeoxycholic acid + H2O | Lacticaseibacillus rhamnosus E9 | - |
chenodeoxycholate + taurine | - |
? | |
| taurocholic acid + H2O | Lacticaseibacillus rhamnosus | - |
cholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | Lacticaseibacillus rhamnosus | - |
deoxycholate + taurine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lacticaseibacillus rhamnosus | G4XR38 | - |
- |
| Lacticaseibacillus rhamnosus E9 | G4XR38 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BLR(DE3) by nickel affinity chromatography | Lacticaseibacillus rhamnosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycochenodeoxycholic acid + H2O | - |
Lacticaseibacillus rhamnosus | chenodeoxycholate + glycine | - |
? | |
| glycochenodeoxycholic acid + H2O | best substrate | Lacticaseibacillus rhamnosus | chenodeoxycholate + glycine | - |
? | |
| glycochenodeoxycholic acid + H2O | - |
Lacticaseibacillus rhamnosus E9 | chenodeoxycholate + glycine | - |
? | |
| glycochenodeoxycholic acid + H2O | best substrate | Lacticaseibacillus rhamnosus E9 | chenodeoxycholate + glycine | - |
? | |
| glycocholic acid + H2O | - |
Lacticaseibacillus rhamnosus | cholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | - |
Lacticaseibacillus rhamnosus | deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | - |
Lacticaseibacillus rhamnosus E9 | deoxycholate + glycine | - |
? | |
| additional information | the recombinant BSH enzyme exhibits the highest affinity for glychochenodeoxycholic acid and higher affinities for glycine-conjugated bile acids than for taurine-conjugated bile acids, overview | Lacticaseibacillus rhamnosus | ? | - |
? | |
| additional information | the recombinant BSH enzyme exhibits the highest affinity for glychochenodeoxycholic acid and higher affinities for glycine-conjugated bile acids than for taurine-conjugated bile acids, overview | Lacticaseibacillus rhamnosus E9 | ? | - |
? | |
| taurochenodeoxycholic acid + H2O | - |
Lacticaseibacillus rhamnosus | chenodeoxycholate + taurine | - |
? | |
| taurochenodeoxycholic acid + H2O | - |
Lacticaseibacillus rhamnosus E9 | chenodeoxycholate + taurine | - |
? | |
| taurocholic acid + H2O | - |
Lacticaseibacillus rhamnosus | cholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | - |
Lacticaseibacillus rhamnosus | deoxycholate + taurine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, about, sequence calculation, x * 37000-40000, recombinant His-tagged enzyme, SDS-PAGE | Lacticaseibacillus rhamnosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bile salt hydrolase | - |
Lacticaseibacillus rhamnosus |
| BSH | - |
Lacticaseibacillus rhamnosus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | enzyme BSH from Lactobacillus rhamnosus strain E9 belongs to the Ntn hydrolase super family, members of which are known to have conserved N-terminal cysteine residue (Cys1) in the active center. The enzyme is phylogenetically distant to the BSHs from other Lactobacillus spp. and genera but exhibits a close phylogenetic relationship with penicilling V acylase (PVA) from Bacillus sphaericus | Lacticaseibacillus rhamnosus |
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