Cloned (Comment) | Organism |
---|---|
gene ASAH1, quantitative RT-PCR enzyme expression analysis | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | ASAH1 silencing by siRNA. An increase in expression in the cell cycle inhibitor p27 is observed upon MITF inhibition by siRNA, but does not occur in these cells when they are transduced with ASAH1 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Homo sapiens | 5764 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q13510 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
501mel cell | - |
Homo sapiens | - |
melanoma cell | human melanoma biopsies reveal heterogeneous staining of ASAH1 and low ASAH1 expression at the melanoma invasive front. ASAH1 is highly expressed and active in about 60% of melanoma cells, and its expression correlates with that of MITF | Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
acid ceramidase | - |
Homo sapiens |
ASAH1 | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | the microphthalmia-associated transcription factor (MITF) drives expression of ASAH1. MITF is a transcription factor that binds to DNA to control the expression of its targets. MITF stimulates the activity of ASAH1 promoter | up |
General Information | Comment | Organism |
---|---|---|
malfunction | ASAH1 reduction stimulates cell migration to the same extent as MITF inhibition. ASAH1 silencing in different cell types results in a significant increase in FAK phosphorylation. Conversely, forced expression of ASAH1 inhibits FAK phosphorylation. ASAH1-depleted cells are positive for the senescence-associated beta-galactosidase staining, and also MITF-silenced melanoma cells display features of senescence | Homo sapiens |
metabolism | the enzyme is a target of the microphthalmia-associated transcription factor (MITF). ASAH1 controls the switch between the proliferative and invasive phenotype in melanoma cells, and MITF also is a critical regulator of switch between proliferative and invasive phenotypes promoted by melanoma plasticity. MITF is also involved in the regulation of sphingolipid metabolism | Homo sapiens |
physiological function | acid ceramidase ASAH1 is a key enzyme of sphingolipid metabolism. ASAH1 controls melanoma cell proliferation and motile features. ASAH1 acts as a rheostat of the phenotypic switch in melanoma cells. Low ASAH1 expression is associated with an invasive behavior mediated by activation of the integrin alphavbeta5-FAK signaling cascade. ASAH1 controls the switch between the proliferative and invasive phenotype in melanoma cells | Homo sapiens |