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Literature summary for 3.5.1.23 extracted from
- Zebrafish acid ceramidase expression in Pichia pastoris GS115 and biochemical characterization (2019), Int. J. Biol. Macromol., 122, 587-593 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NP-40 | activates at 0.1% v/v | Danio rerio |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene asah1b, DNA and amino acid sequence determination and analysis, recombinant overexpression of C-terminally His-tagged enzyme in Pichia pastoris strain GS115, method optimization, overview | Danio rerio |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ceramide + H2O | Danio rerio | - |
sphingosine + a fatty acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Danio rerio | Q6PH71 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | six N-glycosylation sites at amino acid positions 173, 259, 286, 301, 342, and 348, and a single O-glycosylation site at positions 344 present on the beta-subunit are determined in the zebrafish sequence. The alpha-subunit does not have any putative glycosylation site. The recombinant enzyme is glycosylated at the beta-subunit. Deglycosylation by PNGase F | Danio rerio |
| proteolytic modification | the zebrafish enzyme is subjected to proteolytic cleavage during maturation, the enzyme has autoproteolytic activity | Danio rerio |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography, ultrafiltration, and gel filtration | Danio rerio |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 12:0-ceramide + H2O | - |
Danio rerio | sphingosine + laurate | - |
? | |
| ceramide + H2O | - |
Danio rerio | sphingosine + a fatty acid | - |
? | |
| additional information | the enzyme has autoproteolytic activity | Danio rerio | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44000, about, sequence calculation, x * 50000, about, recombinant glycosylated enzyme, SDS-PAGE | Danio rerio |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acid ceramidase | - |
Danio rerio |
| asah1b | - |
Danio rerio |
| N-acylsphingosine deacylase | - |
Danio rerio |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Danio rerio |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
assay at, autoproteolytic activity | Danio rerio |
| 5.6 | - |
assay at, ceramidase activity | Danio rerio |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Danio rerio | sequence calculation | - |
5.22 |
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