Literature summary for 3.5.1.23 extracted from

  • Zhou, Y.; Lin, X.W.; Zhang, Y.R.; Huang, Y.J.; Zhang, C.H.; Yang, Q.; Li, H.Y.; Yuan, J.Q.; Cheng, J.A.; Xu, R.; Mao, C.; Zhu, Z.R.
    Identification and biochemical characterization of Laodelphax striatellus neutral ceramidase (2013), Insect Mol. Biol., 22, 366-375.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information infection with the rice strip virus or treatment with insecticides significantly increase LsnCer mRNA expression and its enzymatic activity in Laodelphax striatellus Laodelphax striatellus

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, recombinant overexpression in Trichopulsia ni High Five cell membranes via baculoviral transfection, expression of GFP-tagged enzyme Laodelphax striatellus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 20% inhibition at 5 mM Laodelphax striatellus
Zn2+ 80% inhibition at 5 mM Laodelphax striatellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79300
-
x * 79300, about, sequence calculation Laodelphax striatellus

Organism

Organism UniProt Comment Textmining
Laodelphax striatellus R4N4U2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Trichopulsia ni High Five cell membranes Laodelphax striatellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme has broad substrate specificity with preference for ceramides with a medium acyl-chain or a mono unsaturated long acyl-chain. The enzyme prefers ceramides with unsaturated acyl-chains over ceramides with saturated acyl-chains of the same length, and it does not use phytoceramides as substrates, suggesting that LsnCer is a ceramide-specific ceramidase Laodelphax striatellus ?
-
?
N-arachidoyl-D-erythro-sphingosine + H2O low activity Laodelphax striatellus arachidonate + D-erythro-sphingosine
-
?
N-hexanoyl-D-erythro-sphingosine + H2O
-
Laodelphax striatellus hexanoate + D-erythro-sphingosine
-
?
N-lauroyl-D-erythro-sphingosine + H2O best substrate Laodelphax striatellus laurate + D-erythro-sphingosine
-
?
N-lignoceroyl-D-erythro-sphingosine + H2O low activity Laodelphax striatellus lignocerate + D-erythro-sphingosine
-
?
N-nervonoyl-D-erythro-sphingosine + H2O
-
Laodelphax striatellus nervonoate + D-erythro-sphingosine
-
?
N-oleoyl-D-erythro-sphingosine + H2O
-
Laodelphax striatellus oleate + D-erythro-sphingosine
-
?
N-palmitoyl-D-erythro-sphingosine + H2O
-
Laodelphax striatellus palmitate + D-erythro-sphingosine
-
?
N-stearoyl-D-erythro-sphingosine + H2O low activity Laodelphax striatellus stearate + D-erythro-sphingosine
-
?

Subunits

Subunits Comment Organism
? x * 79300, about, sequence calculation Laodelphax striatellus

Synonyms

Synonyms Comment Organism
neutral ceramidase
-
Laodelphax striatellus
nCDase
-
Laodelphax striatellus
LsnCer
-
Laodelphax striatellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Laodelphax striatellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Laodelphax striatellus

Expression

Organism Comment Expression
Laodelphax striatellus infection with the rice strip virus or treatment with insecticides significantly increase LsnCer mRNA expression and its enzymatic activity in Laodelphax striatellus, quantitative real time PCR enzyme expression analysis up

General Information

General Information Comment Organism
physiological function LsnCer is a bona fide neutral ceramidase that may have a role in adaption of Laodelphax striatellus to environmental stresses Laodelphax striatellus