Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.23 extracted from

  • Bhabak, K.; Proksch, D.; Redmer, S.; Arenz, C.
    Novel fluorescent ceramide derivatives for probing ceramidase substrate specificity (2012), Bioorg. Med. Chem., 20, 6154-6161.
    View publication on PubMed
Search for more literature for 3.5.1.23

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in insect cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, overview Homo sapiens
0.036
-
 N-[(2S,3R,4E)-13-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-1,3-dihydroxytridec-4-en-2-yl]hexadecanamide pH 7.0, 37°C, recombinant enzyme Homo sapiens
0.082
-
 N-[(2S,3R,4E)-13-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-1,3-dihydroxytridec-4-en-2-yl]hexadecanamide pH 4.5, 37°C, recombinant enzyme Homo sapiens
0.085
-
 N-[(2S,3R,4E)-1,3-dihydroxynonadec-4-en-2-yl]-12-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]dodecanamide pH 7.0, 37°C, recombinant enzyme Homo sapiens
0.087
-
 (2R,3Z)-2-([(2E)-1-hydroxy-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodec-2-en-1-yl]amino)heptadec-3-ene-1,3-diol pH 4.5, 37°C, recombinant enzyme Homo sapiens
0.11
-
 N-[(2S,3R,4E)-1,3-dihydroxynonadec-4-en-2-yl]-12-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]dodecanamide pH 4.5, 37°C, recombinant enzyme Homo sapiens
0.19
-
 (2R,3Z)-2-([(2E)-1-hydroxy-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodec-2-en-1-yl]amino)heptadec-3-ene-1,3-diol pH 7.0, 37°C, recombinant enzyme Homo sapiens
0.193
-
 N-[(2S,3R,4E)-1,3-dihydroxy-14-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]tetradec-4-en-2-yl]hexadecanamide pH 7.0, 37°C, recombinant enzyme Homo sapiens
0.204
-
 N-[(2S,3R,4E)-1,3-dihydroxy-14-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]tetradec-4-en-2-yl]hexadecanamide pH 4.5, 37°C, recombinant enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13510
-
-
Homo sapiens Q9NR71
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from insect cells Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3Z)-2-([(2E)-1-hydroxy-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodec-2-en-1-yl]amino)heptadec-3-ene-1,3-diol + H2O 9.9% hydrolysis Homo sapiens ?
-
 ir
additional information design and synthesis of a set of fluorescently labeled ceramides as substrates for acid and neutral ceramidases, overview. Although both the dyes, (acyl-(4-nitro-2,1,3-benzoxadiazol-7-yl)aminoethyl)-ceramide and acyl-Nile red-ceramide do not have any noticeable preference for the substitution at acyl or sphingosine part in ceramide towards hydrolysis by acid ceramidase, the ceramides with acyl-substituted NBD and Sph-substituted NR dyes have been found to be a better substrate for neutral ceramidase Homo sapiens ?
-
 ?
N-[(2S,3R,4E)-1,3-dihydroxy-14-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]tetradec-4-en-2-yl]hexadecanamide + H2O 5.6% hydrolysis Homo sapiens ?
-
 ir
N-[(2S,3R,4E)-1,3-dihydroxy-14-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]tetradec-4-en-2-yl]hexadecanamide + H2O 6.2% hydrolysis Homo sapiens ?
-
 ir
N-[(2S,3R,4E)-1,3-dihydroxynonadec-4-en-2-yl]-12-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]dodecanamide + H2O 6.1% hydrolysis Homo sapiens ?
-
 ir
N-[(2S,3R,4E)-1,3-dihydroxynonadec-4-en-2-yl]-12-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]dodecanamide + H2O 63.3% hydrolysis Homo sapiens ?
-
 ir
N-[(2S,3R,4E)-13-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-1,3-dihydroxytridec-4-en-2-yl]hexadecanamide + H2O 3.9% hydrolysis Homo sapiens ?
-
 ir
N-[(2S,3R,4E)-13-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-1,3-dihydroxytridec-4-en-2-yl]hexadecanamide + H2O 6.3% hydrolysis Homo sapiens ?
-
 ir

Synonyms

Synonyms Comment Organism
aCDase
-
 Homo sapiens
acid ceramidase
-
 Homo sapiens
nCDase
-
 Homo sapiens
neutral ceramidase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 assay at Homo sapiens
7
-
 assay at Homo sapiens