Cloned (Comment) | Organism |
---|---|
transient overexpression of FLAG-tagged ACER3 in HSC-1 cells | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ceramide + H2O | Homo sapiens | - |
sphingosine + fatty acid | - |
? | |
dihydroceramide + H2O | Homo sapiens | - |
dihydrosphingosine + fatty acid | - |
? | |
phytoceramide + H2O | Homo sapiens | - |
phytosphingosine + fatty acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-eicosenoyl-ceramide + H2O | - |
Homo sapiens | sphingosine + 11-eicosenoic acid | - |
? | |
11-eicosenoyl-dihydroceramide + H2O | - |
Homo sapiens | dihydrosphingosine + 11-eicosenoic acid | - |
? | |
11-eicosenoyl-phytoceramide + H2O | - |
Homo sapiens | phytosphingosine + 11-eicosenoic acid | - |
? | |
ceramide + H2O | - |
Homo sapiens | sphingosine + fatty acid | - |
? | |
dihydroceramide + H2O | - |
Homo sapiens | dihydrosphingosine + fatty acid | - |
? | |
additional information | ACER3 hydrolyzes C18:1-, C20:1-, C20:4-ceramides, C18:1-, C20:1-, C20:4-dihydroceramides, and C18:1-, C20:1-, C20:4-phytoceramides in vitro | Homo sapiens | ? | - |
? | |
N-arachidonoyl-ceramide + H2O | - |
Homo sapiens | sphingosine + arachidonate | - |
? | |
N-arachidonoyl-dihydroceramide + H2O | - |
Homo sapiens | dihydrosphingosine + arachidonate | - |
? | |
N-arachidonoyl-phytoceramide + H2O | - |
Homo sapiens | phytosphingosine + arachidonate | - |
? | |
N-oleoyl-ceramide + H2O | - |
Homo sapiens | sphingosine + oleate | - |
? | |
N-oleoyl-dihydroceramide + H2O | - |
Homo sapiens | dihydrosphingosine + oleate | - |
? | |
N-oleoyl-phytoceramide + H2O | - |
Homo sapiens | phytosphingosine + oleate | - |
? | |
phytoceramide + H2O | - |
Homo sapiens | phytosphingosine + fatty acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACER2 | - |
Homo sapiens |
ACER3 | - |
Homo sapiens |
alkaline ceramidase 2 | - |
Homo sapiens |
alkaline ceramidase 3 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | in cells, ACER3 overexpression decreases C18:1- and C20:1-ceramides and dihydroceramides, whereas ACER3 knockdown by RNA interference in HeLa cells has the opposite effect. ACER3 knockdown inhibits cell proliferation and up-regulates the cyclin-dependent kinase inhibitor p21CIP1/WAF1. Blocking p21CIP1/WAF1 up-regulation attenuates the inhibitory effect of ACER3 knockdown on cell proliferation, suggesting that ACER3 knockdown inhibits cell proliferation because of p21CIP1/WAF1 up-regulation. ACER3 knockdown inhibits cell apoptosis in response to serum deprivation. ACER3 knockdown up-regulates the expression of ACER2, and the ACER2 up-regulation decreases non-ULC ceramide species while increasing both sphingosine and its phosphate | Homo sapiens |
physiological function | ACER3 specifically controls the hydrolysis of ceramides carrying unsaturated long acyl chains, unsaturated long-chain ceramides | Homo sapiens |