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Literature summary for 3.5.1.23 extracted from
- Role of Drosophila alkaline ceramidase (Dacer) in Drosophila development and longevity (2010), Cell. Mol. Life Sci., 67, 1477-1490.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Bac-Dacer microsomes cause a twofold increase in ceramidase activity on D-e-C6, D-e-C12, D-e-C16, D-e-C18, and D-e-C24:1-ceramide at pH 9.0 | Drosophila melanogaster |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the Dacer/brainwashing or BWA gene CG13969 localizes to the 38B2-3 region of the left arm of the second chromosome, DNA and amino acid sequence determination and analysis, functional overexpression in High Five insect cells | Drosophila melanogaster |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | insertional mutagenesis leading to enzyme inactivation, transposon location of the dacer mutant, overview. The mutation increases the levels of ceamides in both the pupal and adult stages, and increases pre-adult development time, lifespan, and anti-oxidative stress capacity, overview | Drosophila melanogaster |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound | Drosophila melanogaster | 16020 | - |
| mitochondrion | - |
Drosophila melanogaster | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Drosophila melanogaster |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, recombinant enzyme, SDS-PAGE | Drosophila melanogaster |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ceramide + H2O | Drosophila melanogaster | - |
sphingosine + fatty acid | - |
? | |
| ceramide + H2O | Drosophila melanogaster BL-18012 | - |
sphingosine + fatty acid | - |
? | |
| additional information | Drosophila melanogaster | ceramidases are a group of enzymes that catalyze the hydrolysis of ceramides to generate sphingosine, which is phosphorylated to form sphingosine 1-phosphate | ? | - |
? | |
| additional information | Drosophila melanogaster BL-18012 | ceramidases are a group of enzymes that catalyze the hydrolysis of ceramides to generate sphingosine, which is phosphorylated to form sphingosine 1-phosphate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | Q9VIP7 | gene CG13969 | - |
| Drosophila melanogaster BL-18012 | Q9VIP7 | gene CG13969 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from High Five insect cells | Drosophila melanogaster |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| midgut | high mRNA levels | Drosophila melanogaster | - |
| additional information | high mRNA levels in the pupal stage | Drosophila melanogaster | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ceramide + H2O | - |
Drosophila melanogaster | sphingosine + fatty acid | - |
? | |
| ceramide + H2O | ceramidase activity on D-e-C6, D-e-C12, D-e-C16, D-e-C18, and D-e-C24:1-ceramide | Drosophila melanogaster | sphingosine + fatty acid | - |
? | |
| ceramide + H2O | - |
Drosophila melanogaster BL-18012 | sphingosine + fatty acid | - |
? | |
| ceramide + H2O | ceramidase activity on D-e-C6, D-e-C12, D-e-C16, D-e-C18, and D-e-C24:1-ceramide | Drosophila melanogaster BL-18012 | sphingosine + fatty acid | - |
? | |
| additional information | ceramidases are a group of enzymes that catalyze the hydrolysis of ceramides to generate sphingosine, which is phosphorylated to form sphingosine 1-phosphate | Drosophila melanogaster | ? | - |
? | |
| additional information | ceramidases are a group of enzymes that catalyze the hydrolysis of ceramides to generate sphingosine, which is phosphorylated to form sphingosine 1-phosphate | Drosophila melanogaster BL-18012 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, recombinant enzyme, SDS-PAGE | Drosophila melanogaster |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Dacer | - |
Drosophila melanogaster |
| Drosophila alkaline ceramidase | - |
Drosophila melanogaster |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Drosophila melanogaster |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Drosophila melanogaster |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | assay range | Drosophila melanogaster |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | Dacer plays a role in fly development and longevity by controlling the metabolism of ceramides | Drosophila melanogaster |
| physiological function | Dacer plays a role in fly development and longevity by controlling the metabolism of ceramides. Dacer inactivation delays Drosophila pre-adult development, while Dacer inactivation increases Drosophila lifespan | Drosophila melanogaster |
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