Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, overexpression of C-terminally myc-tagged enzyme in CHOP cells and zebrafish BRF41 cells in endoplasmic reticulum and Golgi apparatus, as well as in plasma membranes | Danio rerio |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme knockout, via antisense construct using three different antisense morpholino oligonucleotides (AMOs 1, 2, and 3) that are designed based on sequences at different sites of the 5'-untranslated region, leads to an increase in the number of zebrafish embryos with severe morphological qand cellular abnormalities such as abnormal morphogenesis inhead and tail, pericardiac edema, defect of blood cell circulation, and an increase in apoptotic cells, phenotype, overview | Danio rerio |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microvillus | of absorptive epithelial cells of the intestine | Danio rerio | 5902 | - |
plasma membrane | type II integral protein with its N-terminal end anchored to the plasma membrane and C-terminal end exposed to the extracellular space | Danio rerio | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82500 | - |
x * 110000, glycosylated recombinant enzyme, SDS-PAGE, x * 82500, deglycosylated recombinant enzyme, SDS-PAGE | Danio rerio |
110000 | - |
x * 110000, glycosylated recombinant enzyme, SDS-PAGE, x * 82500, deglycosylated recombinant enzyme, SDS-PAGE | Danio rerio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | eight potential N-glycosylation sites, four potential O-glycosylation sites | Danio rerio |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Danio rerio | - |
fin | - |
Danio rerio | - |
muscle | - |
Danio rerio | - |
small intestine | - |
Danio rerio | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Danio rerio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O | - |
Danio rerio | 4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 110000, glycosylated recombinant enzyme, SDS-PAGE, x * 82500, deglycosylated recombinant enzyme, SDS-PAGE | Danio rerio |
Synonyms | Comment | Organism |
---|---|---|
neutral ceramidase | - |
Danio rerio |
znCD | - |
Danio rerio |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Danio rerio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Danio rerio |