Cloned (Comment) | Organism |
---|---|
maCER1 gene, overexpression in HeLa cells, expression in yeast cells and in COS-1 cells, sequencing | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 60% inhibition, inhibits in a dose-dependent manner | Mus musculus | |
Mn2+ | 1 mM, 10% inhibition, inhibits in a dose-dependent manner | Mus musculus | |
additional information | not inhibited by Mg2+, phytosphingosine, dihydrosphingosine | Mus musculus | |
sphingosine | product inhibition in a dose-dependent manner, IC50: 0.08 mM | Mus musculus | |
Zn2+ | 1 mM, 60% inhibition, inhibits in a dose-dependent manner | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | maCER1 is localized to the endoplasmic reticulum and contains a Golgi to ER retrieval signal sequence | Mus musculus | 5783 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slightly activates in a dose-dependent manner | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | maCER1 may play a role in regulating the levels of bioactive lipids ceramide and sphingosine-1-phosphate, as well as complex sphingolipids, maCER1 may have a specific role in skin function | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q8R4X1 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | very low expression of maCER1 in organs and tissues other than skin | Mus musculus | - |
skin | maCER1 is predominantly and highly expressed in skin | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-erythro-C12-NBD-ceramide + H2O | maCER1 has a highly restricted substrate specificity, maCER1 has a major ceramidase activity and only a very minor reverse activity | Mus musculus | D-erythro-sphingosine + 4-nitrobenzo-2-oxa-1,3-diazoyl-dodecanoic acid | - |
r | |
additional information | maCER1 may play a role in regulating the levels of bioactive lipids ceramide and sphingosine-1-phosphate, as well as complex sphingolipids, maCER1 may have a specific role in skin function | Mus musculus | ? | - |
? | |
additional information | not: D-erythro-C12-NBD-dihydroceramide, D-ribo-C12-NBD-phytoceramide, L-threo-C12-NBD-ceramide | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alkaline ceramidase | - |
Mus musculus |
maCER1 | mouse alkaline ceramidase | Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | maCER1 has a slight activity in the range | Mus musculus |
6.5 | 10.5 | maCER1 has a negligible activity at pH lower than 6.5 or higher than 10.5 | Mus musculus |
9.5 | 10 | maCER1 has a slight activity in the range | Mus musculus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.08 | - |
product inhibition in a dose-dependent manner, IC50: 0.08 mM | Mus musculus | sphingosine |