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Literature summary for 3.5.1.22 extracted from
- Pantothenases from pseudomonads produce either pantoyl lactone or pantoic acid (1988), Biochem. J., 250, 447-451.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | - |
Pseudomonas fluorescens |  |
| di-isopropylphosphofluoridate | strain PS-21 not inhibited | Pseudomonas fluorescens | |
| KCl | - |
Pseudomonas fluorescens | |
| m-aminophenylboronic acid | - |
Pseudomonas fluorescens | |
| oxalate | - |
Pseudomonas fluorescens | |
| oxaloacetate | - |
Pseudomonas fluorescens | |
| Phenylmethanesulfonylfluoride | strain PS-21 not inhibited | Pseudomonas fluorescens | |
| SO42- | only strain PS-21 | Pseudomonas fluorescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.2 | - |
pantothenic acid | PS-21, pH 8.1, 50 mM diethanolamine/H2SO4, Km-value pH independent | Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58500 | - |
4 * 58500, strain PS-21, SDS-PAGE | Pseudomonas fluorescens |
| 240000 | - |
PS-21, gel filtration | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
PS-21 | - |
| Pseudomonas fluorescens | - |
NCIB12017, earlier Pseudomonas fluorescens UK-1, isolated from sea water | - |
| Pseudomonas fluorescens PS-21 | - |
PS-21 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.7 | - |
strain PS-21 | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pantothenic acid + H2O | PS-21 | Pseudomonas fluorescens | beta-alanine + D-pantoic acid | - |
ir | |
| pantothenic acid + H2O | PS-21 | Pseudomonas fluorescens PS-21 | beta-alanine + D-pantoic acid | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 58500, strain PS-21, SDS-PAGE | Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 9.2 | strain PS-21 | Pseudomonas fluorescens |
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