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Literature summary for 3.5.1.22 extracted from
- Purification and properties of pantothenase from Pseudomonas fluorescens (1976), Biochem. J., 157, 409-413.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Pseudomonas fluorescens |
| 97400 | - |
gel filtration | Pseudomonas fluorescens |
| 100000 | - |
gel filtration | Pseudomonas fluorescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pantothenic acid + H2O | Pseudomonas fluorescens | - |
? | - |
? |  |
| pantothenic acid + H2O | Pseudomonas fluorescens P-2 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
P-2 | - |
| Pseudomonas fluorescens | - |
biotype C | - |
| Pseudomonas fluorescens | - |
NCIB12017, earlier Pseudomonas fluorescens UK-1, isolated from sea water | - |
| Pseudomonas fluorescens P-2 | - |
P-2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.2 | - |
- |
Pseudomonas fluorescens |
| 86.9 | - |
strain P-2 | Pseudomonas fluorescens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C 20 mM potassium phosphate pH 7, enzyme is made accessible to substrates by freezing and thawing four times, decrease of activity 20% per round of freezing and thawing, frozen enzyme retains activity for at least 2 years | Pseudomonas fluorescens |
| -20°C 35 mM potassium phosphate, pH 6.8 | Pseudomonas fluorescens |
| 2°C 5 mM K2SO4, 1 week, activity dimished to 50% | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pantothenic acid + H2O | - |
Pseudomonas fluorescens | beta-alanine + D-pantoyl lactone + H2O | - |
r | |
| pantothenic acid + H2O | - |
Pseudomonas fluorescens P-2 | beta-alanine + D-pantoyl lactone + H2O | - |
r | |
| pantothenic acid + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
| pantothenic acid + H2O | - |
Pseudomonas fluorescens P-2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE | Pseudomonas fluorescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | 40 | heat inactivation occurs above 28°C, inactivated at 40°C | Pseudomonas fluorescens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | below pH 5 activity rapidly decreases | Pseudomonas fluorescens |
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