BRENDA - Reference to 3.5.1.22; Id = 171982

50000

-

2 * 50000, SDS-PAGE

Pseudomonas fluorescens

97400

-

gel filtration

Pseudomonas fluorescens

100000

-

gel filtration

Pseudomonas fluorescens

pantothenic acid + H2O

Pseudomonas fluorescens

-

?

-

pantothenic acid + H2O

Pseudomonas fluorescens P-2

-

?

-

Pseudomonas fluorescens

-

biotype C; NCIB12017, earlier Pseudomonas fluorescens UK-1, isolated from sea water; P-2

-

Pseudomonas fluorescens P-2

-

P-2

-

Pseudomonas fluorescens

13.2

-

Pseudomonas fluorescens

86.9

-

strain P-2

Pseudomonas fluorescens

-20°C 20 mM potassium phosphate pH 7, enzyme is made accessible to substrates by freezing and thawing four times, decrease of activity 20% per round of freezing and thawing, frozen enzyme retains activity for at least 2 years

Pseudomonas fluorescens

-20°C 35 mM potassium phosphate, pH 6.8

Pseudomonas fluorescens

2°C 5 mM K2SO4, 1 week, activity dimished to 50%

Pseudomonas fluorescens

pantothenic acid + H2O

-

171982

Pseudomonas fluorescens

beta-alanine + D-pantoyl lactone

-

r

pantothenic acid + H2O

-

171982

Pseudomonas fluorescens P-2

beta-alanine + D-pantoyl lactone

-

r

pantothenic acid + H2O

-

171982

Pseudomonas fluorescens

?

-

pantothenic acid + H2O

-

171982

Pseudomonas fluorescens P-2

?

-

dimer

2 * 50000, SDS-PAGE

Pseudomonas fluorescens

28

40

heat inactivation occurs above 28°C, inactivated at 40°C

Pseudomonas fluorescens

5.5

10

below pH 5 activity rapidly decreases

Pseudomonas fluorescens

50000

-

2 * 50000, SDS-PAGE

Pseudomonas fluorescens

97400

-

gel filtration

Pseudomonas fluorescens

100000

-

gel filtration

Pseudomonas fluorescens

pantothenic acid + H2O

Pseudomonas fluorescens

-

?

-

pantothenic acid + H2O

Pseudomonas fluorescens P-2

-

?

-

Pseudomonas fluorescens

13.2

-

Pseudomonas fluorescens

86.9

-

strain P-2

Pseudomonas fluorescens

-20°C 20 mM potassium phosphate pH 7, enzyme is made accessible to substrates by freezing and thawing four times, decrease of activity 20% per round of freezing and thawing, frozen enzyme retains activity for at least 2 years

Pseudomonas fluorescens

-20°C 35 mM potassium phosphate, pH 6.8

Pseudomonas fluorescens

2°C 5 mM K2SO4, 1 week, activity dimished to 50%

Pseudomonas fluorescens

pantothenic acid + H2O

-

171982

Pseudomonas fluorescens

beta-alanine + D-pantoyl lactone

-

r

pantothenic acid + H2O

-

171982

Pseudomonas fluorescens P-2

beta-alanine + D-pantoyl lactone

-

r

pantothenic acid + H2O

-

171982

Pseudomonas fluorescens

?

-

pantothenic acid + H2O

-

171982

Pseudomonas fluorescens P-2

?

-

dimer

2 * 50000, SDS-PAGE

Pseudomonas fluorescens

28

40

heat inactivation occurs above 28°C, inactivated at 40°C

Pseudomonas fluorescens

5.5

10

below pH 5 activity rapidly decreases

Pseudomonas fluorescens

171985

Kalvero Airas

Pantothenases from pseudomonad ...

Pseudomonas fluorescens, Pseudomonas fluorescens PS-21

Biochem. J.

250

447-451

1988

-

8

1

-

2

-

3

-

1

-

1

-

2

1

-

1

-

8

-

1

-

2

-

1

-

1

-

2

1

-

1

-

171974

Kalvero Airas

Pantothenase-based assay of pa ...

Pseudomonas fluorescens

Methods Enzymol.

122

33-35

1986

-

1

-

4

1

-

1

-

1

-

1

-

1

2

-

1

-

1

-

4

-

1

-

1

-

1

-

1

2

-

1

-

171975

Kalvero Airas

Pantothenase-based assay of pa ...

Pseudomonas fluorescens

Anal. Biochem.

134

122-125

1983

-

7

-

1

-

1

-

1

-

1

2

-

1

-

7

-

1

-

1

-

1

2

-

1

-

171976

Kalvero Airas

Pantothenase from Pseudomonas ...

Pseudomonas fluorescens

Methods Enzymol.

62

267-275

1979

-

2

1

-

2

1

-

1

-

1

-

1

5

1

-

1

-

1

-

2

-

2

1

-

2

1

-

1

-

1

5

1

-

1

-

1

-

171977

Solberg

Microbiological assay of panto ...

Lactobacillus plantarum, Pediococcus acidilactici

Methods Enzymol.

62

201-205

1979

-

2

-

2

-

2

-

171978

Kalvero Airas

Kinetic study on the reaction ...

Pseudomonas fluorescens

Biochemistry

17

4932-4939

1978

-

2

1

-

1

-

1

-

1

-

1

-

2

-

1

-

2

-

1

-

1

-

1

-

1

-

2

-

1

-

171979

Kalvero Airas

On the partial reactivation of ...

Pseudomonas fluorescens

Biochim. Biophys. Acta

452

201-208

1976

-

7

-

1

-

2

-

1

-

2

-

1

-

7

-

1

-

1

-

2

-

1

-

171980

Kalvero Airas

Thermal inactivation of pantot ...

Pseudomonas fluorescens

Biochim. Biophys. Acta

452

193-200

1976

-

2

-

1

-

2

-

1

-

1

2

-

1

-

2

-

1

-

1

-

1

2

-

1

-

171981

Kalvero Airas

Kinetic studies on pantothenas ...

Pseudomonas fluorescens, Pseudomonas fluorescens P-2

Biochem. J.

157

415-421

1976

-

4

8

-

2

-

6

-

1

-

4

-

1

-

4

-

8

-

2

-

1

-

4

-

1

-

171982

Kalvero Airas

Purification and properties of ...

Pseudomonas fluorescens, Pseudomonas fluorescens P-2

Biochem. J.

157

409-413

1976

-

3

2

-

7

-

1

-

2

3

4

1

-

1

-

1

-

3

2

-

1

-

2

3

4

1

-

1

-

1

-

171983

Kalvero Airas

Thermal inactivation and react ...

Pseudomonas fluorescens

Biochem. J.

130

111-119

1972

-

4

-

1

-

3

-

2

-

1

-

4

-

1

-

2

-

1

-

171984

Nurmikko

The bacterial degradation of p ...

Pseudomonas fluorescens, Pseudomonas fluorescens P-2

Biochemistry

5

399-403

1966

-

3

1

-

2

-

5

-

1

-

4

-

1

-

1

-

3

-

1

-

2

-

1

-

4

-

1

-

1

-

Purification and properties of pantothenase from Pseudomonas fluorescens

Data extracted from this reference: