Data extracted from this reference:
Molecular Weight [Da]
50000
2 * 50000, SDS-PAGE
Pseudomonas fluorescens
97400
gel filtration
Pseudomonas fluorescens
100000
gel filtration
Pseudomonas fluorescens
Natural Substrates/ Products (Substrates)
pantothenic acid + H2O
Pseudomonas fluorescens
?
pantothenic acid + H2O
Pseudomonas fluorescens P-2
?
Organism
Pseudomonas fluorescens
biotype C; NCIB12017, earlier Pseudomonas fluorescens UK-1, isolated from sea water; P-2
Pseudomonas fluorescens P-2
P-2
Purification (Commentary)
Specific Activity [micromol/min/mg]
13.2
Pseudomonas fluorescens
86.9
strain P-2
Pseudomonas fluorescens
Storage Stability
-20°C 20 mM potassium phosphate pH 7, enzyme is made accessible to substrates by freezing and thawing four times, decrease of activity 20% per round of freezing and thawing, frozen enzyme retains activity for at least 2 years
Pseudomonas fluorescens
-20°C 35 mM potassium phosphate, pH 6.8
Pseudomonas fluorescens
2°C 5 mM K2SO4, 1 week, activity dimished to 50%
Pseudomonas fluorescens
Substrates and Products (Substrate)
pantothenic acid + H2O
171982
Pseudomonas fluorescens
beta-alanine + D-pantoyl lactone
r
pantothenic acid + H2O
171982
Pseudomonas fluorescens P-2
beta-alanine + D-pantoyl lactone
r
pantothenic acid + H2O
171982
Pseudomonas fluorescens
?
pantothenic acid + H2O
171982
Pseudomonas fluorescens P-2
?
Subunits
dimer
2 * 50000, SDS-PAGE
Pseudomonas fluorescens
Temperature Stability [°C]
28
40
heat inactivation occurs above 28°C, inactivated at 40°C
Pseudomonas fluorescens
pH Stability
5.5
10
below pH 5 activity rapidly decreases
Pseudomonas fluorescens
Molecular Weight [Da] (protein specific)
50000
2 * 50000, SDS-PAGE
Pseudomonas fluorescens
97400
gel filtration
Pseudomonas fluorescens
100000
gel filtration
Pseudomonas fluorescens
Natural Substrates/ Products (Substrates) (protein specific)
pantothenic acid + H2O
Pseudomonas fluorescens
?
pantothenic acid + H2O
Pseudomonas fluorescens P-2
?
Purification (Commentary) (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
13.2
Pseudomonas fluorescens
86.9
strain P-2
Pseudomonas fluorescens
Storage Stability (protein specific)
-20°C 20 mM potassium phosphate pH 7, enzyme is made accessible to substrates by freezing and thawing four times, decrease of activity 20% per round of freezing and thawing, frozen enzyme retains activity for at least 2 years
Pseudomonas fluorescens
-20°C 35 mM potassium phosphate, pH 6.8
Pseudomonas fluorescens
2°C 5 mM K2SO4, 1 week, activity dimished to 50%
Pseudomonas fluorescens
Substrates and Products (Substrate) (protein specific)
pantothenic acid + H2O
171982
Pseudomonas fluorescens
beta-alanine + D-pantoyl lactone
r
pantothenic acid + H2O
171982
Pseudomonas fluorescens P-2
beta-alanine + D-pantoyl lactone
r
pantothenic acid + H2O
171982
Pseudomonas fluorescens
?
pantothenic acid + H2O
171982
Pseudomonas fluorescens P-2
?
Subunits (protein specific)
dimer
2 * 50000, SDS-PAGE
Pseudomonas fluorescens
Temperature Stability [°C] (protein specific)
28
40
heat inactivation occurs above 28°C, inactivated at 40°C
Pseudomonas fluorescens
pH Stability (protein specific)
5.5
10
below pH 5 activity rapidly decreases
Pseudomonas fluorescens
Other publictions for EC 3.5.1.22
171985
Kalvero Airas
Pantothenases from pseudomonad ...
Pseudomonas fluorescens, Pseudomonas fluorescens PS-21
Biochem. J.
250
447-451
1988
-
-
-
-
-
-
8
1
-
-
2
-
-
3
-
-
1
-
-
-
1
-
2
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
1
-
-
2
-
-
-
-
1
-
-
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
171974
Kalvero Airas
Pantothenase-based assay of pa ...
Pseudomonas fluorescens
Methods Enzymol.
122
33-35
1986
-
1
-
-
-
-
4
1
-
-
-
1
-
1
-
-
1
-
-
-
-
1
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
1
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
171975
Kalvero Airas
Pantothenase-based assay of pa ...
Pseudomonas fluorescens
Anal. Biochem.
134
122-125
1983
-
-
-
-
-
-
7
-
-
-
-
1
-
1
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
1
-
-
-
1
-
-
-
1
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
171976
Kalvero Airas
Pantothenase from Pseudomonas ...
Pseudomonas fluorescens
Methods Enzymol.
62
267-275
1979
-
-
-
-
-
-
2
2
1
-
2
1
-
1
-
-
1
-
-
-
1
1
5
1
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
1
-
2
1
-
-
-
1
-
-
1
1
5
1
1
-
1
-
-
1
-
-
-
-
-
-
-
-
171977
Solberg
Microbiological assay of panto ...
Lactobacillus plantarum, Pediococcus acidilactici
Methods Enzymol.
62
201-205
1979
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
171978
Kalvero Airas
Kinetic study on the reaction ...
Pseudomonas fluorescens
Biochemistry
17
4932-4939
1978
-
-
-
-
-
-
2
1
-
-
-
1
-
1
-
-
1
-
-
-
1
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
1
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
171979
Kalvero Airas
On the partial reactivation of ...
Pseudomonas fluorescens
Biochim. Biophys. Acta
452
201-208
1976
-
-
-
-
-
-
7
-
-
-
-
1
-
2
-
-
1
-
-
-
-
2
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
1
-
-
-
1
-
-
-
2
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
171980
Kalvero Airas
Thermal inactivation of pantot ...
Pseudomonas fluorescens
Biochim. Biophys. Acta
452
193-200
1976
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
1
-
-
-
-
1
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
1
-
-
-
1
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
171981
Kalvero Airas
Kinetic studies on pantothenas ...
Pseudomonas fluorescens, Pseudomonas fluorescens P-2
Biochem. J.
157
415-421
1976
-
-
-
-
-
-
4
8
-
-
-
2
-
6
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
8
-
-
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
171982
Kalvero Airas
Purification and properties of ...
Pseudomonas fluorescens, Pseudomonas fluorescens P-2
Biochem. J.
157
409-413
1976
-
-
-
-
-
-
-
-
-
-
3
2
-
7
-
-
1
-
-
-
2
3
4
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
2
-
-
-
1
-
-
2
3
4
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
171983
Kalvero Airas
Thermal inactivation and react ...
Pseudomonas fluorescens
Biochem. J.
130
111-119
1972
-
-
-
-
-
-
4
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
171984
Nurmikko
The bacterial degradation of p ...
Pseudomonas fluorescens, Pseudomonas fluorescens P-2
Biochemistry
5
399-403
1966
-
-
-
-
-
-
3
1
-
-
-
2
-
5
-
-
1
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-
-
-
-
4
-
1
1
-
-
-
1
-
-
-
-
-
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-
-
-
-
-
-
-
3
-
1
-
-
-
2
-
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-
1
-
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-
-
4
-
1
-
-
-
1
-
-
-
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-
-
-
-
-