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Literature summary for 3.5.1.2 extracted from

  • Pandian, S.; Deepak, V.; Sivasubramaniam, S.; Nellaiah, H.; Sundar, K.
    Optimization and purification of anticancer enzyme L-glutaminase from Alcaligenes faecalis KLU102 (2015), Biologia, 69, 1644-1651.
No PubMed abstract available

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
x * 37000, SDS-PAGE Alcaligenes faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + H2O Alcaligenes faecalis
-
L-glutamate + NH3
-
?
L-glutamine + H2O Alcaligenes faecalis KLU102
-
L-glutamate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Alcaligenes faecalis KLU102
-
-
-

Purification (Commentary)

Purification (Comment) Organism
acetone precipitation and carboxymethyl-methacrylate column chromatography Alcaligenes faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.094
-
crude enzyme, at pH 8.6 and 37°C Alcaligenes faecalis
0.912
-
after 9.7fold purification, at pH 8.6 and 37°C Alcaligenes faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + H2O
-
Alcaligenes faecalis L-glutamate + NH3
-
?
L-glutamine + H2O
-
Alcaligenes faecalis KLU102 L-glutamate + NH3
-
?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Alcaligenes faecalis

Synonyms

Synonyms Comment Organism
L-glutaminase
-
Alcaligenes faecalis