Cloned (Comment) | Organism |
---|---|
expressed in Aspergillus oryzae strain RIB40 RP-1 | Aspergillus sojae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | the enzyme activity is inhibited by approximately 95% in the presence of 18% (w/v) NaCl | Aspergillus sojae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66400 | - |
2 * 66400, deglycosylated enzyme, SDS-PAGE | Aspergillus sojae |
110000 | - |
2 * 110000, native enzyme, SDS-PAGE | Aspergillus sojae |
259000 | - |
gel filtration | Aspergillus sojae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | Aspergillus sojae | - |
L-glutamate + NH3 | - |
? | |
additional information | Aspergillus sojae | the enzyme prefers free L-glutamine to free L-asparagine, C-terminal glutaminyl, and asparaginyl residues in peptides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus sojae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Aspergillus sojae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.3 | - |
with L-Leu-Gln-Gly as substrate, at pH 9.0 and 50°C | Aspergillus sojae |
29.9 | - |
with Cbz-Gln-Gly as substrate, at pH 9.0 and 50°C | Aspergillus sojae |
32.4 | - |
with Cbz-L-Gln as substrate, at pH 9.0 and 50°C | Aspergillus sojae |
50.6 | - |
with D-asparagine as substrate, at pH 9.0 and 50°C | Aspergillus sojae |
73.2 | - |
with L-Pro-Leu-Gly-Gln as substrate, at pH 9.0 and 50°C | Aspergillus sojae |
236.2 | - |
with L-Ala-Gln as substrate, at pH 9.0 and 50°C | Aspergillus sojae |
308.3 | - |
with Gly-Gln as substrate, at pH 9.0 and 50°C | Aspergillus sojae |
720.5 | - |
with L-glutamine as substrate, at pH 9.0 and 50°C | Aspergillus sojae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cbz-Gln-Gly + H2O | - |
Aspergillus sojae | Cbz-Glu-Gly + NH3 | - |
? | |
Cbz-L-Gln + H2O | - |
Aspergillus sojae | Cbz-L-Glu + NH3 | - |
? | |
D-asparagine + H2O | - |
Aspergillus sojae | D-aspartate + NH3 | - |
? | |
Gly-Asn + H2O | - |
Aspergillus sojae | Gly-Asp + NH3 | - |
? | |
Gly-Gln + H2O | - |
Aspergillus sojae | Gly-Glu + NH3 | - |
? | |
L-Ala-Gln + H2O | - |
Aspergillus sojae | L-Ala-L-Glu + NH3 | - |
? | |
L-Asn + H2O | - |
Aspergillus sojae | L-Asp + NH3 | - |
? | |
L-asparagine + H2O | - |
Aspergillus sojae | L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | - |
Aspergillus sojae | L-glutamate + NH3 | - |
? | |
L-Leu-Ala-Asn + H2O | - |
Aspergillus sojae | L-Leu-Ala-Asp + NH3 | - |
? | |
L-Leu-Gln-Gly + H2O | - |
Aspergillus sojae | L-Leu-Glu-Gly + NH3 | - |
? | |
L-Leu-Gly-Asn + H2O | - |
Aspergillus sojae | L-Leu-Gly-Asp + NH3 | - |
? | |
L-Pro-Leu-Gly-Asn + H2O | - |
Aspergillus sojae | L-Pro-Leu-Gly-Asp + NH3 | - |
? | |
L-Pro-Leu-Gly-Gln + H2O | - |
Aspergillus sojae | L-Pro-Leu-Gly-Glu + NH3 | - |
? | |
additional information | the enzyme prefers free L-glutamine to free L-asparagine, C-terminal glutaminyl, and asparaginyl residues in peptides | Aspergillus sojae | ? | - |
? | |
additional information | the enzyme does not show any activity against L-Glu, D-Asn, L-Gln-Gly, gamma-glutamyl hydrazide, glutathione, and L-gamma-Glu-4-nitroanilide | Aspergillus sojae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 110000, native enzyme, SDS-PAGE | Aspergillus sojae |
homodimer | 2 * 66400, deglycosylated enzyme, SDS-PAGE | Aspergillus sojae |
Synonyms | Comment | Organism |
---|---|---|
GahB | - |
Aspergillus sojae |
glutamine amidohydrolase | - |
Aspergillus sojae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus sojae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1606 | - |
L-asparagine | at pH 9.0 and 50°C | Aspergillus sojae | |
4790 | - |
Gly-Gln | at pH 9.0 and 50°C | Aspergillus sojae | |
6249 | - |
L-glutamine | at pH 9.0 and 50°C | Aspergillus sojae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Aspergillus sojae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | the enzyme is stable in the range of pH4.0-10.0 at 30°C for 16 h | Aspergillus sojae |