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Literature summary for 3.5.1.2 extracted from

  • Singh, P.; Banik, R.M.
    Biochemical characterization and antitumor study of L-glutaminase from Bacillus cereus MTCC 1305 (2013), Appl. Biochem. Biotechnol., 171, 522-531.
    View publication on PubMed
Search for more literature for 3.5.1.2

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 138.22% activity at 100 mM Bacillus cereus
cysteine 128.15% activity at 100 mM Bacillus cereus
dithiothreitol 125.13% activity at 100 mM Bacillus cereus
glutathione 129.32% activity at 100 mM Bacillus cereus
L-ascorbic acid 128.23% activity at 100 mM Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 72.59% residual activity at 100 mM Bacillus cereus
Cd2+ complete inhibition at 50 mM Bacillus cereus
Co2+ 70.53% residual activity at 100 mM Bacillus cereus
Cu2+ complete inhibition at 50 mM Bacillus cereus
Hg2+ complete inhibition at 50 mM Bacillus cereus
iodoacetamide complete inhibition at 50 mM Bacillus cereus
Mg2+ 74.29% residual activity at 100 mM Bacillus cereus
Mn2+ 76.23% residual activity at 100 mM Bacillus cereus
additional information not inhibited by EDTA Bacillus cereus
p-chloromercuribenzoic acid complete inhibition at 10 mM Bacillus cereus
Pb2+ 69.27% residual activity at 100 mM Bacillus cereus
Zn2+ 82.12% residual activity at 100 mM Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.25
-
 L-glutamine at pH 7.5 and 35°C Bacillus cereus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 139.43% activity at 100 mM Bacillus cereus
Na+ 120.35% activity at 100 mM Bacillus cereus
PO43- 143.13% activity at 100 mM Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 4 * 35000, SDS-PAGE Bacillus cereus
140000
-
 gel filtration Bacillus cereus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + H2O Bacillus cereus
-
 L-glutamate + NH3
-
 ?
L-glutamine + H2O Bacillus cereus MTCC 1305
-
 L-glutamate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus cereus MTCC 1305
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography Bacillus cereus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.502
-
 crude extract, at pH 7.5 and 35°C Bacillus cereus
7.44
-
 after 14.8fold purification, at pH 7.5 and 35°C Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glutamine + H2O 48.5% activity compared to L-glutamine Bacillus cereus D-glutamate + NH3
-
 ?
D-glutamine + H2O 48.5% activity compared to L-glutamine Bacillus cereus MTCC 1305 D-glutamate + NH3
-
 ?
L-glutamic acid + H2O 13.5% activity compared to L-glutamine Bacillus cereus ?
-
 ?
L-glutamic acid + H2O 13.5% activity compared to L-glutamine Bacillus cereus MTCC 1305 ?
-
 ?
L-glutamine + H2O
-
 Bacillus cereus L-glutamate + NH3
-
 ?
L-glutamine + H2O 100% activity Bacillus cereus L-glutamate + NH3
-
 ?
L-glutamine + H2O
-
 Bacillus cereus MTCC 1305 L-glutamate + NH3
-
 ?
L-glutamine + H2O 100% activity Bacillus cereus MTCC 1305 L-glutamate + NH3
-
 ?
additional information the enzyme does not show any relative activity against L-asparagine, D-asparagine, and L-gamma-glutamyl-4-nitroanilide Bacillus cereus ?
-
 ?
additional information the enzyme does not show any relative activity against L-asparagine, D-asparagine, and L-gamma-glutamyl-4-nitroanilide Bacillus cereus MTCC 1305 ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 35000, SDS-PAGE Bacillus cereus

Synonyms

Synonyms Comment Organism
L-glutaminase
-
 Bacillus cereus
L-glutamine amidohydrolase
-
 Bacillus cereus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Bacillus cereus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 the enzyme shows relative activity above 70% for temperature range from 30 to 45°C and inactivation of enzyme at higher temperature above 50°C Bacillus cereus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 50 the enzyme retains relative stability up to 95% from 20 to 35°C and 50% from 40 to 50°C for 30 min. The stability of enzyme decreases sharply at higher temperature Bacillus cereus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.222
-
 L-glutamine at pH 7.5 and 35°C Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Bacillus cereus

pH Range

pH Minimum pH Maximum Comment Organism
7 8 more than 70% relative activity in pH range from 7.0 to 8.0. Loss of activity is elevated at higher pH (above 8.0), and the enzyme retains 27% of optimum activity when assayed at pH 8.5 Bacillus cereus