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Literature summary for 3.5.1.2 extracted from

  • Masuo, N.; Yoshimune, K.; Ito, K.; Matsushima, K.; Koyama, Y.; Moriguchi, M.
    Micrococcus luteus K-3-type glutaminase from Aspergillus oryzae RIB40 is salt-tolerant (2005), J. Biosci. Bioeng., 100, 576-578.
    View publication on PubMed

Application

Application Comment Organism
nutrition the enzyme from Aspergillus oryzae is important in soy sauce fermentation Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
NaCl 40% inhibition of the enzyme from strain RIB40 and 90% of the enzyme from strain MA-27-IM at 2.9 M, reduces the temperature stability of the enzyme Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular enzyme from strain MA-27-IM Aspergillus oryzae
-
-
intracellular enzyme from strain MA-27-IM Aspergillus oryzae 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme from strain RIB40 is salt-tolerant, while the enzyme from strain MA-27-IM is salt-sensitive Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + H2O Aspergillus oryzae
-
L-glutamate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
strains RIB40 and MA-27-IM
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + H2O
-
Aspergillus oryzae L-glutamate + NH3
-
?
additional information no activity with glutamine and hydroxylamine as substrates, no formation of L-glutamate-gamma-monohydroxamate Aspergillus oryzae ?
-
?

Synonyms

Synonyms Comment Organism
AoGls
-
Aspergillus oryzae
Micrococcus luteus K-3-type glutaminase only from strain RIB40, not from strain MA-27-IM Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
enzyme from strain RIB40 Aspergillus oryzae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60
-
Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
30 min, pH 7.5, the enzyme from strain RIB40 is stable Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9 enzyme from strain RIB40 Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
5.5 11
-
Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 8 30 min, 40°C, enzyme from strain RIB40, most stbale at Aspergillus oryzae