Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain JM109 | Micrococcus luteus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | inhibits the enzyme slightly at 2.6 M, the enzyme is salt-tolerant, the C-terminally serine protease-cleaved enzyme fragment shows higher salt tolerance than the full-length enzyme, the N-terminal domain has abundant glutamic acid residues on its surface, which may explain its salt-tolerant mechanism | Micrococcus luteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
L-glutamine | pH 7.5, 30°C, full-length enzyme, in presence of 2.6 M NaCl | Micrococcus luteus | |
3.7 | - |
L-glutamine | pH 7.5, 30°C, protease-cleaved large enzyme fragment, in presence of 2.6 M NaCl | Micrococcus luteus | |
3.8 | - |
L-glutamine | pH 7.5, 30°C, protease-cleaved large enzyme fragment, in absence of NaCl | Micrococcus luteus | |
6.1 | - |
L-glutamine | pH 7.5, 30°C, full-length enzyme, in absence of NaCl | Micrococcus luteus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is salt-tolerant, the C-terminally serine protease-cleaved enzyme fragment shows higher salt tolerance than the full-length enzyme, the N-terminal domain has abundant glutamic acid residues on its surface, which may explain its salt-tolerant mechanism | Micrococcus luteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38500 | - |
1 * 48300, full-length enzyme, SDS-PAGE, 1 * 38500, N-terminal large enzyme fragment, after C-terminal cleavage by serine protease, SDS-PAGE | Micrococcus luteus |
38970 | - |
N-terminal large enzyme fragment comprising residues 1-368, MALDI-TOF mass spectrometry | Micrococcus luteus |
48300 | - |
1 * 48300, full-length enzyme, SDS-PAGE, 1 * 38500, N-terminal large enzyme fragment, after C-terminal cleavage by serine protease, SDS-PAGE | Micrococcus luteus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | Micrococcus luteus | - |
L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | Micrococcus luteus K-3 | - |
L-glutamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus luteus | - |
- |
- |
Micrococcus luteus K-3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from strain K-3 to homogeneity, recombinant enzyme from Escherichia coli strain JM109 by anion exchange chromatography to homogeneity | Micrococcus luteus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1230 | - |
purified native enzyme | Micrococcus luteus |
1246 | - |
purified recombinant enzyme | Micrococcus luteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | - |
Micrococcus luteus | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | - |
Micrococcus luteus K-3 | L-glutamate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 48300, full-length enzyme, SDS-PAGE, 1 * 38500, N-terminal large enzyme fragment, after C-terminal cleavage by serine protease, SDS-PAGE | Micrococcus luteus |
Synonyms | Comment | Organism |
---|---|---|
salt-tolerant glutaminase | - |
Micrococcus luteus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Micrococcus luteus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
128 | - |
L-glutamine | pH 7.5, 30°C, full-length enzyme, in presence of 2.6 M NaCl | Micrococcus luteus | |
403.3 | - |
L-glutamine | pH 7.5, 30°C, full-length enzyme, in presence of 2.6 M NaCl | Micrococcus luteus | |
821.7 | - |
L-glutamine | pH 7.5, 30°C, protease-cleaved large enzyme fragment, in presence of 2.6 M NaCl | Micrococcus luteus | |
873.3 | - |
L-glutamine | pH 7.5, 30°C, protease-cleaved large enzyme fragment, in absence of NaCl | Micrococcus luteus | |
1373 | - |
L-glutamine | pH 7.5, 30°C, full-length enzyme, in absence of NaCl | Micrococcus luteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Micrococcus luteus |