Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.19 extracted from

  • Taniguchi, H.; Sungwallek, S.; Chotchuang, P.; Okano, K.; Honda, K.
    A key enzyme of the NAD+ salvage pathway in Thermus thermophilus characterization of nicotinamidase and the impact of its gene deletion at high temperatures (2017), J. Bacteriol., 199, e00359-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.19

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 nicotinamide pH and temperature not specified in the publication Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotinamide + H2O Thermus thermophilus key enzyme of the NAD+ salvage pathway nicotinate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q5SLG5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotinamide + H2O
-
 Thermus thermophilus nicotinate + NH3
-
 ?
nicotinamide + H2O key enzyme of the NAD+ salvage pathway Thermus thermophilus nicotinate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
TTHA0328
-
 Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 80 50°C: about 75% of maximal activity, 80°C: about 60% of maximal activity Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 1 h, 5% loss of activity Thermus thermophilus
80
-
 1 h, 80% loss of activity Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
50
-
 nicotinamide pH and temperature not specified in the publication Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Thermus thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 above 50% of the maximum activity is confirmed at a broad range of pHs from 6.0 to 9.5 Thermus thermophilus

General Information

General Information Comment Organism
malfunction deletion of this gene abolishes nicotinamide deamination activity in crude extracts of Thermus thermophilus and disrupts the NAD+ salvage pathway in Thermus thermophilus. Disruption of the salvage pathway leads to the severe growth retardation at a higher temperature (80°C), owing to the drastic decrease in the intracellular concentrations of NAD+ and NADH Thermus thermophilus
physiological function key enzyme of the NAD+ salvage pathway Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3000
-
 nicotinamide pH and temperature not specified in the publication Thermus thermophilus