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Literature summary for 3.5.1.19 extracted from

  • Wang, X.; Liu, B.; Dou, Y.; Fan, H.; Wang, S.; Li, T.; Ding, C.; Yu, S.
    The Riemerella anatipestifer AS87_01735 gene encodes nicotinamidase PncA, an important virulence factor (2016), Appl. Environ. Microbiol., 82, 5815-5823 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.19

Application

Application Comment Organism
medicine Riemerella anatipestifer AS87_01735 gene encodes PncA, which is an important virulence factor. The Yb2DELTApncA mutant can be used as a novel live vaccine candidate Riemerella anatipestifer

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Riemerella anatipestifer

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Riemerella anatipestifer 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 SDS-PAGE Riemerella anatipestifer

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotinamide + H2O Riemerella anatipestifer nicotinamidase is a key enzyme in the NAD+ salvage pathway nicotinate + NH3
-
 ?
nicotinamide + H2O Riemerella anatipestifer Yb2 nicotinamidase is a key enzyme in the NAD+ salvage pathway nicotinate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Riemerella anatipestifer
-
-
-
Riemerella anatipestifer Yb2
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotinamide + H2O
-
 Riemerella anatipestifer nicotinate + NH3
-
 ?
nicotinamide + H2O nicotinamidase is a key enzyme in the NAD+ salvage pathway Riemerella anatipestifer nicotinate + NH3
-
 ?
nicotinamide + H2O
-
 Riemerella anatipestifer Yb2 nicotinate + NH3
-
 ?
nicotinamide + H2O nicotinamidase is a key enzyme in the NAD+ salvage pathway Riemerella anatipestifer Yb2 nicotinate + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Riemerella anatipestifer

Synonyms

Synonyms Comment Organism
AS87_01735
-
 Riemerella anatipestifer
nicotinamidase PncA
-
 Riemerella anatipestifer

General Information

General Information Comment Organism
malfunction the mutant Yb2DELTApncA shows a similar growth rate but decreased NAD+ quantities in both the exponential and stationary phases in tryptic soy broth culture, compared with the wild-type strain Yb2. In addition, Yb2DELTApncA-infected ducks show much lower bacterial loads in their blood, and no visible histological changes were observed in the heart, liver, and spleen Riemerella anatipestifer
physiological function nicotinamidase is a key enzyme in the NAD+ salvage pathway Riemerella anatipestifer