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Literature summary for 3.5.1.19 extracted from

  • Stekhanova, T.N.; Bezsudnova, E.Y.; Mardanov, A.V.; Osipov, E.M.; Ravin, N.V.; Skryabin, K.G.; Popov, V.O.
    Nicotinamidase from the thermophilic archaeon Acidilobus saccharovorans: structural and functional characteristics (2014), Biochemistry (Moscow), 79, 54-61.
    View publication on PubMed
Search for more literature for 3.5.1.19

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 2 mM, 2fold activation Acidilobus saccharovorans
dithiothreitol 2 mM, 2.1fold activation Acidilobus saccharovorans
EDTA 57% inhibitioin at 10 mM. 10% inhibition at 1 mM Acidilobus saccharovorans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Acidilobus saccharovorans

Inhibitors

Inhibitors Comment Organism Structure
Fe3+ 1 mM, 94% inhibition Acidilobus saccharovorans
NaCl 50 mM, 12% inhibition Acidilobus saccharovorans
Zn2+ 5 mM, complete inhibition Acidilobus saccharovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 nicotinamide pH 7.5, 60°C Acidilobus saccharovorans
0.41
-
 Pyrazinamide pH 7.5, 60°C Acidilobus saccharovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme contains Fe2+ (0.77 mol per mol of protein). 1.6fold activation by 5 mM Fe2+ Acidilobus saccharovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46400
-
-
 Acidilobus saccharovorans

Organism

Organism UniProt Comment Textmining
Acidilobus saccharovorans D9Q1R5
-
-
Acidilobus saccharovorans DSM 16705 D9Q1R5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with N-carbamoylsarcosine, hydantoic acid, acrylamide, D- and L-asparagine, D- and L-glutamine Acidilobus saccharovorans ?
-
 ?
additional information no activity with N-carbamoylsarcosine, hydantoic acid, acrylamide, D- and L-asparagine, D- and L-glutamine Acidilobus saccharovorans DSM 16705 ?
-
 ?
nicotinamide + H2O
-
 Acidilobus saccharovorans nicotinate + NH3
-
 ?
nicotinamide + H2O
-
 Acidilobus saccharovorans DSM 16705 nicotinate + NH3
-
 ?
pyrazinamide + H2O
-
 Acidilobus saccharovorans pyrazine-2-carboxylic acid + NH3
-
 ?
pyrazinamide + H2O
-
 Acidilobus saccharovorans DSM 16705 pyrazine-2-carboxylic acid + NH3
-
 ?

Subunits

Subunits Comment Organism
homodimer in solution the protein exists as a dimer with a small admixture of tetrameric form Acidilobus saccharovorans

Synonyms

Synonyms Comment Organism
ASAC_0847
-
 Acidilobus saccharovorans
NA_As0847
-
 Acidilobus saccharovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Acidilobus saccharovorans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 100 60°C: about 45% of maximal activity, 100°C: 85% of maximal activity Acidilobus saccharovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 t1/2: 180 min Acidilobus saccharovorans
80
-
 t1/2: 35 min Acidilobus saccharovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
76.9
-
 nicotinamide pH 7.5, 60°C Acidilobus saccharovorans
135.7
-
 Pyrazinamide pH 7.5, 60°C Acidilobus saccharovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Acidilobus saccharovorans

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10 hydrolysis of nicotinamide of a wide range of pH values Acidilobus saccharovorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
331
-
 Pyrazinamide pH 7.5, 60°C Acidilobus saccharovorans
427
-
 nicotinamide pH 7.5, 60°C Acidilobus saccharovorans