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Literature summary for 3.5.1.19 extracted from
- Kinetics and inhibition of nicotinamidase from Mycobacterium tuberculosis (2010), Biochemistry, 49, 9613-9619.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H57D | the mutant has reduced Mn2+ content and shows a 6fold and 38fold decrease in kcat value for nicotinamide and pyrazinamide, respectively | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-pyridine carboxaldehyde | competitive inhibitor | Mycobacterium tuberculosis |  |
| iodoacetamide | reversible, competitive inhibitor | Mycobacterium tuberculosis | |
| pyrazinecarbonitrile | irreversible inactivator | Mycobacterium tuberculosis | |
| pyridine-3-carbonitrile | reversible, competitive inhibitor | Mycobacterium tuberculosis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
nicotinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 0.014 | - |
nicotinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 0.016 | - |
Pyrazinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 0.3 | - |
Pyrazinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | the enzyme contains 0.47 M of Fe2+ per mol of enzyme | Mycobacterium tuberculosis | |
| Mn2+ | the enzyme contains 0.44 M Mn2+ per mol of enzyme | Mycobacterium tuberculosis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinamide + H2O | Mycobacterium tuberculosis | - |
nicotinate + NH3 | - |
? | |
| nicotinamide + H2O | Mycobacterium tuberculosis H37Rv | - |
nicotinate + NH3 | - |
? | |
| pyrazinamide + H2O | Mycobacterium tuberculosis | - |
pyrazine-2-carboxylic acid + NH3 | - |
? | |
| pyrazinamide + H2O | Mycobacterium tuberculosis H37Rv | - |
pyrazine-2-carboxylic acid + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinamide + H2O | - |
Mycobacterium tuberculosis | nicotinate + NH3 | - |
? | |
| nicotinamide + H2O | - |
Mycobacterium tuberculosis H37Rv | nicotinate + NH3 | - |
? | |
| pyrazinamide + H2O | - |
Mycobacterium tuberculosis | pyrazine-2-carboxylic acid + NH3 | - |
? | |
| pyrazinamide + H2O | - |
Mycobacterium tuberculosis H37Rv | pyrazine-2-carboxylic acid + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nicotinamidase/pyrazinamidase | - |
Mycobacterium tuberculosis |
| PncA | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
Pyrazinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 0.5 | - |
nicotinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 3.1 | - |
nicotinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 3.8 | - |
Pyrazinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
around pH 8.0 | Mycobacterium tuberculosis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00029 | - |
3-pyridine carboxaldehyde | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 0.1 | - |
pyridine-3-carbonitrile | Ki about 0.1 mM, wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is involved in the NAD+ salvage pathway | Mycobacterium tuberculosis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.2 | - |
Pyrazinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 13 | - |
Pyrazinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 37 | - |
nicotinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 220 | - |
nicotinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
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