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show all sequences of 3.5.1.19

Characterization of nicotinamidases: steady state kinetic parameters, classwide inhibition by nicotinaldehydes, and catalytic mechanism

French, J.B.; Cen, Y.; Vrablik, T.L.; Xu, P.; Allen, E.; Hanna-Rose, W.; Sauve, A.A.; Biochemistry 49, 10421-10439 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Borreliella burgdorferi
-
Caenorhabditis elegans
-
Plasmodium falciparum
-
Saccharomyces cerevisiae
-
Streptococcus pneumoniae
Engineering
Amino acid exchange
Commentary
Organism
C136A
the mutant is unable to catalyze nicotinamide hydrolysis
Streptococcus pneumoniae
C136S
the mutant is unable to catalyze nicotinamide hydrolysis
Streptococcus pneumoniae
K103A
the mutant has only 0.15% of the catalytic activity of the native enzyme
Streptococcus pneumoniae
R97A
the mutant is a robust nicotinamidase and is nearly as good as the native enzyme
Streptococcus pneumoniae
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-chloropyridine-3-carbaldehyde
-
Borreliella burgdorferi
2-chloropyridine-3-carbaldehyde
-
Saccharomyces cerevisiae
3-acetylpyridine
-
Borreliella burgdorferi
3-acetylpyridine
-
Plasmodium falciparum
3-acetylpyridine
-
Saccharomyces cerevisiae
3-acetylpyridine
-
Streptococcus pneumoniae
3-Cyanopyridine
-
Borreliella burgdorferi
3-Cyanopyridine
-
Plasmodium falciparum
3-Cyanopyridine
-
Saccharomyces cerevisiae
3-Cyanopyridine
-
Streptococcus pneumoniae
3-hydroxypyridine
-
Streptococcus pneumoniae
4-methoxylnicotinaldehyde
weak inhibitor
Borreliella burgdorferi
4-methoxylnicotinaldehyde
weak inhibitor
Caenorhabditis elegans
4-methoxylnicotinaldehyde
weak inhibitor
Plasmodium falciparum
4-methoxylnicotinaldehyde
weak inhibitor
Saccharomyces cerevisiae
4-methoxylnicotinaldehyde
weak inhibitor
Streptococcus pneumoniae
5-bromopyridine-3-carbaldehyde
potent inhibitor
Plasmodium falciparum
5-bromopyridine-3-carbaldehyde
potent inhibitor
Saccharomyces cerevisiae
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Borreliella burgdorferi
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Caenorhabditis elegans
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Plasmodium falciparum
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Saccharomyces cerevisiae
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Streptococcus pneumoniae
5-methylnicotinaldehyde
-
Borreliella burgdorferi
5-methylnicotinaldehyde
-
Caenorhabditis elegans
5-methylnicotinaldehyde
-
Plasmodium falciparum
5-methylnicotinaldehyde
-
Saccharomyces cerevisiae
5-methylnicotinaldehyde
-
Streptococcus pneumoniae
5-methylpyridine-3-carbaldehyde
potent inhibitor
Borreliella burgdorferi
5-methylpyridine-3-carbaldehyde
potent inhibitor
Plasmodium falciparum
5-methylpyridine-3-carbaldehyde
potent inhibitor
Saccharomyces cerevisiae
6-fluoropyridine-3-carbaldehyde
-
Borreliella burgdorferi
6-fluoropyridine-3-carbaldehyde
-
Plasmodium falciparum
additional information
not inhibited by 3-hydroxypyridine
Borreliella burgdorferi
additional information
not inhibited by nicotinic acid
Caenorhabditis elegans
additional information
not inhibited by 3-hydroxypyridine and 2-chloropyridine-3-carbaldehyde
Plasmodium falciparum
additional information
not inhibited by 3-hydroxypyridine and 6-fluoropyridine-3-carbaldehyde
Saccharomyces cerevisiae
nicotinaldehyde
potent competitive inhibitor
Borreliella burgdorferi
nicotinaldehyde
potent competitive inhibitor
Caenorhabditis elegans
nicotinaldehyde
potent competitive inhibitor
Plasmodium falciparum
nicotinaldehyde
potent competitive inhibitor
Saccharomyces cerevisiae
nicotinaldehyde
potent competitive inhibitor
Streptococcus pneumoniae
nicotinic acid
-
Borreliella burgdorferi
nicotinic acid
-
Plasmodium falciparum
nicotinic acid
-
Saccharomyces cerevisiae
nicotinic acid
-
Streptococcus pneumoniae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0027
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
0.003
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
0.0106
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
0.0367
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
0.076
-
nicotinamide
wild type enzyme isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
0.113
-
nicotinamide
wild type enzyme isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-methoxypyridine-3-carboxamide + H2O
Plasmodium falciparum
12% activity compared to nicotinamide
?
-
-
?
4-methoxypyridine-3-carboxamide + H2O
Borreliella burgdorferi
20% activity compared to nicotinamide
?
-
-
?
4-methoxypyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
5% activity compared to nicotinamide
?
-
-
?
4-methoxypyridine-3-carboxamide + H2O
Streptococcus pneumoniae
6.5% activity compared to nicotinamide
?
-
-
?
5-methoxypyridine-3-carboxamide + H2O
Streptococcus pneumoniae
137% activity compared to nicotinamide
?
-
-
?
5-methoxypyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
206% activity compared to nicotinamide
?
-
-
?
5-methoxypyridine-3-carboxamide + H2O
Borreliella burgdorferi
221% activity compared to nicotinamide
?
-
-
?
5-methoxypyridine-3-carboxamide + H2O
Plasmodium falciparum
646% activity compared to nicotinamide
?
-
-
?
5-methylpyridine-3-carboxamide + H2O
Streptococcus pneumoniae
163% activity compared to nicotinamide
?
-
-
?
5-methylpyridine-3-carboxamide + H2O
Plasmodium falciparum
330% activity compared to nicotinamide
?
-
-
?
5-methylpyridine-3-carboxamide + H2O
Borreliella burgdorferi
51% activity compared to nicotinamide
?
-
-
?
5-methylpyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
80% activity compared to nicotinamide
?
-
-
?
6-aminopyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
20% activity compared to nicotinamide
?
-
-
?
6-aminopyridine-3-carboxamide + H2O
Streptococcus pneumoniae
36% activity compared to nicotinamide
?
-
-
?
6-aminopyridine-3-carboxamide + H2O
Plasmodium falciparum
45% activity compared to nicotinamide
?
-
-
?
6-chloropyridine-3-carboxamide + H2O
Streptococcus pneumoniae
1.8% activity compared to nicotinamide
?
-
-
?
6-chloropyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
17% activity compared to nicotinamide
?
-
-
?
6-methylpyridine-3-carboxamide + H2O
Streptococcus pneumoniae
11% activity compared to nicotinamide
?
-
-
?
6-methylpyridine-3-carboxamide + H2O
Plasmodium falciparum
40% activity compared to nicotinamide
?
-
-
?
6-methylpyridine-3-carboxamide + H2O
Borreliella burgdorferi
45% activity compared to nicotinamide
?
-
-
?
ethyl pyridine-3-carboxylate + H2O
Streptococcus pneumoniae
0.86% activity compared to nicotinamide
?
-
-
?
ethyl pyridine-3-carboxylate + H2O
Plasmodium falciparum
2.3% activity compared to nicotinamide
?
-
-
?
ethyl pyridine-3-carboxylate + H2O
Saccharomyces cerevisiae
4.4% activity compared to nicotinamide
?
-
-
?
methyl pyridine-3-carboxylate + H2O
Saccharomyces cerevisiae
15% activity compared to nicotinamide
?
-
-
?
methyl pyridine-3-carboxylate + H2O
Streptococcus pneumoniae
3.5% activity compared to nicotinamide
?
-
-
?
methyl pyridine-3-carboxylate + H2O
Plasmodium falciparum
6500% activity compared to nicotinamide
?
-
-
?
additional information
Saccharomyces cerevisiae
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
?
-
-
-
additional information
Plasmodium falciparum
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
?
-
-
-
additional information
Borreliella burgdorferi
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
?
-
-
-
additional information
Streptococcus pneumoniae
nicotinamidase cannot hydrolyze 1-methylisonicotinamide
?
-
-
-
additional information
Caenorhabditis elegans
nicotinamidase cannot hydrolyze 1-methylisonicotinamide
?
-
-
-
nicotinamide + H2O
Saccharomyces cerevisiae
100% activity
nicotinate + NH3
-
-
?
nicotinamide + H2O
Streptococcus pneumoniae
100% activity
nicotinate + NH3
-
-
?
nicotinamide + H2O
Caenorhabditis elegans
100% activity
nicotinate + NH3
-
-
?
nicotinamide + H2O
Plasmodium falciparum
100% activity
nicotinate + NH3
-
-
?
nicotinamide + H2O
Borreliella burgdorferi
100% activity
nicotinate + NH3
-
-
?
phenyl pyridine-3-carboxylate + H2O
Plasmodium falciparum
13% activity compared to nicotinamide
?
-
-
?
phenyl pyridine-3-carboxylate + H2O
Saccharomyces cerevisiae
3.1% activity compared to nicotinamide
?
-
-
?
phenyl pyridine-3-carboxylate + H2O
Streptococcus pneumoniae
3.1% activity compared to nicotinamide
?
-
-
?
pyrazinamide + H2O
Saccharomyces cerevisiae
-
pyrazine-2-carboxylic acid + NH3
-
-
?
pyrazinamide + H2O
Streptococcus pneumoniae
-
pyrazine-2-carboxylic acid + NH3
-
-
?
pyrazinamide + H2O
Caenorhabditis elegans
-
pyrazine-2-carboxylic acid + NH3
-
-
?
pyrazinamide + H2O
Plasmodium falciparum
-
pyrazine-2-carboxylic acid + NH3
-
-
?
pyrazinamide + H2O
Borreliella burgdorferi
-
pyrazine-2-carboxylic acid + NH3
-
-
?
pyridine-3-carbothioamide + H2O
Saccharomyces cerevisiae
44% activity compared to nicotinamide
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Borreliella burgdorferi
-
-
-
Caenorhabditis elegans
-
-
-
Plasmodium falciparum
-
-
-
Saccharomyces cerevisiae
-
-
-
Streptococcus pneumoniae
-
-
-
Purification (Commentary)
Commentary
Organism
-
Borreliella burgdorferi
-
Caenorhabditis elegans
-
Plasmodium falciparum
-
Saccharomyces cerevisiae
-
Streptococcus pneumoniae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-methoxypyridine-3-carboxamide + H2O
12% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
4-methoxypyridine-3-carboxamide + H2O
20% activity compared to nicotinamide
718859
Borreliella burgdorferi
?
-
-
-
?
4-methoxypyridine-3-carboxamide + H2O
5% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
4-methoxypyridine-3-carboxamide + H2O
6.5% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
5-methoxypyridine-3-carboxamide + H2O
137% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
5-methoxypyridine-3-carboxamide + H2O
206% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
5-methoxypyridine-3-carboxamide + H2O
221% activity compared to nicotinamide
718859
Borreliella burgdorferi
?
-
-
-
?
5-methoxypyridine-3-carboxamide + H2O
646% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
5-methylpyridine-3-carboxamide + H2O
163% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
5-methylpyridine-3-carboxamide + H2O
330% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
5-methylpyridine-3-carboxamide + H2O
51% activity compared to nicotinamide
718859
Borreliella burgdorferi
?
-
-
-
?
5-methylpyridine-3-carboxamide + H2O
80% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
6-aminopyridine-3-carboxamide + H2O
20% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
6-aminopyridine-3-carboxamide + H2O
36% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
6-aminopyridine-3-carboxamide + H2O
45% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
6-chloropyridine-3-carboxamide + H2O
1.8% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
6-chloropyridine-3-carboxamide + H2O
17% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
6-methylpyridine-3-carboxamide + H2O
11% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
6-methylpyridine-3-carboxamide + H2O
40% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
6-methylpyridine-3-carboxamide + H2O
45% activity compared to nicotinamide
718859
Borreliella burgdorferi
?
-
-
-
?
ethyl pyridine-3-carboxylate + H2O
0.86% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
ethyl pyridine-3-carboxylate + H2O
2.3% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
ethyl pyridine-3-carboxylate + H2O
4.4% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
methyl pyridine-3-carboxylate + H2O
15% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
methyl pyridine-3-carboxylate + H2O
3.5% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
methyl pyridine-3-carboxylate + H2O
6500% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
additional information
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
-
additional information
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
718859
Plasmodium falciparum
?
-
-
-
-
additional information
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
718859
Borreliella burgdorferi
?
-
-
-
-
additional information
nicotinamidase cannot hydrolyze 1-methylisonicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
-
additional information
nicotinamidase cannot hydrolyze 1-methylisonicotinamide
718859
Caenorhabditis elegans
?
-
-
-
-
nicotinamide + H2O
100% activity
718859
Saccharomyces cerevisiae
nicotinate + NH3
-
-
-
?
nicotinamide + H2O
100% activity
718859
Streptococcus pneumoniae
nicotinate + NH3
-
-
-
?
nicotinamide + H2O
100% activity
718859
Caenorhabditis elegans
nicotinate + NH3
-
-
-
?
nicotinamide + H2O
100% activity
718859
Plasmodium falciparum
nicotinate + NH3
-
-
-
?
nicotinamide + H2O
100% activity
718859
Borreliella burgdorferi
nicotinate + NH3
-
-
-
?
phenyl pyridine-3-carboxylate + H2O
13% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
phenyl pyridine-3-carboxylate + H2O
3.1% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
phenyl pyridine-3-carboxylate + H2O
3.1% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
pyrazinamide + H2O
-
718859
Saccharomyces cerevisiae
pyrazine-2-carboxylic acid + NH3
-
-
-
?
pyrazinamide + H2O
-
718859
Streptococcus pneumoniae
pyrazine-2-carboxylic acid + NH3
-
-
-
?
pyrazinamide + H2O
-
718859
Caenorhabditis elegans
pyrazine-2-carboxylic acid + NH3
-
-
-
?
pyrazinamide + H2O
-
718859
Plasmodium falciparum
pyrazine-2-carboxylic acid + NH3
-
-
-
?
pyrazinamide + H2O
-
718859
Borreliella burgdorferi
pyrazine-2-carboxylic acid + NH3
-
-
-
?
pyridine-3-carbothioamide + H2O
44% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.003
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
0.28
-
nicotinamide
wild type enzyme isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
0.3
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
2.1
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
3.1
-
nicotinamide
wild type enzyme isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.8
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.000011
-
nicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
0.000022
-
nicotinaldehyde
isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
0.000023
-
5-methylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
0.000023
-
5-methylpyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
0.000034
-
nicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
0.000039
-
5-methoxypyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
0.000056
-
5-methoxypyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
0.00011
-
nicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
0.00011
-
nicotinaldehyde
isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
0.00014
-
5-methoxypyridine-3-carbaldehyde
isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
0.00019
-
5-methylpyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
0.00031
-
5-methoxypyridine-3-carbaldehyde
isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
0.00044
-
4-methoxylnicotinaldehyde
isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
0.00059
-
5-bromopyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
0.00065
-
5-methylpyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
0.00085
-
5-methoxypyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
0.001
-
4-methoxylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
0.0014
-
nicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
0.0038
-
5-methoxypyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
0.004
-
5-bromopyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
0.004
-
6-fluoropyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
0.018
-
6-fluoropyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
0.05
-
4-methoxylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
0.068
-
4-methoxylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
0.085
-
3-Cyanopyridine
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
0.153
-
4-methoxylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
0.37
-
2-chloropyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
0.5
-
3-Cyanopyridine
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
1
-
3-Cyanopyridine
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
2
-
3-Cyanopyridine
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
2
-
nicotinic acid
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
5
-
2-chloropyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Borreliella burgdorferi
-
Caenorhabditis elegans
-
Plasmodium falciparum
-
Saccharomyces cerevisiae
-
Streptococcus pneumoniae
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C136A
the mutant is unable to catalyze nicotinamide hydrolysis
Streptococcus pneumoniae
C136S
the mutant is unable to catalyze nicotinamide hydrolysis
Streptococcus pneumoniae
K103A
the mutant has only 0.15% of the catalytic activity of the native enzyme
Streptococcus pneumoniae
R97A
the mutant is a robust nicotinamidase and is nearly as good as the native enzyme
Streptococcus pneumoniae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-chloropyridine-3-carbaldehyde
-
Borreliella burgdorferi
2-chloropyridine-3-carbaldehyde
-
Saccharomyces cerevisiae
3-acetylpyridine
-
Borreliella burgdorferi
3-acetylpyridine
-
Plasmodium falciparum
3-acetylpyridine
-
Saccharomyces cerevisiae
3-acetylpyridine
-
Streptococcus pneumoniae
3-Cyanopyridine
-
Borreliella burgdorferi
3-Cyanopyridine
-
Plasmodium falciparum
3-Cyanopyridine
-
Saccharomyces cerevisiae
3-Cyanopyridine
-
Streptococcus pneumoniae
3-hydroxypyridine
-
Streptococcus pneumoniae
4-methoxylnicotinaldehyde
weak inhibitor
Borreliella burgdorferi
4-methoxylnicotinaldehyde
weak inhibitor
Caenorhabditis elegans
4-methoxylnicotinaldehyde
weak inhibitor
Plasmodium falciparum
4-methoxylnicotinaldehyde
weak inhibitor
Saccharomyces cerevisiae
4-methoxylnicotinaldehyde
weak inhibitor
Streptococcus pneumoniae
5-bromopyridine-3-carbaldehyde
potent inhibitor
Plasmodium falciparum
5-bromopyridine-3-carbaldehyde
potent inhibitor
Saccharomyces cerevisiae
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Borreliella burgdorferi
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Caenorhabditis elegans
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Plasmodium falciparum
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Saccharomyces cerevisiae
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Streptococcus pneumoniae
5-methylnicotinaldehyde
-
Borreliella burgdorferi
5-methylnicotinaldehyde
-
Caenorhabditis elegans
5-methylnicotinaldehyde
-
Plasmodium falciparum
5-methylnicotinaldehyde
-
Saccharomyces cerevisiae
5-methylnicotinaldehyde
-
Streptococcus pneumoniae
5-methylpyridine-3-carbaldehyde
potent inhibitor
Borreliella burgdorferi
5-methylpyridine-3-carbaldehyde
potent inhibitor
Plasmodium falciparum
5-methylpyridine-3-carbaldehyde
potent inhibitor
Saccharomyces cerevisiae
6-fluoropyridine-3-carbaldehyde
-
Borreliella burgdorferi
6-fluoropyridine-3-carbaldehyde
-
Plasmodium falciparum
additional information
not inhibited by 3-hydroxypyridine
Borreliella burgdorferi
additional information
not inhibited by nicotinic acid
Caenorhabditis elegans
additional information
not inhibited by 3-hydroxypyridine and 2-chloropyridine-3-carbaldehyde
Plasmodium falciparum
additional information
not inhibited by 3-hydroxypyridine and 6-fluoropyridine-3-carbaldehyde
Saccharomyces cerevisiae
nicotinaldehyde
potent competitive inhibitor
Borreliella burgdorferi
nicotinaldehyde
potent competitive inhibitor
Caenorhabditis elegans
nicotinaldehyde
potent competitive inhibitor
Plasmodium falciparum
nicotinaldehyde
potent competitive inhibitor
Saccharomyces cerevisiae
nicotinaldehyde
potent competitive inhibitor
Streptococcus pneumoniae
nicotinic acid
-
Borreliella burgdorferi
nicotinic acid
-
Plasmodium falciparum
nicotinic acid
-
Saccharomyces cerevisiae
nicotinic acid
-
Streptococcus pneumoniae
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.000011
-
nicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
0.000022
-
nicotinaldehyde
isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
0.000023
-
5-methylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
0.000023
-
5-methylpyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
0.000034
-
nicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
0.000039
-
5-methoxypyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
0.000056
-
5-methoxypyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
0.00011
-
nicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
0.00011
-
nicotinaldehyde
isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
0.00014
-
5-methoxypyridine-3-carbaldehyde
isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
0.00019
-
5-methylpyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
0.00031
-
5-methoxypyridine-3-carbaldehyde
isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
0.00044
-
4-methoxylnicotinaldehyde
isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
0.00059
-
5-bromopyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
0.00065
-
5-methylpyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
0.00085
-
5-methoxypyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
0.001
-
4-methoxylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
0.0014
-
nicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
0.0038
-
5-methoxypyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
0.004
-
5-bromopyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
0.004
-
6-fluoropyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
0.018
-
6-fluoropyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
0.05
-
4-methoxylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
0.068
-
4-methoxylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
0.085
-
3-Cyanopyridine
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
0.153
-
4-methoxylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
0.37
-
2-chloropyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
0.5
-
3-Cyanopyridine
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
1
-
3-Cyanopyridine
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
2
-
3-Cyanopyridine
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
2
-
nicotinic acid
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
5
-
2-chloropyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0027
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
0.003
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
0.0106
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
0.0367
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
0.076
-
nicotinamide
wild type enzyme isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
0.113
-
nicotinamide
wild type enzyme isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-methoxypyridine-3-carboxamide + H2O
Plasmodium falciparum
12% activity compared to nicotinamide
?
-
-
?
4-methoxypyridine-3-carboxamide + H2O
Borreliella burgdorferi
20% activity compared to nicotinamide
?
-
-
?
4-methoxypyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
5% activity compared to nicotinamide
?
-
-
?
4-methoxypyridine-3-carboxamide + H2O
Streptococcus pneumoniae
6.5% activity compared to nicotinamide
?
-
-
?
5-methoxypyridine-3-carboxamide + H2O
Streptococcus pneumoniae
137% activity compared to nicotinamide
?
-
-
?
5-methoxypyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
206% activity compared to nicotinamide
?
-
-
?
5-methoxypyridine-3-carboxamide + H2O
Borreliella burgdorferi
221% activity compared to nicotinamide
?
-
-
?
5-methoxypyridine-3-carboxamide + H2O
Plasmodium falciparum
646% activity compared to nicotinamide
?
-
-
?
5-methylpyridine-3-carboxamide + H2O
Streptococcus pneumoniae
163% activity compared to nicotinamide
?
-
-
?
5-methylpyridine-3-carboxamide + H2O
Plasmodium falciparum
330% activity compared to nicotinamide
?
-
-
?
5-methylpyridine-3-carboxamide + H2O
Borreliella burgdorferi
51% activity compared to nicotinamide
?
-
-
?
5-methylpyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
80% activity compared to nicotinamide
?
-
-
?
6-aminopyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
20% activity compared to nicotinamide
?
-
-
?
6-aminopyridine-3-carboxamide + H2O
Streptococcus pneumoniae
36% activity compared to nicotinamide
?
-
-
?
6-aminopyridine-3-carboxamide + H2O
Plasmodium falciparum
45% activity compared to nicotinamide
?
-
-
?
6-chloropyridine-3-carboxamide + H2O
Streptococcus pneumoniae
1.8% activity compared to nicotinamide
?
-
-
?
6-chloropyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
17% activity compared to nicotinamide
?
-
-
?
6-methylpyridine-3-carboxamide + H2O
Streptococcus pneumoniae
11% activity compared to nicotinamide
?
-
-
?
6-methylpyridine-3-carboxamide + H2O
Plasmodium falciparum
40% activity compared to nicotinamide
?
-
-
?
6-methylpyridine-3-carboxamide + H2O
Borreliella burgdorferi
45% activity compared to nicotinamide
?
-
-
?
ethyl pyridine-3-carboxylate + H2O
Streptococcus pneumoniae
0.86% activity compared to nicotinamide
?
-
-
?
ethyl pyridine-3-carboxylate + H2O
Plasmodium falciparum
2.3% activity compared to nicotinamide
?
-
-
?
ethyl pyridine-3-carboxylate + H2O
Saccharomyces cerevisiae
4.4% activity compared to nicotinamide
?
-
-
?
methyl pyridine-3-carboxylate + H2O
Saccharomyces cerevisiae
15% activity compared to nicotinamide
?
-
-
?
methyl pyridine-3-carboxylate + H2O
Streptococcus pneumoniae
3.5% activity compared to nicotinamide
?
-
-
?
methyl pyridine-3-carboxylate + H2O
Plasmodium falciparum
6500% activity compared to nicotinamide
?
-
-
?
additional information
Saccharomyces cerevisiae
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
?
-
-
-
additional information
Plasmodium falciparum
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
?
-
-
-
additional information
Borreliella burgdorferi
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
?
-
-
-
additional information
Streptococcus pneumoniae
nicotinamidase cannot hydrolyze 1-methylisonicotinamide
?
-
-
-
additional information
Caenorhabditis elegans
nicotinamidase cannot hydrolyze 1-methylisonicotinamide
?
-
-
-
nicotinamide + H2O
Saccharomyces cerevisiae
100% activity
nicotinate + NH3
-
-
?
nicotinamide + H2O
Streptococcus pneumoniae
100% activity
nicotinate + NH3
-
-
?
nicotinamide + H2O
Caenorhabditis elegans
100% activity
nicotinate + NH3
-
-
?
nicotinamide + H2O
Plasmodium falciparum
100% activity
nicotinate + NH3
-
-
?
nicotinamide + H2O
Borreliella burgdorferi
100% activity
nicotinate + NH3
-
-
?
phenyl pyridine-3-carboxylate + H2O
Plasmodium falciparum
13% activity compared to nicotinamide
?
-
-
?
phenyl pyridine-3-carboxylate + H2O
Saccharomyces cerevisiae
3.1% activity compared to nicotinamide
?
-
-
?
phenyl pyridine-3-carboxylate + H2O
Streptococcus pneumoniae
3.1% activity compared to nicotinamide
?
-
-
?
pyrazinamide + H2O
Saccharomyces cerevisiae
-
pyrazine-2-carboxylic acid + NH3
-
-
?
pyrazinamide + H2O
Streptococcus pneumoniae
-
pyrazine-2-carboxylic acid + NH3
-
-
?
pyrazinamide + H2O
Caenorhabditis elegans
-
pyrazine-2-carboxylic acid + NH3
-
-
?
pyrazinamide + H2O
Plasmodium falciparum
-
pyrazine-2-carboxylic acid + NH3
-
-
?
pyrazinamide + H2O
Borreliella burgdorferi
-
pyrazine-2-carboxylic acid + NH3
-
-
?
pyridine-3-carbothioamide + H2O
Saccharomyces cerevisiae
44% activity compared to nicotinamide
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Borreliella burgdorferi
-
Caenorhabditis elegans
-
Plasmodium falciparum
-
Saccharomyces cerevisiae
-
Streptococcus pneumoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-methoxypyridine-3-carboxamide + H2O
12% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
4-methoxypyridine-3-carboxamide + H2O
20% activity compared to nicotinamide
718859
Borreliella burgdorferi
?
-
-
-
?
4-methoxypyridine-3-carboxamide + H2O
5% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
4-methoxypyridine-3-carboxamide + H2O
6.5% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
5-methoxypyridine-3-carboxamide + H2O
137% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
5-methoxypyridine-3-carboxamide + H2O
206% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
5-methoxypyridine-3-carboxamide + H2O
221% activity compared to nicotinamide
718859
Borreliella burgdorferi
?
-
-
-
?
5-methoxypyridine-3-carboxamide + H2O
646% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
5-methylpyridine-3-carboxamide + H2O
163% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
5-methylpyridine-3-carboxamide + H2O
330% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
5-methylpyridine-3-carboxamide + H2O
51% activity compared to nicotinamide
718859
Borreliella burgdorferi
?
-
-
-
?
5-methylpyridine-3-carboxamide + H2O
80% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
6-aminopyridine-3-carboxamide + H2O
20% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
6-aminopyridine-3-carboxamide + H2O
36% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
6-aminopyridine-3-carboxamide + H2O
45% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
6-chloropyridine-3-carboxamide + H2O
1.8% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
6-chloropyridine-3-carboxamide + H2O
17% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
6-methylpyridine-3-carboxamide + H2O
11% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
6-methylpyridine-3-carboxamide + H2O
40% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
6-methylpyridine-3-carboxamide + H2O
45% activity compared to nicotinamide
718859
Borreliella burgdorferi
?
-
-
-
?
ethyl pyridine-3-carboxylate + H2O
0.86% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
ethyl pyridine-3-carboxylate + H2O
2.3% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
ethyl pyridine-3-carboxylate + H2O
4.4% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
methyl pyridine-3-carboxylate + H2O
15% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
methyl pyridine-3-carboxylate + H2O
3.5% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
methyl pyridine-3-carboxylate + H2O
6500% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
additional information
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
-
additional information
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
718859
Plasmodium falciparum
?
-
-
-
-
additional information
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
718859
Borreliella burgdorferi
?
-
-
-
-
additional information
nicotinamidase cannot hydrolyze 1-methylisonicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
-
additional information
nicotinamidase cannot hydrolyze 1-methylisonicotinamide
718859
Caenorhabditis elegans
?
-
-
-
-
nicotinamide + H2O
100% activity
718859
Saccharomyces cerevisiae
nicotinate + NH3
-
-
-
?
nicotinamide + H2O
100% activity
718859
Streptococcus pneumoniae
nicotinate + NH3
-
-
-
?
nicotinamide + H2O
100% activity
718859
Caenorhabditis elegans
nicotinate + NH3
-
-
-
?
nicotinamide + H2O
100% activity
718859
Plasmodium falciparum
nicotinate + NH3
-
-
-
?
nicotinamide + H2O
100% activity
718859
Borreliella burgdorferi
nicotinate + NH3
-
-
-
?
phenyl pyridine-3-carboxylate + H2O
13% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
phenyl pyridine-3-carboxylate + H2O
3.1% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
phenyl pyridine-3-carboxylate + H2O
3.1% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
pyrazinamide + H2O
-
718859
Saccharomyces cerevisiae
pyrazine-2-carboxylic acid + NH3
-
-
-
?
pyrazinamide + H2O
-
718859
Streptococcus pneumoniae
pyrazine-2-carboxylic acid + NH3
-
-
-
?
pyrazinamide + H2O
-
718859
Caenorhabditis elegans
pyrazine-2-carboxylic acid + NH3
-
-
-
?
pyrazinamide + H2O
-
718859
Plasmodium falciparum
pyrazine-2-carboxylic acid + NH3
-
-
-
?
pyrazinamide + H2O
-
718859
Borreliella burgdorferi
pyrazine-2-carboxylic acid + NH3
-
-
-
?
pyridine-3-carbothioamide + H2O
44% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.003
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
0.28
-
nicotinamide
wild type enzyme isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
0.3
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
2.1
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
3.1
-
nicotinamide
wild type enzyme isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.8
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
General Information
General Information
Commentary
Organism
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Borreliella burgdorferi
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Caenorhabditis elegans
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Plasmodium falciparum
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Saccharomyces cerevisiae
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Streptococcus pneumoniae
General Information (protein specific)
General Information
Commentary
Organism
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Borreliella burgdorferi
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Caenorhabditis elegans
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Plasmodium falciparum
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Saccharomyces cerevisiae
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Streptococcus pneumoniae
Other publictions for EC 3.5.1.19
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727053
Stekhanova
Nicotinamidase from the thermo ...
Acidilobus saccharovorans, Acidilobus saccharovorans DSM 16705
Biochemistry
79
54-61
2014
2
-
1
-
-
-
4
1
-
1
1
-
-
7
-
-
-
-
-
-
-
-
2
1
1
1
2
1
1
-
-
-
-
-
-
2
-
1
-
-
-
-
-
4
-
1
-
1
1
-
-
-
-
-
-
-
-
-
2
1
1
1
2
1
1
-
-
-
-
1
1
-
1
1
729184
Stekhanova
Nicotinamidase from the thermo ...
Acidilobus saccharovorans, Acidilobus saccharovorans DSM 16705
Biochemistry (Moscow)
79
54-61
2014
3
-
1
-
-
-
3
2
-
1
1
-
-
7
-
-
-
-
-
-
-
-
6
1
1
1
2
2
1
1
-
-
-
-
-
3
-
1
-
-
-
-
-
3
-
2
-
1
1
-
-
-
-
-
-
-
-
-
6
1
1
1
2
2
1
1
-
-
-
-
-
-
2
2
734741
Ion
A multi-scale computational st ...
Streptococcus pneumoniae
Molecules
19
15735-15753
2014
-
1
-
-
-
-
-
-
-
1
-
1
-
2
-
-
-
1
-
-
-
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2
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-
-
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-
-
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1
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-
-
-
-
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-
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1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
735324
Rueda
Nicotinamidase/pyrazinamidase ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Tuberculosis
94
644-648
2014
-
-
1
-
-
-
-
-
-
-
1
4
-
3
-
-
1
-
-
-
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
4
-
-
-
1
-
-
-
-
4
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
735084
Sanchez-Carron
Biochemical and mutational ana ...
Oceanobacillus iheyensis, Oceanobacillus iheyensis HTE831
PLoS ONE
8
e56727
2013
-
-
1
-
9
-
2
27
-
2
2
4
-
5
-
-
1
-
-
-
28
-
12
1
1
1
1
26
1
1
1
-
2
-
-
-
-
1
-
-
9
-
-
2
2
27
-
2
2
4
-
-
-
1
-
-
28
-
12
1
1
1
1
26
1
1
1
-
-
2
2
-
26
26
718924
Smith
Structural and kinetic isotope ...
Saccharomyces cerevisiae
Biochemistry
51
243-256
2012
-
-
1
1
10
-
6
8
-
1
-
1
-
6
-
-
1
-
-
-
-
-
7
-
-
-
-
12
1
-
-
-
5
-
-
-
-
1
-
1
10
-
-
6
5
8
-
1
-
1
-
-
-
1
-
-
-
-
7
-
-
-
-
12
1
-
-
-
-
-
-
-
15
15
719619
Gazanion
Leishmania infantum nicotinami ...
Leishmania infantum
Int. J. Parasitol.
42
323-327
2012
-
-
-
-
1
-
-
-
-
-
-
1
-
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
735054
Zhang
Molecular dynamics simulations ...
Acinetobacter baumannii, Streptococcus pneumoniae
PLoS ONE
7
e39546
2012
-
-
-
2
-
-
-
-
-
-
-
4
-
3
-
-
-
-
-
-
-
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6
-
-
-
-
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-
-
-
-
-
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
720499
Gazanion
The Leishmania nicotinamidase ...
Leishmania infantum, Leishmania infantum MHOM/MA/67/ITMAP-263
Mol. Microbiol.
82
21-38
2011
-
-
1
1
1
-
-
-
1
-
2
4
-
7
-
-
1
-
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
1
-
2
4
-
-
-
1
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
718859
French
Characterization of nicotinami ...
Borreliella burgdorferi, Caenorhabditis elegans, Plasmodium falciparum, Saccharomyces cerevisiae, Streptococcus pneumoniae
Biochemistry
49
10421-10439
2010
-
-
5
-
4
-
46
6
-
-
-
45
-
17
-
-
5
-
-
-
-
-
45
-
-
-
-
6
-
-
-
-
32
-
-
-
-
5
-
-
4
-
-
46
32
6
-
-
-
45
-
-
-
5
-
-
-
-
45
-
-
-
-
6
-
-
-
-
-
5
5
-
-
-
718883
French
High-resolution crystal struct ...
Streptococcus pneumoniae
Biochemistry
49
8803-8812
2010
-
-
1
1
1
-
2
-
-
5
1
1
-
5
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
2
-
-
-
5
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718885
Seiner
Kinetics and inhibition of nic ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Biochemistry
49
9613-9619
2010
-
-
1
-
1
-
4
4
-
2
-
4
-
162
-
-
1
-
-
-
-
-
4
-
-
-
-
4
1
-
-
-
2
-
-
-
-
1
-
-
1
-
-
4
2
4
-
2
-
4
-
-
-
1
-
-
-
-
4
-
-
-
-
4
1
-
-
-
-
1
1
-
4
4
710862
Fyfe
Specificity and mechanism of A ...
Acinetobacter baumannii
Angew. Chem. Int. Ed. Engl.
48
9176-9179
2009
-
-
-
-
-
-
-
-
-
4
1
2
-
2
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
1
2
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
711827
Vrablik
Nicotinamidase modulation of N ...
Caenorhabditis elegans
Development
136
3637-3646
2009
-
-
1
-
1
-
-
-
1
1
-
1
-
4
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
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1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
679806
Zhang
Characterization of Mycobacter ...
Mycobacterium tuberculosis
FEBS J.
275
753-762
2008
-
1
1
-
9
-
-
-
-
2
1
2
-
3
-
-
1
-
-
-
2
-
2
1
1
-
1
-
1
-
1
-
-
-
-
-
1
1
-
-
9
-
-
-
-
-
-
2
1
2
-
-
-
1
-
-
2
-
2
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
697513
Chong
Enhanced tolerance against ear ...
Drosophila melanogaster
Curr. Neurovasc. Res.
5
159-170
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698121
McClure
Pnc1p-mediated nicotinamide cl ...
Saccharomyces cerevisiae
Genetics
180
797-810
2008
-
-
-
-
-
-
-
-
-
-
-
-
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2
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698815
Balan
Life span extension and neuron ...
Drosophila melanogaster
J. Biol. Chem.
283
27810-27819
2008
-
-
1
-
-
-
-
-
-
-
1
-
-
6
-
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1
-
-
1
-
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2
1
-
-
-
-
-
-
-
-
-
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-
-
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1
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-
-
-
-
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1
-
-
-
-
1
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700669
Yin
Changes in pyridine metabolism ...
Lotus japonicus
Phytochemistry
69
2891-2898
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
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-
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1
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677824
Hu
Crystal structure of the yeast ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
461
66-75
2007
-
-
1
1
-
-
-
1
-
1
-
2
-
3
-
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1
-
-
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2
-
2
-
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-
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-
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1
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1
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1
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1
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2
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1
-
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2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681746
van der Horst
The Caenorhabditis elegans nic ...
Caenorhabditis elegans
Mech. Ageing Dev.
128
346-349
2007
-
-
-
-
-
-
-
-
-
-
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1
-
4
-
-
-
-
-
-
-
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1
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1
-
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682376
Wang
Nicotinamidase participates in ...
Arabidopsis thaliana
Plant J.
49
1020-1029
2007
-
-
-
-
-
-
-
1
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-
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1
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6
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-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
-
-
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-
-
-
-
-
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-
-
1
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
682380
Hunt
Nicotinamidase activity is imp ...
Arabidopsis thaliana
Plant J.
51
341-351
2007
2
-
1
-
-
-
3
-
-
-
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1
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3
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1
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-
-
-
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1
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2
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1
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3
-
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1
-
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1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659608
Zheng
Changes in trigonelline (N-met ...
Vigna radiata var. radiata
J. Exp. Bot.
56
1615-1623
2005
-
-
-
-
-
-
-
-
-
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2
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1
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-
-
-
-
3
-
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3
-
1
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-
1
-
-
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-
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-
-
-
-
-
-
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2
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-
3
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
668655
Kim
Brucella abortus nicotinamidas ...
Brucella abortus, Brucella abortus 544 / ATCC 23448
FEMS Microbiol. Lett.
234
289-295
2004
-
-
-
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1
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-
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2
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2
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8
-
-
-
-
-
1
-
-
4
-
-
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-
-
-
-
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-
-
-
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-
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-
1
-
-
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2
-
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2
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-
-
1
-
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4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670237
Gallo
Nicotinamide clearance by Pnc1 ...
Saccharomyces cerevisiae
Mol. Cell. Biol.
24
1301-1312
2004
1
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1
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2
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-
-
-
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1
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2
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1
-
-
-
-
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2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246584
Guo
Mycobacterium smegmatis has tw ...
Mycolicibacterium smegmatis
J. Bacteriol.
182
3881-3884
2000
-
-
1
-
-
-
-
-
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1
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5
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246583
Sun
Reduced pyrazinamidase activit ...
Mycobacterium kansasii
Antimicrob. Agents Chemother.
43
537-542
1999
-
-
-
-
-
-
1
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246585
Boshoff
Purification, gene cloning, ta ...
Mycobacterium tuberculosis, Mycolicibacterium smegmatis
J. Bacteriol.
180
5809-5814
1998
-
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1
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-
-
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2
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5
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1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246587
Raynaud
-
Extracellular enzyme activitie ...
Mycobacterium kansasii, Mycobacterium tuberculosis, Mycobacterium tuberculosis variant bovis, Mycolicibacterium fortuitum, Mycolicibacterium phlei
Microbiology
144
577-587
1998
-
-
-
-
-
-
-
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6
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5
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6
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5
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Frothingham
Identification, cloning, and e ...
Escherichia coli
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40
1426-1431
1996
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1
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2
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246586
Zrez
Increased nicotinamideadenine ...
Plasmodium falciparum
Blood
75
1705-1710
1990
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-
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1
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1
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2
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246588
Kgayama
-
Purification and properties of ...
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Agric. Biol. Chem.
54
2565-2571
1990
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6
1
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2
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1
1
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1
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2
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2
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3
2
2
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1
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1
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6
1
1
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246581
Yan
Purification and characterizat ...
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J. Biol. Chem.
262
9082-9087
1987
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1
1
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2
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1
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2
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1
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2
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1
1
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Shibata
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Tissue distribution of the enz ...
Rattus norvegicus
Agric. Biol. Chem.
50
3037-3041
1986
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3
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3
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1
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209135
Foster
NAD metabolism in Vibrio chole ...
Vibrio cholerae
J. Bacteriol.
149
368-371
1982
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2
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246576
Wintzerith
Purification and characterizat ...
Mus musculus
Biochim. Biophys. Acta
613
191-202
1980
1
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13
2
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3
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1
1
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1
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1
2
1
1
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2
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3
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1
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1
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13
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2
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3
3
1
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1
1
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1
-
1
2
1
1
-
2
-
3
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246577
Fuller
-
Determination of nicotinamide ...
Saccharomyces cerevisiae
Methods Enzymol.
66
3-4
1980
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1
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1
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1
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1
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-
1
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246578
Tanigawa
Nicotinamide deamidase from Fl ...
Flavobacterium peregrinum
Methods Enzymol.
66
132-136
1980
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1
11
1
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1
1
1
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2
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1
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11
-
1
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1
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1
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1
1
1
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-
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-
2
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246575
Foster
Pyridine nucleotide cycle of S ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
138
957-961
1979
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2
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1
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1
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2
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246573
Gadd
-
Kinetic studies of nicotinamid ...
Micrococcus luteus
Int. J. Biochem.
5
397-407
1974
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3
2
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2
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3
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1
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3
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2
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2
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3
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1
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246574
Johnson
-
Inhibition of nicotinamide dea ...
Micrococcus luteus
Int. J. Biochem.
5
633-641
1974
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19
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1
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19
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1
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246572
Gillam
Nicotinamide deamidase from ra ...
Oryctolagus cuniculus
Arch. Biochem. Biophys.
157
268-284
1973
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5
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1
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2
1
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5
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2
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1
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1
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2
1
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246570
Albizati
Active-site studies on rabbit ...
Oryctolagus cuniculus
Biochemistry
11
1508-1516
1972
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7
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1
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1
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2
1
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2
1
1
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7
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1
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1
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2
1
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2
1
1
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246571
Tanigawa
Purification and properties of ...
Flavobacterium peregrinum
J. Biol. Chem.
247
8036-8042
1972
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2
1
1
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1
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1
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6
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2
1
1
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246569
Pardee
Hyperproduction and purificati ...
Escherichia coli
J. Biol. Chem.
246
6792-6796
1971
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1
1
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1
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1
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1
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1
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1
1
1
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2
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1
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246568
Petrack
Nicotinamide deaminase from ma ...
Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
240
1725-1730
1965
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2
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2
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1
1
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1
3
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2
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2
2
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2
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1
1
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246567
Sarma
-
Studies on the enzymes involve ...
Aspergillus niger
Biochim. Biophys. Acta
81
311-322
1964
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4
1
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1
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1
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1
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1
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4
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1
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1
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1
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1
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1
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1
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1
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