Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | activates | Acinetobacter baumannii | |
Mn2+ | activates | Acinetobacter baumannii | |
additional information | PncA is a divalent cation-dependent enzyme | Acinetobacter baumannii | |
Zn2+ | activates, best cation, the active site Cys159 is located at the N-terminus of a6 at one side of the active site with the Zn2+ ion positioned on the other side. The metal ion is bound tightly in the AbPncA active site | Acinetobacter baumannii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
gel filtratrion and analytical ultracentrifugation | Acinetobacter baumannii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinamide + H2O | Acinetobacter baumannii | - |
nicotinate + NH3 | - |
? | |
pyrazinamide + H2O | Acinetobacter baumannii | a nicotinamide prodrug. In Mycobacterium tuberculosis the enzyme converts the nicotinamide analogue prodrug pyrazinamide into the bacteriostatic pyrazinoic acid, hence the alternative name, pyrazinamidase, PncA | pyrazinoic acid + NH3 | a bacteriostatic drug | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baumannii | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
nicotinamide + H2O = nicotinate + NH3 | the active site Cys159, conserved and essential, is located at the N-terminus of a6 at one side of the active site with the Zn2+ ion positioned on the other side. The metal ion is bound tightly in the AbPncA active site. The reaction proceeds in a four-stage mechanism via a tetrahedral and an acyl intermediate, overview | Acinetobacter baumannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinamide + H2O | - |
Acinetobacter baumannii | nicotinate + NH3 | - |
? | |
nicotinamide + H2O | active site structure, overview | Acinetobacter baumannii | nicotinate + NH3 | - |
? | |
pyrazinamide + H2O | a nicotinamide prodrug. In Mycobacterium tuberculosis the enzyme converts the nicotinamide analogue prodrug pyrazinamide into the bacteriostatic pyrazinoic acid, hence the alternative name, pyrazinamidase, PncA | Acinetobacter baumannii | pyrazinoic acid + NH3 | a bacteriostatic drug | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Acinetobacter baumannii |
Synonyms | Comment | Organism |
---|---|---|
PncA | - |
Acinetobacter baumannii |
pyrazinamidase | - |
Acinetobacter baumannii |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the deamination of nicotinamide, which is an important step in the NAD+ salvage pathway. In Mycobacterium tuberculosis the enzyme converts the nicotinamide analogue prodrug pyrazinamide into the bacteriostatic pyrazinoic acid, hence the alternative name, pyrazinamidase, PncA | Acinetobacter baumannii |
physiological function | the reaction product pyrazinoic acid inhibits Mycobacterium tuberculosis type I fatty acid synthase, represses mycolic acid biosynthesis, and appears to affect membrane energetics and acidification of the cytoplasm | Acinetobacter baumannii |