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Literature summary for 3.5.1.19 extracted from
- Characterization of Mycobacterium tuberculosis nicotinamidase/pyrazinamidase (2008), FEBS J., 275, 753-762.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | enzyme is involved in the activation of the antituberculosis drug pyrazinamide | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C138A | significant decrease in enzyme activity | Mycobacterium tuberculosis |
| D49A | significant decrease in enzyme activity | Mycobacterium tuberculosis |
| D8A | significant decrease in enzyme activity | Mycobacterium tuberculosis |
| H51A | significant decrease in enzyme activity | Mycobacterium tuberculosis |
| H57A | significant decrease in enzyme activity | Mycobacterium tuberculosis |
| H71A | significant decrease in enzyme activity | Mycobacterium tuberculosis |
| K96A | significant decrease in enzyme activity | Mycobacterium tuberculosis |
| S104A | partial loss of enzyme activity | Mycobacterium tuberculosis |
| S95A | partial loss of enzyme activity | Mycobacterium tuberculosis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Mycobacterium tuberculosis |  |
| Mn2+ | - |
Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20890 | - |
determined by analytical ultracentrifugation and mass spectrometry | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinamide + H2O | Mycobacterium tuberculosis | - |
NH3 + nicotinic acid | - |
? | |
| pyrazinamide + H2O | Mycobacterium tuberculosis | - |
NH3 + pyrazinoic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel chelate chromatography and molecular sieve | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 81.9 | - |
- |
Mycobacterium tuberculosis |
| 89.6 | - |
- |
Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinamide + H2O | - |
Mycobacterium tuberculosis | NH3 + nicotinic acid | - |
? | |
| pyrazinamide + H2O | - |
Mycobacterium tuberculosis | NH3 + pyrazinoic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nicotinamidase/pyrazinamidase | - |
Mycobacterium tuberculosis |
| PncA | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Mycobacterium tuberculosis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 70 | - |
Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Mycobacterium tuberculosis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Mycobacterium tuberculosis |
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