General Stability | Organism |
---|---|
enzyme is stable in buffer solution containing Co2+ and Ni2+ | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Pseudomonas sp. | |
Hg2+ | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
nicotinamide | - |
Pseudomonas sp. | |
0.056 | - |
Pyrazinamide | - |
Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | enhances activity | Pseudomonas sp. | |
Mn2+ | enhances activity | Pseudomonas sp. | |
Ni2+ | enhances activity | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
2 * 26000, SDS-PAGE | Pseudomonas sp. |
43000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinamide + H2O | Pseudomonas sp. | - |
nicotinate + NH3 | - |
? | |
nicotinamide + H2O | Pseudomonas sp. GDI 211 | - |
nicotinate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. GDI 211 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37.6 | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinamide + H2O | - |
Pseudomonas sp. | nicotinate + NH3 | - |
? | |
nicotinamide + H2O | 59.5% of the activity with pyrazinamide | Pseudomonas sp. | nicotinate + NH3 | - |
? | |
nicotinamide + H2O | - |
Pseudomonas sp. GDI 211 | nicotinate + NH3 | - |
? | |
nicotinamide + H2O | 59.5% of the activity with pyrazinamide | Pseudomonas sp. GDI 211 | nicotinate + NH3 | - |
? | |
pyrazinamide + H2O | - |
Pseudomonas sp. | pyrazinoic acid + NH3 | - |
? | |
pyrazinamide + H2O | - |
Pseudomonas sp. GDI 211 | pyrazinoic acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 26000, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 80 | about 35% of maximal activity at 55°C and at 80°C | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
pH 6.8, in presence of Co2+, 10 min, stable up to | Pseudomonas sp. |
70 | - |
10 min, complete loss of activity | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10.5 | pH 5.0: about 70% of maximal activity, pH 10.5: about 75% of maximal activity | Pseudomonas sp. |