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Literature summary for 3.5.1.18 extracted from

  • Usha, V.; Lloyd, A.J.; Roper, D.I.; Dowson, C.G.; Kozlov, G.; Gehring, K.; Chauhan, S.; Imam, H.T.; Blindauer, C.A.; Besra, G.S.
    Reconstruction of diaminopimelic acid biosynthesis allows characterisation of Mycobacterium tuberculosis N-succinyl-L,L-diaminopimelic acid desuccinylase (2016), Sci. Rep., 6, 23191 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli as an N-terminal hexahistidine ((His)6) tagged fusion containing one zinc ion per DapE monomer Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Mycobacterium tuberculosis
2-thiopheneboronic acid 22.3% inhibition at 10 mM, non-competitive inhibitor Mycobacterium tuberculosis
EDTA
-
Mycobacterium tuberculosis
L-captopril 99.96%, 26.67% and 23.34% inhibition at 10 mM, 3 mM, and 1 mM Mycobacterium tuberculosis
L-Penicillamine 1 mM, 79.41% inhibition Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03109
-
N-succinyl-LL-2,6-diaminoheptanedioate pH 8.0, 37°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ untreated enzyme contains 0.8 Zn2+ per monomer and was enzymatically active Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-succinyl-LL-2,6-diaminoheptanedioate + H2O Mycobacterium tuberculosis
-
succinate + LL-2,6-diaminoheptanedioate
-
?
N-succinyl-LL-2,6-diaminoheptanedioate + H2O Mycobacterium tuberculosis H37Rv
-
succinate + LL-2,6-diaminoheptanedioate
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WHS9
-
-
Mycobacterium tuberculosis H37Rv P9WHS9
-
-

Purification (Commentary)

Purification (Comment) Organism
N-terminal hexahistidine ((His)6) tagged fusion containing one zinc ion per DapE monomer Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-LL-2,6-diaminoheptanedioate + H2O
-
Mycobacterium tuberculosis succinate + LL-2,6-diaminoheptanedioate
-
?
N-succinyl-LL-2,6-diaminoheptanedioate + H2O
-
Mycobacterium tuberculosis H37Rv succinate + LL-2,6-diaminoheptanedioate
-
?

Synonyms

Synonyms Comment Organism
DapE
-
Mycobacterium tuberculosis
Rv1202
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 42 at pH 8.0 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.85
-
N-succinyl-LL-2,6-diaminoheptanedioate pH 8.0, 37°C Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
156
-
N-succinyl-LL-2,6-diaminoheptanedioate pH 8.0, 37°C Mycobacterium tuberculosis