Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli as an N-terminal hexahistidine ((His)6) tagged fusion containing one zinc ion per DapE monomer | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Mycobacterium tuberculosis | |
2-thiopheneboronic acid | 22.3% inhibition at 10 mM, non-competitive inhibitor | Mycobacterium tuberculosis | |
EDTA | - |
Mycobacterium tuberculosis | |
L-captopril | 99.96%, 26.67% and 23.34% inhibition at 10 mM, 3 mM, and 1 mM | Mycobacterium tuberculosis | |
L-Penicillamine | 1 mM, 79.41% inhibition | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03109 | - |
N-succinyl-LL-2,6-diaminoheptanedioate | pH 8.0, 37°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | untreated enzyme contains 0.8 Zn2+ per monomer and was enzymatically active | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl-LL-2,6-diaminoheptanedioate + H2O | Mycobacterium tuberculosis | - |
succinate + LL-2,6-diaminoheptanedioate | - |
? | |
N-succinyl-LL-2,6-diaminoheptanedioate + H2O | Mycobacterium tuberculosis H37Rv | - |
succinate + LL-2,6-diaminoheptanedioate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WHS9 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WHS9 | - |
- |
Purification (Comment) | Organism |
---|---|
N-terminal hexahistidine ((His)6) tagged fusion containing one zinc ion per DapE monomer | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl-LL-2,6-diaminoheptanedioate + H2O | - |
Mycobacterium tuberculosis | succinate + LL-2,6-diaminoheptanedioate | - |
? | |
N-succinyl-LL-2,6-diaminoheptanedioate + H2O | - |
Mycobacterium tuberculosis H37Rv | succinate + LL-2,6-diaminoheptanedioate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DapE | - |
Mycobacterium tuberculosis |
Rv1202 | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 42 | at pH 8.0 | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.85 | - |
N-succinyl-LL-2,6-diaminoheptanedioate | pH 8.0, 37°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
156 | - |
N-succinyl-LL-2,6-diaminoheptanedioate | pH 8.0, 37°C | Mycobacterium tuberculosis |