Cloned (Comment) | Organism |
---|---|
gene dapE, sequence comparison | Haemophilus influenzae |
Crystallization (Comment) | Organism |
---|---|
crystal structure, PDB ID 3IC1, analysis, comparison with the structure of N-acetyl-L-ornithine deacetylase, EC 3.5.1.16, overview | Haemophilus influenzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required, the enzyme binds two Zn(II) ions in non-interactive binding sites with Kd values for the first Zn(II) binding event of 0.0044 mM, whereas the observed Kd values for the second metal binding event in DapE is 0.0136 mM | Haemophilus influenzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl-LL-2,6-diaminoheptanedioate + H2O | Haemophilus influenzae | - |
succinate + LL-2,6-diaminoheptanedioate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | - |
gene dapE | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl-LL-2,6-diaminoheptanedioate + H2O | - |
Haemophilus influenzae | succinate + LL-2,6-diaminoheptanedioate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DapE | - |
Haemophilus influenzae |
N-succinyl-L,L-diaminopimelic acid desuccinylase | - |
Haemophilus influenzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Haemophilus influenzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Haemophilus influenzae |
General Information | Comment | Organism |
---|---|---|
additional information | DapE residues H355 and H80 are active site ligands, divalent metal binding properties of co-catalytic metallohydrolase active site, overview. Three-dimensional homological structure molecular modeling of N-acetyl-L-ornithine deacetylase, EC 3.5.1.16, from Escherichia coli, using the X-ray crystal structure of the DapE from Haemophilus influenzae, PDB ID 3IC1, as template | Haemophilus influenzae |