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Literature summary for 3.5.1.18 extracted from

  • Reinhard, L.; Mueller-Dieckmann, J.; Weiss, M.S.
    Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of succinyl-diaminopimelate desuccinylase (Rv1202, DapE) from Mycobacterium tuberculosis (2012), Acta Crystallogr. Sect. F, 68, 1089-1093.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene dape, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) chaperone combination 3 (cc3) cells, which co-express the chaperones GroEL and GroES in order to increase the yield of soluble protein expression Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, 0.001-0.002 ml of 3-7 mg/ml protein in 50 mM Bis-Tris, pH 6.0, 200 mM NaCl, 0.5 mM TCEP is mixed with 0.001-0.002 ml reservoir solution, containing 5-10% w/v PEG 4000 or PEG 3350, 35-120 mM ammonium sulfate, 100 mM sodium acetate, pH 4.1-4.6, and equilibrated against 0.9 ml reservoir solution, at room temperature, 1 day, method optimization, X-ray diffraction structure determination and analysis at 2.4-2.58 A resolution, two crystal forms Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-succinyl-LL-2,6-diaminoheptanedioate + H2O Mycobacterium tuberculosis
-
succinate + LL-2,6-diaminoheptanedioate
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WHS9 gene dapE or Rv1202
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-LL-2,6-diaminoheptanedioate + H2O
-
Mycobacterium tuberculosis succinate + LL-2,6-diaminoheptanedioate
-
?

Synonyms

Synonyms Comment Organism
DapE
-
Mycobacterium tuberculosis
S-DAP deacylase
-
Mycobacterium tuberculosis
succinyl-diaminopimelate desuccinylase
-
Mycobacterium tuberculosis

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the seventh reaction step of the succinyl pathway Mycobacterium tuberculosis