Cloned (Comment) | Organism |
---|---|
gene dape, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) chaperone combination 3 (cc3) cells, which co-express the chaperones GroEL and GroES in order to increase the yield of soluble protein expression | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, 0.001-0.002 ml of 3-7 mg/ml protein in 50 mM Bis-Tris, pH 6.0, 200 mM NaCl, 0.5 mM TCEP is mixed with 0.001-0.002 ml reservoir solution, containing 5-10% w/v PEG 4000 or PEG 3350, 35-120 mM ammonium sulfate, 100 mM sodium acetate, pH 4.1-4.6, and equilibrated against 0.9 ml reservoir solution, at room temperature, 1 day, method optimization, X-ray diffraction structure determination and analysis at 2.4-2.58 A resolution, two crystal forms | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl-LL-2,6-diaminoheptanedioate + H2O | Mycobacterium tuberculosis | - |
succinate + LL-2,6-diaminoheptanedioate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WHS9 | gene dapE or Rv1202 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl-LL-2,6-diaminoheptanedioate + H2O | - |
Mycobacterium tuberculosis | succinate + LL-2,6-diaminoheptanedioate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DapE | - |
Mycobacterium tuberculosis |
S-DAP deacylase | - |
Mycobacterium tuberculosis |
succinyl-diaminopimelate desuccinylase | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the seventh reaction step of the succinyl pathway | Mycobacterium tuberculosis |